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Validation of the AGCU Expressmarker 16 + 22Y Kit: a new multiplex for forensic application

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Abstract

Genotyping by targeting short tandem repeats (STRs) has been widely used in forensic applications. However, most commercial kits detect autosomal STRs or Y-STRs alone, which waste both time and opportunity. The AGCU Expressmarker 16 + 22Y Kit includes 16 autosomal and 22 Y-chromosomal STR loci and is designed for the forensic science field and obtaining quicker results. Here, we conducted the validation study according to Scientific Working Group on DNA Analysis Methods (SWGDAM) guidelines. Validation of PCR-based studies, species specificity, sensitivity, DNA mixture studies, inhibitors, precision, and sizing accuracy were performed. Furthermore, this system was also tested in 346 random male samples from Han, Hui, Tibetan, and Zhuang populations in China, showing its high power for forensic discrimination in the Chinese population. In addition, this system was able to deal with AMELY deletion cases, which can correctly identify sex in forensic criminal investigations. Our results suggested that the AGCU Expressmarker 16 + 22Y Kit is a useful tool for rapid criminal investigation.

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References

  1. Gettings KB, Kiesler KM, Faith SA, Montano E, Baker CH, Young BA, Guerrieri RA, Vallone PM (2016) Sequence variation of 22 autosomal STR loci detected by next generation sequencing. Forensic Sci Int Genet 21:15–21. https://doi.org/10.1016/j.fsigen.2015.11.005

    Article  CAS  PubMed  Google Scholar 

  2. Rebala K, Wysocka J, Kapinska E, Cybulska L, Mikulich AI, Tsybovsky IS, Szczerkowska Z (2007) Belarusian population genetic database for 15 autosomal STR loci. Forensic Sci Int 173(2-3):235–237. https://doi.org/10.1016/j.forsciint.2007.02.002

    Article  CAS  PubMed  Google Scholar 

  3. Ballantyne KN, Keerl V, Wollstein A, Choi Y, Zuniga SB, Ralf A, Vermeulen M, de Knijff P, Kayser M (2012) A new future of forensic Y-chromosome analysis: rapidly mutating Y-STRs for differentiating male relatives and paternal lineages. Forensic Sci Int Genet 6(2):208–218. https://doi.org/10.1016/j.fsigen.2011.04.017

    Article  CAS  PubMed  Google Scholar 

  4. Pascali VL, Dobosz M, Brinkmann B (1998) Coordinating Y-chromosomal STR research for the Courts. Int J Legal Med 112(1):1

    Article  CAS  Google Scholar 

  5. Kayser M, Sajantila A (2001) Mutations at Y-STR loci: implications for paternity testing and forensic analysis. Forensic Sci Int 118(2-3):116–121

    Article  CAS  Google Scholar 

  6. Li J, Luo H, Song F, Zhang L, Deng C, Yu Z, Gao T, Liao M, Hou Y (2017) Validation of the Microreader 23sp ID system: a new STR 23-plex system for forensic application. Forensic Sci Int Genet 27:67–73. https://doi.org/10.1016/j.fsigen.2016.12.005

    Article  CAS  PubMed  Google Scholar 

  7. McNevin D, Edson J, Robertson J, Austin JJ (2015) Reduced reaction volumes and increased Taq DNA polymerase concentration improve STR profiling outcomes from a real-world low template DNA source: telogen hairs. Forensic Sci Med Pathol 11(3):326–338. https://doi.org/10.1007/s12024-015-9679-3

    Article  CAS  PubMed  Google Scholar 

  8. Gibson-Daw G, Crenshaw K, McCord B (2018) Optimization of ultrahigh-speed multiplex PCR for forensic analysis. Anal Bioanal Chem 410(1):235–245. https://doi.org/10.1007/s00216-017-0715-x

    Article  CAS  PubMed  Google Scholar 

  9. Kraemer M, Prochnow A, Bussmann M, Scherer M, Peist R, Steffen C (2017) Developmental validation of QIAGEN Investigator(R) 24plex QS Kit and Investigator(R) 24plex GO! Kit: two 6-dye multiplex assays for the extended CODIS core loci. Forensic Sci Int Genet 29:9–20. https://doi.org/10.1016/j.fsigen.2017.03.012

    Article  CAS  PubMed  Google Scholar 

  10. Gopinath S, Zhong C, Nguyen V, Ge J, Lagace RE, Short ML, Mulero JJ (2016) Developmental validation of the Yfiler(R) Plus PCR Amplification Kit: an enhanced Y-STR multiplex for casework and database applications. Forensic Sci Int Genet 24:164–175. https://doi.org/10.1016/j.fsigen.2016.07.006

