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A track structure model for simulation of strand breaks in plasmid DNA after heavy ion irradiation

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Abstract

We present a track structure model based on the local dose deposited around heavy ion tracks to explain the cross sections for single-strand and double-strand break induction in plasmid DNA in different aqueous buffers. The model is based only on measurable quantities, namely the effect distribution for inducing strand breaks after x-ray irradiation as a function of dose, and the radial dose distribution of the heavy ion track. The effect of indirect DNA damage mediated by free radicals produced in the water surrounding the DNA is accounted for by allowing the radial dose distribution to be smeared in space by an effective target size corresponding to the squared sum of the geometrical extension of the plasmid molecule and the mean free drift path of the radicals in the buffer solution. Our calculations reproduce well the measured cross sections for single-strand and double-strand break induction in SV40 plasmid DNA in various buffer solutions both as a function of the LET and of the specific energy of the heavy ion.

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Abbreviations

TE buffer :

10−2 mol dm−3 Tris, 10−3 mol dm−3 EDTA, pH 7.9

EO buffer :

5×10−4 mol dm−3 Tris, 10−4 mol dm−3 MgCl2, pH 7.4–7.6.

References

  1. Roots R, Holley W, Chatterjee A, Rachal E, Kraft G (1989) The influence of radiation quality on the formation of DNA breaks. Adv Space Res 9:45–55

    Article  ADS  Google Scholar 

  2. Christensen RC, Tobias CA, Taylor WD (1972) Heavy-ion-induced single- and double-strand breaks in PhiX-174 replicative form DNA. Int J Radiat Biol 22:457–477

    Article  Google Scholar 

  3. Tancher-Scholz G, Kraft G (1999) Influence of radiation quality on the yield of DNA strand breaks in SV40 DNA irradiated in solution. Radiat Res 151:595–604

    Article  Google Scholar 

  4. Katz R, Kobetich EJ (1969) Particle tracks in emulsion. Phys Rev 186:344–351

    Article  ADS  Google Scholar 

  5. Geiß O, Krämer M, Kraft G (1998) Efficiency of thermoluminescent detectors to heavy-charged particles. Nucl Instrum Methods Phys Res B 142:592–598

    Article  Google Scholar 

  6. Spielberger B, Scholz M, Kramer M, Kraft G (2002) Calculation of the x-ray film response to heavy charged particle irradiation. Phys Med Biol 47:4107–4120

    Article  Google Scholar 

  7. Zhang Ch, Dunn DE, Katz R (1985) Radial distribution of dose and cross-sections for the inactivation of dry enzymes and viruses. Radiat Prot Dosim 13:215–218

    Google Scholar 

  8. Scholz M, Kraft G (1996) Track structure and the calculation of biological effects of heavy charged particles. Adv Space Res 18:5–14

    Article  ADS  Google Scholar 

  9. Katz R, Wesely S (1991) Cross sections for single and double strand breaks in SV-40 virus in EO buffer after heavy ion irradiation: experiment and theory. Radiat Environ Biophys 30:81–85

    Article  Google Scholar 

  10. Wingate CL, Baum JW (1976) Measured radial distributions of dose and LET for alpha and proton beams in hydrogen and tissue-equivalent gas. Radiat Res 65:1–19

    Article  Google Scholar 

  11. Varma MN, Baum JW, Kuehner AV (1977) Radial dose, LET, and W for 16O ions in N2 and tissue-equivalent gases. Radiat Res 70:511–518

    Article  Google Scholar 

  12. Varma MN, Baum JW (1980) Energy deposition in nanometer regions by 377 MeV/nucleon20Ne ions. Radiat Res 81:355–363

    Article  Google Scholar 

  13. Varma MN, Baum JW, Kuehner AV (1980) Stopping power and radial dose distribution for 42 MeV bromine ions. Phys Med Biol 25:651–656

    Article  Google Scholar 

  14. Wilson WE, Paretzke HG (1981) Calculation of distributions for energy imparted and ionization by fast protons in nanometer sites. Radiat Res 87:521–537

    Article  Google Scholar 

  15. Hamm RN, Turner JE, Ritchie RH, Wright HA (1985) Calculation of heavy-ion tracks in liquid water. Radiat Res [Suppl 8]:S20-S26

