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Plasma metabonomics study of the patients with acute anterior uveitis based on ultra-performance liquid chromatography–mass spectrometry

  • Basic Science
  • Published:
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Abstract

Background

The identification of the biomarkers of patients with acute anterior uveitis (AAU) may allow for a less invasive and more accurate diagnosis, as well as serving as a predictor in AAU progression and treatment response. The aim of this study was to identify the potential biomarkers and the metabolic pathways from plasma in patients with AAU.

Methods

Both plasma metabolic biomarkers and metabolic pathways in the AAU patients versus healthy volunteers were investigated using ultra-performance liquid chromatography–mass spectrometry (UPLC–MS) and a metabonomics approach. The principal component analysis (PCA) was used to separate AAU patients from healthy volunteers as well as to identify the different biomarkers between the two groups. Metabolic compounds were matched to the KEGG, METLIN, and HMDB databases, and metabolic pathways associated with AAU were identified.

Results

The PCA for UPLC–MS data shows that the metabolites in AAU patients were significantly different from those of healthy volunteers. Of the 4,396 total features detected by UPLC–MS, 102 features were significantly different between AAU patients and healthy volunteers according to the variable importance plot (VIP) values (greater than two) of partial least squares discriminate analysis (PLS-DA). Thirty-three metabolic compounds were identified and were considered as potential biomarkers. Meanwhile, ten metabolic pathways were found that were related to the AAU according to the identified biomarkers.

Conclusions

These data suggest that metabolomics study can identify potential metabolites that differ between AAU patients and healthy volunteers. Based on the PCA, PLS-DA, several potential metabolic biomarkers and pathways in AAU patients were found and identified. In addition, the UPLC–MS technique combined with metabonomics could be a suitable systematic biology tool in research in clinical problems in ophthalmology, and can provide further insight into the pathophysiology of AAU.

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Acknowledgments

The present work was supported by the foundation from the National Natural Science Foundation of China (81072961, 81373826), the Development Project of Medicine and Health Science Technology of Shandong Province (2013WS0251), Development Project of Science and Technology of Traditional Chinese Medicine of Shandong Province (2013ZDZK-083). The authors would like to thank the Waters Corporation, especially Yun Wang Ph.D and Lankun Song, for their assistance with UPLC–MS experiments on the research described in this manuscript.

Conflict of interest

The authors declare that they have no conflict or financial interest in the subject matter of this manuscript.

Presentation at conference

The paper abstract was used as written communication on 5th Chinese Congress of Research in Vision and Ophthalmology,CCRVO 2013; Submitted paper abstract to The 18th Congress of Chinese Ophthalmological Society (September 13–17, 2013 Xiamen International Convention and Exhibition Center).

Clinical trial registration

The study was approved by the Eye Institute of Shandong University of TCM, reference number 2012003.

Author information

Authors and Affiliations

  1. Eye Institute of Shandong University of TCM, No. 48#, Ying-Xiong-Shan Road, Jinan, 250002, People’s Republic of China

    Junguo Guo, Hongsheng Bi, Xingrong Wang & Dadong Guo

  2. Affiliated Eye Hospital of Shandong University of TCM, No. 48#, Ying-Xiong-Shan Road, Jinan, 250002, People’s Republic of China

    Tingqin Yan, Hongsheng Bi, Xiaofeng Xie & Xingrong Wang

  3. Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases in Universities of Shandong, Jinan, 250002, People’s Republic of China

    Junguo Guo, Hongsheng Bi & Dadong Guo

  4. Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases of Shandong Province, Jinan, 250002, People’s Republic of China

    Junguo Guo, Hongsheng Bi & Dadong Guo

  5. Experimental Centre of Shandong University of TCM, Jinan, 250355, People’s Republic of China

    Haiqiang Jiang

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  1. Junguo Guo
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  2. Tingqin Yan
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  3. Hongsheng Bi
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  4. Xiaofeng Xie
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Corresponding author

Correspondence to Hongsheng Bi.

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Guo, J., Yan, T., Bi, H. et al. Plasma metabonomics study of the patients with acute anterior uveitis based on ultra-performance liquid chromatography–mass spectrometry. Graefes Arch Clin Exp Ophthalmol 252, 925–934 (2014). https://doi.org/10.1007/s00417-014-2619-1

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  • DOI: https://doi.org/10.1007/s00417-014-2619-1

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