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Choroidal neovascularization imaging using multiple en face optical coherence tomography angiography image averaging

  • Retinal Disorders
  • Published:
Graefe's Archive for Clinical and Experimental Ophthalmology Aims and scope Submit manuscript

Abstract

Purpose

To determine the effects of averaging five en face optical coherence tomography angiographic (OCTA) images on the quality of the images in eyes with a choroidal neovascularization (CNV).

Methods

Twenty-seven eyes of 25 patients (18 men, 7 women; average age 71.0 years) with a CNV were examined by OCTA (OCT HS-100, Canon. Japan). A 3 × 3-mm image including the CNV was recorded and automatically segmented between the retinal outer layers. Analyses were performed on a single image (S-image) and the average of five single images of the same area (A-images). The region of the CNV was selected by ImageJ, and the peak signal-to-noise ratio (PSNR), the vascular density (VD), fractal dimension (FD), and the noise component using band pass filter (BPF) processing of the S- and A-images of each case were compared.

Results

The average PSNR for the A-images was 14.0 which was significantly higher than the 12.2 for the S-images (P < 0.01). However, the average VD was 33.6% for the S-images and 34.8% for the A-images (P > 0.1). The average FD was 1.67 for the S-images and 1.54 for the A-images (P < 0.01). The mean luminance difference obtained by subtracting the luminance of the A-image from the S-image after BPF processing was 10.41 ± 14.66 db which was positive for all eyes.

Conclusions

The better quality of the A-images of a CNV and absence of a significant difference in the vascular density indicates that the improvement was due to the removal of the same signal levels of the noise component and blood vessels.

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References

  1. Nagiel A, Sadda SR, Sarraf D (2015) A promising future for optical coherence tomography angiography. JAMA Ophthalmol 133:629–630

    Article  PubMed  Google Scholar 

  2. Spaide RF, Klancnik JM Jr, Cooney MJ (2015) Retinal vascular layers imaged by fluorescein angiography and optical coherence tomography angiography. JAMA Ophthalmol 133:45–50

    Article  PubMed  Google Scholar 

  3. Savastano MC, Lumbroso B, Rispoli M (2015) In vivo characterization of retinal vascularization morphology using optical coherence tomography angiography. Retina 35:2196–2203

    Article  PubMed  Google Scholar 

  4. Jia Y, Tan O, Tokayer J et al (2012) Split-spectrum amplitude decorrelation angiography with optical coherence tomography. Opt Express 20:4710–4725

    Article  PubMed  PubMed Central  Google Scholar 

  5. Sakamoto A, Hangai M, Yoshimura N (2008) Spectral-domain optical coherence tomography with mutiple B-scan averaging for enhanced imaging of retinal diseases. Ophthalmology 115:1071–1078

    Article  PubMed  Google Scholar 

  6. Sander B, Larsen M, Thrane L, Hougaard JL, Jørgensen TM (2005) Enhanced optical coherence tomography imaging by multiple scan averaging. Br J Ophthalmol 89:207–212

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Uji A, Balasubramanian S, Lei J, Baghdasaryan E, Al-Sheikh M, Sadda SR (2017) Impact of multiple en face image averaging on quantitative assessment from optical coherence tomography angiography image. Ophthalmology 124:944–952

    Article  PubMed  Google Scholar 

  8. Uji A, Balasubramanian S, Lei J, Baghdasaryan E, Al-Sheikh M, Sadda SR (2017) Choriocapillaris imaging using multiple en face optical coherence tomography angiography image averaging. JAMA Ophthalmol 135:1197–1204

    Article  PubMed  PubMed Central  Google Scholar 

  9. Uji A, Balasubramanian S, Lei J et al (2018) Multiple enface image averaging for enhanced optical coherence tomography angiography imaging. Acta Ophthalmol 96:e820–e827

    Article  PubMed  Google Scholar 

  10. Mo S, Phillips E, Krawitz BD et al (2017) Visualization of radial Peripapillary capillaries using optical coherence tomography angiography: the effect of image averaging. PLoS One 12(1):e0169385