    Article  Google Scholar 

  16. Krämer M, Kraft G (1994) Calculations of heavy-ion track structure. Radiat Environ Biophys 33:91–109

    Article  Google Scholar 

  17. Wilson WE, Nikjoo H (1999) A Monte Carlo code for positive ion track simulation. Radiat Environ Biophys 38:97–104

    Article  Google Scholar 

  18. Kiefer J, Straaten H (1986) A model of ion, track structure based on classical collision dynamics. Phys Med Biol 31:1201–1209

    Article  Google Scholar 

  19. Sonntag C von (1987) The chemical basis of radiation biology. Taylor & Francis, London

    Google Scholar 

  20. Schwarz HA (1969) Applications of the spur diffusion model to the radiation chemistry of aqueous solutions. J Phys Chem 73:1928–1937

    Article  Google Scholar 

  21. Kellerer AM (1977) Microdosimetric concepts relevant to HZE-particles. In: Proceedings of the European Symposium on Life Sciences Research in Space. Cologne Porz

  22. La Verne JA (2000) Track effects of heavy ions in liquid water. Radiat Res 153:487–496

    Article  Google Scholar 

  23. Waligorski MPR, Hamm RN, Katz R (1986) The radial distribution of dose around the path of a heavy ion in liquid water. Nucl Tracks Radiat Meas 11:309–319

    Article  Google Scholar 

  24. Shao C, Saito M, Yu Z (1999) Formation of single- and double-strand breaks of pBR322 plasmid irradiated in the presence of scavengers. Radiat Environ Biophys 38:105–109

    Article  Google Scholar 

  25. Milligan JR, Aguilera JA, Wu CC, Ng JY, Ward JF (1996) The difference that linear energy transfer makes to precursors of DNA strand breaks. Radiat Res 145:442–448

    Article  Google Scholar 

  26. Schmidt JB (1993) Heavy ion induced lesions in DNA: a theoretical model for the initial induction of DNA strand breaks and chromatin breaks. University of California, Berkley

    Google Scholar 

  27. Fulford J, Nikjoo H, Goodhead DT, O’Neill P (2001) Yields of SSB and DSB induced in DNA by Al(K) ultrasoft X-rays and alpha-particles: comparison of experimental and simulated yields. Int J Radiat Biol 77:1053–1066

    Article  Google Scholar 

  28. Kellerer AM, Rossi HH (1978) A generalized formulation of dual radiation action. Radiat Res 75:471–488

    Article  Google Scholar 

  29. Zaider M (1992) The application of the principle of “dual radiation action” in biophysical modelling. In: Chadwick KH, Moschini G, Varma MN. Biophysical modelling of radiation effects. Adam Hilger, Bristol, Philadelphia, New York, pp 37–45

    Google Scholar 

  30. Kampf G (1988) Induction of DNA double-strand breaks by ionizing radiation of different quality and their relevance for cell inactivation. Radiobiol Radiother (Berl) 29:631–658

    Google Scholar 

  31. Heilmann J, Taucher-Scholz G, Kraft G (1995) Induction of DNA double-strand breaks in CHO-K1 cells by carbon ions. Int J Radiat Biol 68:153–162

    Article  Google Scholar 

  32. Brons S, Jakob B, Taucher-Scholz G, Kraft G (2001) Heavy ion production of single- and double-strand breaks in plasmid DNA in aqeous solution. Phys Med 17 [Suppl 1]:217–218

    Google Scholar 

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Authors and Affiliations

  1. Gesellschaft für Schwerionenforschung (GSI), Planckstrasse 1, 64291, Darmstadt, Germany

    S. Brons, G. Taucher-Scholz, M. Scholz & G. Kraft

Authors
  1. S. Brons
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  2. G. Taucher-Scholz
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  3. M. Scholz
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  4. G. Kraft
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Correspondence to S. Brons.

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Published online: 12 March 2003

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Brons, S., Taucher-Scholz, G., Scholz, M. et al. A track structure model for simulation of strand breaks in plasmid DNA after heavy ion irradiation. Radiat Environ Biophys 42, 63–72 (2003). https://doi.org/10.1007/s00411-003-0184-9

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  • DOI: https://doi.org/10.1007/s00411-003-0184-9

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