    Article  CAS  PubMed  Google Scholar 

  11. Taylor D, Biedermann A, Samie L, Pun KM, Hicks T, Champod C (2017) Helping to distinguish primary from secondary transfer events for trace DNA. Forensic Sci Int Genet 28:155–177. https://doi.org/10.1016/j.fsigen.2017.02.008

    Article  CAS  PubMed  Google Scholar 

  12. Purps J, Geppert M, Nagy M, Roewer L (2015) Validation of a combined autosomal/Y-chromosomal STR approach for analyzing typical biological stains in sexual-assault cases. Forensic Sci Int Gen 19:238–242. https://doi.org/10.1016/j.fsigen.2015.08.002

    Article  CAS  Google Scholar 

  13. Bender K, Farfan MJ, Schneider PM (2004) Preparation of degraded human DNA under controlled conditions. Forensic Sci Int 139(2-3):135–140. https://doi.org/10.1016/j.forsciint.2003.10.003

    Article  CAS  PubMed  Google Scholar 

  14. Geng T, Mathies RA (2015) Minimizing inhibition of PCR-STR typing using digital agarose droplet microfluidics. Forensic Sci Int Gen 14:203–209. https://doi.org/10.1016/j.fsigen.2014.10.007

    Article  CAS  Google Scholar 

  15. Opel KL, Chung D, McCord BR (2010) A study of PCR inhibition mechanisms using real time PCR. J Forensic Sci 55(1):25–33. https://doi.org/10.1111/j.1556-4029.2009.01245.x

    Article  CAS  PubMed  Google Scholar 

  16. Ye Y, Gao J, Fan G, Liao L, Hou Y (2015) Population genetics for 23 Y-STR loci in Tibetan in China and confirmation of DYS448 null allele. Forensic Sci Int Genet 16:e7–e10. https://doi.org/10.1016/j.fsigen.2014.11.018

    Article  CAS  PubMed  Google Scholar 

  17. Ludeman MJ, Zhong C, Mulero JJ, Lagace RE, Hennessy LK, Short ML, Wang DY (2018) Developmental validation of GlobalFiler PCR amplification kit: a 6-dye multiplex assay designed for amplification of casework samples. Int J Legal Med 132(6):1555–1573. https://doi.org/10.1007/s00414-018-1817-5

    Article  PubMed  PubMed Central  Google Scholar 

  18. Ou X, Chen W, Chen H, Zhao F, Zheng J, Tong D, Chen Y, Chen A, Sun H (2012) Null alleles of the X and Y chromosomal amelogenin gene in a Chinese population. Int J Legal Med 126(4):513–518. https://doi.org/10.1007/s00414-011-0594-1

    Article  PubMed  Google Scholar 

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Funding

This study was supported by the National Natural Science Foundation of China (NSFC, No. 81772030 and 81701866), the Postdoctoral Fund of Sichuan University (No. 2017SCU12049), and the Outstanding Youth Fund of Sichuan University (No. 2016SCU04A07).

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Author notes

  1. Mingkun Xie and Feng Song contributed equally to this work.

Authors and Affiliations

  1. Institute of Forensic Medicine, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, 3-17 Renmin South Road, Chengdu, 610041, Sichuan, China

    Mingkun Xie, Feng Song, Bowen Xie, Shuangshuang Wang & Haibo Luo

  2. Department of Obstetrics, XiangYa Hospital, Central South University, Changsha, China

    Mingkun Xie

  3. Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, Hunan, China

    Jienan Li

  4. Institute of Criminal Investigation, Zhengzhou City Public Security Bureau, Zhengzhou, China

    Weizhi Wang

  5. Institute of Criminal Science and Technology Research, Lanzhou City Public Security Bureau, Lanzhou, China

    Hong Ma

Authors
  1. Mingkun Xie
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  2. Feng Song
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  3. Jienan Li
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  4. Bowen Xie
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  5. Shuangshuang Wang
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  6. Weizhi Wang
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  7. Hong Ma
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  8. Haibo Luo
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Corresponding author

Correspondence to Haibo Luo.

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Informed consent was obtained from all participating donors. This study was approved by the Ethics Committee of Sichuan University.

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The authors declare that they have no conflict of interest.

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Xie, M., Song, F., Li, J. et al. Validation of the AGCU Expressmarker 16 + 22Y Kit: a new multiplex for forensic application. Int J Legal Med 134, 177–183 (2020). https://doi.org/10.1007/s00414-019-02200-3

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  • DOI: https://doi.org/10.1007/s00414-019-02200-3

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