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Spaide RF, Curcio CA (2017) Evaluation of segmentation of the superficial and deep vascular layers of the retina by optical coherence tomography angiography instruments in Normal eyes. JAMA Ophthalmol 135:259–262

    Article  PubMed  Google Scholar 

  12. Rommel F, Siegfried F, Kurz M et al (2018) Impact of correct anatomical slab segmentation on foveal avascular zone measurements by optical coherence tomography angiography in healthy adults. J Curr Ophthalmol 30:156–160

    Article  PubMed  PubMed Central  Google Scholar 

  13. Spaide RF, Fujimoto JG, Waheed NK (2015) Image artifacts in optical coherence tomography angiography. Retina 35:2163–2180

    Article  PubMed  PubMed Central  Google Scholar 

  14. Lauermann JL, Treder M, Heiduschka P, Clemens CR, Eter N, Alten F (2017) Impact of eye-tracking technology on OCT-angiography imaging quality in age-related macular degeneration. Graefes Arch Clin Exp Ophthalmol 255:1535–1542

    Article  CAS  PubMed  Google Scholar 

  15. Lauermann JL, Woetzel AK, Treder M et al (2018) Prevalences of segmentation errors and motion artifacts in OCT-angiography differ among retinal diseases. Graefes Arch Clin Exp Ophthalmol 256:1807–1816

    Article  CAS  PubMed  Google Scholar 

  16. Kundel HL, Polansky M (2003) Measurement of observer agreement. Radiology 228:303–308

    Article  PubMed  Google Scholar 

  17. Kanda Y (2013) Investigation of the freely available easy-to-use software ‘EZR’ for medical statistics. Bone Marrow Transplant 48:452–458

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The authors thank Professor Emeritus Duco Hamasaki of the Bascom Palmer Eye Institute, University of Miami, for his critical discussion and editing of the final manuscript.

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Authors and Affiliations

  1. Department of Ophthalmology, Tokyo Women’s Medical University, 8-1 Kawadacho, Shinjuku-ku, Tokyo, 162-8666, Japan

    Sakura Murakawa, Ichiro Maruko, Taizo Kawano, Taiji Hasegawa & Tomohiro Iida

Authors
  1. Sakura Murakawa
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  2. Ichiro Maruko
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  3. Taizo Kawano
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  4. Taiji Hasegawa
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  5. Tomohiro Iida
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Corresponding author

Correspondence to Ichiro Maruko.

Ethics declarations

The procedures used in this retrospective study conformed to the tenets of the Declaration of Helsinki. The Institutional Review Board of the Tokyo Women’s Medical University approved this study which included OCT and OCTA observations of eyes with macular and retinal disorders including eyes with choroidal neovascularization. All examinations were performed after an informed consent was obtained.

Conflict of interest

Dr. Murakawa has nothing to disclose.

Dr. Maruko reports grants from JSPS KAKENHI (Grant Number JP16K11274), grants and personal fees from Novartis Pharma K.K., personal fees from Bayer Yakuhin, Ltd., personal fees from Santen Pharmaceutical Inc., personal fees from Alcon Japan, Ltd., personal fees from Topcon Co., Ltd., personal fees from Senju Pharmaceutical Co., Ltd., personal fees from NIDEK Co., Ltd., outside the submitted work.

Dr. Kawano has nothing to disclose.

Dr. Hasesgawa has nothing to disclose.

Dr. Iida reports grants and personal fees from Novartis Pharma K.K. (Japan), personal fees from Bayer Yakuhin, Ltd. (Japan), grants and personal fees from Santen Pharmaceutical Co., Ltd. (Japan), grants from Nidek, grants from Kowa, grants and personal fees from Canon, grants and personal fees from Topcon, grants and personal fees from Senju Seiyaku, grants from AMO, grants and personal fees from JFC, outside the submitted work.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the Institutional Review Board of the Tokyo Women’s Medical University and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Murakawa, S., Maruko, I., Kawano, T. et al. Choroidal neovascularization imaging using multiple en face optical coherence tomography angiography image averaging. Graefes Arch Clin Exp Ophthalmol 257, 1119–1125 (2019). https://doi.org/10.1007/s00417-019-04275-5

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  • DOI: https://doi.org/10.1007/s00417-019-04275-5

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