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Association of immunoexpression of the galectins-3 and -7 with histopathological and clinical parameters in oral squamous cell carcinoma in young patients

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Abstract

An increasing incidence of oral squamous cell carcinoma (OSCC) in individuals younger than 45 years has been observed in recent years. OSCC in younger patients differs in terms of biological behavior and prognosis with the disease being more aggressive than in older patients. The aim of this study was to analyze the immunohistochemical expression of galectins-3 and -7 in 32 cases of OSCC in young patients and to correlate this expression with clinical and morphological parameters. All cases of OSCC of the sample were diagnosed at oncology referral hospitals in Paraíba, Brazil, between 2002 and 2012. Clinical data were obtained from the patient records. Histological malignancy grading systems proposed by Bryne et al. (J Pathol 166:375–381, 1992) and the World Health Organization (In: Pathology and genetics of head and neck tumours: Word Health Organization classification of tumours, 2005) were used for morphological analysis. Immunohistochemistry was performed by the streptavidin–biotin technique using anti-galectin-3 and -7 antibodies. The results were analyzed statistically by the Chi-squared and Fisher exact tests (p < 0.05). Immunoexpression of galectin-3 was observed in 65.6 % of the cases analyzed, but showed no significant association with any of the variables studied (clinical staging; histological malignancy grading systems). Immunoexpression of galectin-7 was observed in 96.9 % of cases and was significantly associated with histological malignancy grading systems (p < 0.05). In conclusion, the results suggest the use of galectin-7 as marker of biological behavior and tumor progression in OSCC in young patients.

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References

  1. Ribeiro ACP, Silva ARS, Simonato LE, Salzedas LMP, Sundefeld MLMM, Soubhia AMP (2009) Clinical and histopathological analysis of oral squamous cell carcinoma in young people: a descriptive study in Brazilians. Br J Oral Maxillofac Surg 47:95–98. doi:10.1016/j.bjoms.2008.05.004

    Article  PubMed  Google Scholar 

  2. Benevenuto TG, Nonaka CFW, Pinto LP, Souza LB (2012) Immunohistochemical comparative analysis of cell proliferation and angiogenic index in squamous cell carcinomas of the tongue between young and older patients. Appl Immunohistochem Mol Morphol 20:291–297. doi:10.1097/PAI.0b013e31823277f6

    Article  PubMed  Google Scholar 

  3. Soudry E, Preis M, Hod R, Hamzany Y, Hadar T, Bahar G, Strenov Y, Shpitzer T (2010) Squamous cell carcinoma of the oral tongue in patients younger than 30 years: clinicopathologic features and outcome. Clin Otolaryngol 35:307–312. doi:10.1111/j.1749-4486.2010.02164.x

    Article  PubMed  CAS  Google Scholar 

  4. Van Monsjou HS, Van Velthuysen MLF, Van Den Brekel MWM, Jordanova ES, Melief CJM, Balm AJM (2012) Human papillomavirus status in young patients with head and neck squamous cell carcinoma. Int J Cancer 130:1806–1812. doi:10.1002/ijc.26195

    Article  PubMed  Google Scholar 

  5. Patel SC, Carpenter WR, Tyree S, Couch ME, Weisller M, Hackman T, Hayes DN, Shores C, Chera BS (2011) Increasing incidence of oral tongue squamous cell carcinoma in young white women, age 18 to 44 years. J Clin Oncol 29:1488–1494. doi:10.1200/jco.2010.31.7883

    Article  PubMed  Google Scholar 

  6. Kaminagakura E, Vartanian GJ, Silva SDS, Santos CR, Kowalski LP (2010) Case-control study on prognostic factors in oral squamous cell carcinoma in young patients. Head Neck 32:1460–1466. doi:10.1002/hed.21618

    Article  PubMed  Google Scholar 

  7. Beena VT, Chauhan I, Heera R, Rajeev R (2011) Oral cancer in young non-habituè females: a report of four cases and review of the literature. Aust Dent J 56:322–327. doi:10.1111/j.1834-7819.2011.01344.x

    Article  PubMed  CAS  Google Scholar 

  8. Falaki F, Dalirsani Z, Pakfetrat A, Falaki A, Saghravanian N, Nosratzehi T, Pazouki M (2011) Clinical and histopathological analysis of oral Squamous cell carcinoma of young patients in Mashhad, Iran: A retrospective study and review of literatures. Med Oral Patol Oral Cir Bucal 16:473–477. doi:10.4317/medoral.16.e473

    Article  Google Scholar 

  9. Alves PM, Godoy GP, Gomes DQ, Medeiros AMC, Souza LB, Silveira EJD, Vasconcelos MG, Queiroz LMG (2011) Significance of galectins-1, -3, -4 and -7 in the progression of squamous cell carcinoma of the tongue. Pathol Res Pract 207:236–240. doi:10.1016/j.prp.2011.02.004

