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Relative biological effectiveness of 25 and 10 kV X-rays for the induction of chromosomal aberrations in two human mammary epithelial cell lines

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Abstract

Administration of ionizing radiation for diagnostic purposes can be associated with a risk for the induction of tumors. Therefore, particularly with regard to general screening programs, e.g. with mammography, cost-benefit considerations must be discussed including risk estimation depending upon the radiation quality administered. The present study was initiated to investigate the in vitro X-ray energy dependence for the induction of chromosomal aberrations in the two mammary epithelial cell lines, 184A1 and MCF-12A. The induced excess fragments, dicentric chromosomes and centric rings were analyzed and the relative biological effectiveness (RBE) was determined for 10 and 25 kV X-rays relative to 200 kV X-rays. The assumed energy dependence with higher values for 10 kV X-rays was confirmed for the excess fragments, with RBEM values of 1.92 ± 0.26 and 1.40 ± 0.12 for 10 kV X-rays and 1.17 ± 0.12 and 0.97 ± 0.10 for 25 kV photons determined for cell lines 184A1 and MCF-12A, respectively. Meaningful results for the induction of dicentric chromosomes and centric rings were obtained only for higher doses with RBE values of 1.31 ± 0.21 and 1.70 ± 0.29 for 184A1 and 1.08 ± 0.08 and 1.43 ± 0.12 for MCF-12A irradiated with 25 and 10 kV X-rays, respectively.

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Authors and Affiliations

  1. Institute of Radiation Physics, Forschungszentrum Dresden-Rossendorf, P.O. Box 510119, 01314, Dresden, Germany

    Elke Beyreuther, Anna Lehnert, Elisabeth Lessmann & Jörg Pawelke

  2. Department of Radiotherapy and Radiation Oncology, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany

    Wolfgang Dörr

  3. OncoRay, Medical Faculty Carl Gustav Carus, Center for Radiation Research in Oncology, Technische Universität Dresden, Dresden, Germany

    Jörg Pawelke

Authors
  1. Elke Beyreuther
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  2. Wolfgang Dörr
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  3. Anna Lehnert
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  4. Elisabeth Lessmann
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  5. Jörg Pawelke
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Correspondence to Elke Beyreuther.

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Beyreuther, E., Dörr, W., Lehnert, A. et al. Relative biological effectiveness of 25 and 10 kV X-rays for the induction of chromosomal aberrations in two human mammary epithelial cell lines. Radiat Environ Biophys 48, 333–340 (2009). https://doi.org/10.1007/s00411-009-0221-4

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  • DOI: https://doi.org/10.1007/s00411-009-0221-4

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