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Evolution and arrangement of the hsp70 gene cluster in two closely related species of the virilis group of Drosophila

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Abstract

To investigate the genetic basis of differing thermotolerance in the closely related species Drosophila virilis and Drosophila lummei, which replace one another along a latitudinal cline, we characterized the hsp70 gene cluster in multiple strains of both species. In both species, all hsp70 copies cluster in a single chromosomal locus, 29C1, and each cluster includes two hsp70 genes arranged as an inverted pair, the ancestral condition. The total number of hsp70 copies is maximally seven in the more thermotolerant D. virilis and five in the less tolerant D. lummei, with some strains of each species exhibiting lower copy numbers. Thus, maximum hsp70 copy number corresponds to hsp70 mRNA and Hsp70 protein levels reported previously and the size of heat-induced puffs at 29C1. The nucleotide sequence and spacing of the hsp70 copies are consistent with tandem duplication of the hsp70 genes in a common ancestor of D. virilis and D. lummei followed by loss of hsp70 genes in D. lummei. These and other data for hsp70 in Drosophila suggest that evolutionary adaptation has repeatedly modified hsp70 copy number by several different genetic mechanisms.

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Acknowledgements

In Russia, research was supported by Russian Grants for Basic Science 0304-48-918 and 0204-49121. In Chicago, research was supported by an International Supplement to NSF grant IBN99-86158 and a Howard Hughes Medical Institute Predoctoral Fellowship. We thank Dr. G.T. Lyozin for technical assistance, Dr. Anneli Hoikkala for providing D. virilis strains, and Dr. B.R. Bettencourt for help in interpreting Drosophila pseudoobscura genomic data and comments on the manuscript.

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Authors and Affiliations

  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, 117984, Moscow, Russia

    Michael B. Evgen’ev, Olga G. Zatsepina, David Garbuz & Elena Zelentsova

  2. Institute of Cell Biophysics, Puschino, Russia

    Michael B. Evgen’ev & Vera Velikodvorskaya

  3. The Committee on Evolutionary Biology, The University of Chicago, 1027 E. 57th Street, Chicago, IL, 60637, USA

    Daniel N. Lerman & Martin E. Feder

  4. Department of Organismal Biology and Anatomy, The Committees on Genetics and on Evolutionary Biology, The University of Chicago, 1027 E. 57th Street, Chicago, IL, 60637, USA

    Martin E. Feder

Authors
  1. Michael B. Evgen’ev
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  2. Olga G. Zatsepina
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  3. David Garbuz
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  4. Daniel N. Lerman
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  5. Vera Velikodvorskaya
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  6. Elena Zelentsova
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  7. Martin E. Feder
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Correspondence to Martin E. Feder.

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Communicated by R. Paro

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Evgen’ev, M.B., Zatsepina, O.G., Garbuz, D. et al. Evolution and arrangement of the hsp70 gene cluster in two closely related species of the virilis group of Drosophila. Chromosoma 113, 223–232 (2004). https://doi.org/10.1007/s00412-004-0312-6

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  • DOI: https://doi.org/10.1007/s00412-004-0312-6

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