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Anti-Sez6l2 antibody detected in a patient with immune-mediated cerebellar ataxia inhibits complex formation of GluR1 and Sez6l2

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Fig. 1
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References

  1. Hoftberger R, van Sonderen A, Leypoldt F, Houghton D, Geschwind M, Gelfand J, Paredes M, Sabater L, Saiz A, Titulaer MJ, Graus F, Dalmau J (2015) Encephalitis and AMPA receptor antibodies: novel findings in a case series of 22 patients. Neurology 84:2403–2412

    Article  PubMed  PubMed Central  Google Scholar 

  2. Irani SR, Gelfand JM, Al-Diwani A, Vincent A (2014) Cell-surface central nervous system autoantibodies: clinical relevance and emerging paradigms. Ann Neurol 76:168–184

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Ishikawa N, Daigo Y, Takano A, Taniwaki M, Kato T, Tanaka S, Yasui W, Takeshima Y, Inai K, Nishimura H, Tsuchiya E, Kohno N, Nakamura Y (2006) Characterization of SEZ6L2 cell-surface protein as a novel prognostic marker for lung cancer. Cancer Sci 97:737–745

    Article  CAS  PubMed  Google Scholar 

  4. Mitoma H, Adhikari K, Aeschlimann D, Chattopadhyay P, Hadjivassiliou M, Hampe CS, Honnorat J, Joubert B, Kakei S, Lee J, Manto M, Matsunaga A, Mizusawa H, Nanri K, Shanmugarajah P, Yoneda M, Yuki N (2016) Consensus paper: neuroimmune mechanisms of cerebellar ataxias. Cerebellum (London, England) 15:213–232

    Article  CAS  Google Scholar 

  5. Miyazaki T, Hashimoto K, Uda A, Sakagami H, Nakamura Y, Saito SY, Nishi M, Kume H, Tohgo A, Kaneko I, Kondo H, Fukunaga K, Kano M, Watanabe M, Takeshima H (2006) Disturbance of cerebellar synaptic maturation in mutant mice lacking BSRPs, a novel brain-specific receptor-like protein family. FEBS Lett 580:4057–4064

    Article  CAS  PubMed  Google Scholar 

  6. Yaguchi H, Okumura F, Takahashi H, Kano T, Kameda H, Uchigashima M, Tanaka S, Watanabe M, Sasaki H, Hatakeyama S (2012) TRIM67 protein negatively regulates Ras activity through degradation of 80K-H and induces neuritogenesis. J Biol Chem 287:12050–12059

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Yaguchi H, Yabe I, Takahashi H, Okumura F, Takeuchi A, Horiuchi K, Kano T, Kanda A, Saito W, Matsumoto M, Nakayama KI, Hatakeyama S, Sasaki H (2014) Identification of anti-Sez6l2 antibody in a patient with cerebellar ataxia and retinopathy. J Neurol 261:224–226

    Article  PubMed  Google Scholar 

  8. Yaguchi H, Yabe I, Takahashi H, Watanabe M, Nomura T, Kano T, Matsumoto M, Nakayama KI, Watanabe M, Hatakeyama S (2017) Sez6l2 regulates phosphorylation of ADD and neuritogenesis. Biochem Biophys Res Commun 494:234–241

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We are grateful to Prof. Hidenao Sasaki for helpful comments. We thank Shoko Shimizu for preparation of this manuscript. This work was supported by JSPS KAKENHI Grant number 25893006.

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Authors and Affiliations

  1. Department of Neurology, Hokkaido University Graduate School of Medicine, N15 W7, Kita-ku, Sapporo, 060-8638, Japan

    Hiroaki Yaguchi, Ichiro Yabe & Takahiro Kano

  2. Department of Biochemistry, Faculty of Medicine, Graduate School of Medicine, Hokkaido University, Sapporo, Hokkaido, Japan

    Hiroaki Yaguchi, Hidehisa Takahashi, Masashi Watanabe & Shigetsugu Hatakeyama

  3. Department of Neurology, Brain Science Center, Sapporo City General Hospital, Sapporo, Japan

    Hiroaki Yaguchi & Taichi Nomura

  4. Department of Anatomy, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido, Japan

    Masahiko Watanabe

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  1. Hiroaki Yaguchi
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  2. Ichiro Yabe
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Contributions

HY, IY and HT designed and performed experiments, analyzed and interpreted data and drafted the manuscript and figures. MW, TN, TK, MW and SH conceived and designed the study and critically revised the manuscript. All authors have seen and agree with the content of the manuscript. HY and IY contributed equally to this work.

Corresponding author

Correspondence to Hiroaki Yaguchi.

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Conflicts of interest

All authors state that there is no conflict of interest.

Ethical approval

This study was approved by the Institutional Review Board of Hokkaido University Hospital.

Informed consent

Written informed consent was obtained from the patient (Protocol number 012-0167).

Electronic supplementary material

Below is the link to the electronic supplementary material.

415_2018_8785_MOESM1_ESM.eps

Supplementary material 1 (EPS 22285 kb) Supplemental Fig. 1. Immunofluorescence analysis using HeLa cells transfected with the extracellular domain of Sez6l2 (FLAG-Sez6l2(EX)) and the extracellular domain of GluR1 (HA-GluR1(EX)). (A) Immunofluorescence analysis of FLAG-Sez6l2(EX) and HA-GluR1(EX). The cells were fixed and stained with antibodies to FLAG (green), HA (red), and Hoechst33258 (blue).　Immunofluorescence analysis using HeLa cells transfected with the extracellular domain of Sez6l2 (FLAG-Sez6l2(EX)) and the extracellular domain of GluR1 (HA-GluR1(EX)) showed co-localization of Sez6l2(EX) and GluR1(EX). (B) Immunofluorescence analysis of FLAG-Sez6l2(FL) and GluR1(FL). The cells were fixed and stained with antibodies to FLAG (green), GluR1 (red), and Hoechst33258 (blue). Immunofluorescence analysis using HeLa cells transfected with the full length of FLAG-Sez6l2 [FLAG-Sez6l2 (FL)] and the full length of GluR1(GluR1(FL)) showed partial co-localization of Sez6l2(FL) and GluR1(FL)

415_2018_8785_MOESM2_ESM.eps

Supplementary material 2 (EPS 2906 kb) Supplemental Fig. 2. Schema of “inhibition assay of direct binding between Sez6l2 and GluR1 using the patient’s serum or control serum”. Pt, patient; Ct, control; PBS, phosphate buffered saline; IP, immunoprecipitation; IB, immunoblot. Using recombinant proteins of FLAG-Sez6l2(EX) and HA-GluR1(EX) and the patient’s serum and healthy control serum, we tried to check inhibition of direct binding between Sez6l2 and GluR1. Proteins of FLAG-Sez6l2(EX) and HA-GluR1(EX) were used at the same amounts as those shown in Fig. 1. We washed each sample with PBS gently three times in every PBS wash

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Yaguchi, H., Yabe, I., Takahashi, H. et al. Anti-Sez6l2 antibody detected in a patient with immune-mediated cerebellar ataxia inhibits complex formation of GluR1 and Sez6l2. J Neurol 265, 962–965 (2018). https://doi.org/10.1007/s00415-018-8785-z

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  • DOI: https://doi.org/10.1007/s00415-018-8785-z

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