    Article  PubMed  CAS  Google Scholar 

  10. Carvalho MV, Pereira JS, Alves PM, Silveira EJD, Souza LB, Queiroz LMG (2013) Alterations in the immunoexpression of galectins-1, -3 and -7 between different grades of oral epithelial dysplasia. J Oral Pathol Med 42:174–179. doi:10.1111/j.1600-0714.2012.01199.x

    Article  CAS  Google Scholar 

  11. Faria PR, Chammas R, Melo TL, Hsu DK, Liu FT, Nonogaki S, Cardoso SV, Loyola AM (2011) Absence of galectin-3 does not affect the development of experimental tongue carcinomas in mice. Exp Mol Pathol 90:189–193. doi:10.1016/j.yexmp.2010.12.007

    Article  PubMed  Google Scholar 

  12. Kosacka M, Piesiak P, Kowal A, Gołecki M, Jankowska R (2011) Galectin-3 and cyclin D1 expression in non-small cell lung câncer. J Exp Clin Cancer Res 30:2–7. doi:10.1186/1756-9966-30-101

    Article  Google Scholar 

  13. Saussez S, Lorfevre F, Lequeux T, Laurent G, Chantrain G, Vertongen F, Toubeau G, Decastercker C, Kiss R (2008) The determination of the levels of circulating galectin-1 and -3 in HNSCC patients could be used to monitor tumor progression and/or responses to the therapy. Oral Oncol 44:86–93. doi:10.1016/j.oraloncology.2006.12.014

    Article  PubMed  CAS  Google Scholar 

  14. Bryne MS, Koppang HS, Lilleng R, Kjaerheim A (1992) Malignancy grading of the deep invasive margins of oral squamous cell carcinomas has high prognostic value. J Pathol 166:375–381

    Article  PubMed  CAS  Google Scholar 

  15. Barnes L, Eveson JW, Reichart P, Sidransky D (2005) Pathology and genetics of head and neck tumours: Word Health Organization classification of tumours. IARC Press, Lyon (France), pp 122–125

    Google Scholar 

  16. Chiang WF, Liu SY, Fang LY, Lin CN, Wu MH, Chen YC, Chen YL, Jin YT (2008) Overexpression of galectin-1 at the tumor invasion front is associated with poor prognosis in early stage oral squamous cell carcinoma. Oral Oncol 44:325–334. doi:10.1016/j.oraloncology.2007.03.004

    Article  PubMed  CAS  Google Scholar 

  17. Honjo Y, Inohara H, Akahani S, Yoshii T, Takenaka Y, Yoshida J, Hattori K, Tomiyama Y, Raz A, Kubo T (2000) Expression of cytoplasmic galectin-3 as a prognostic marker in tongue carcinoma. Clin Cancer Res 6:4635–4640

    PubMed  CAS  Google Scholar 

  18. Than TH, Swethadri GK, Wong J, Ahmad T, Jamil D, Maganlal RK (2008) Expression of galectin-3 and galectin-7 in thyroid malignancy as potential diagnostic indicators. Singapore Med J 49:333–338

    PubMed  CAS  Google Scholar 

  19. Mallet Y, Avalos N, Ridant AL, Gangloff P, Moriniere S, Rame JP, Poissonnet G, Makeieff M, Cosmidis A, Babin E, Barry B, Fournier C (2009) Head and neck cancer in young people: a series of 52 SCCs of the oral tongue in patients aged 35 years or less. Acta Otolaryngol 129:1503–1508. doi:10.3109/00016480902798343

    Article  PubMed  Google Scholar 

  20. Alsharif MJ, Jiang WA, He S, Zhao Y, Shan Z, Chen X (2009) Gingival squamous cell carcinoma in young patients: report of a case and review of the literature. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 107:696–700. doi:10.1016/j.tripleo.2008.12.048

    Article  PubMed  Google Scholar 

  21. Santos-Silva AR, Ribeiro ACP, Soubhia AMP, Miyahara GI, Carlos R, Speight PM, Hunter KD, Torres-Rendon A, Vargas PA, Lopes MA (2011) High incidences of DNA ploidy abnormalities in tongue squamous cell carcinoma of young patients: an international collaborative study. Histopathology 58:1127–1135. doi:10.1111/j.1365-2559.2011.03863.x

    Article  PubMed  Google Scholar 

  22. O’regan EM, Toner ME, Finn SP, Fan CY, Ring M, Hagmar B, Timon C, Smyth P, Cahill S, Flavin R, Sheils OM, O’Leary JJ (2008) p16INK4A genetic and epigenetic profiles differ in relation to age and site in head and neck squamous cell carcinomas. Hum Pathol 39:452–458. doi:10.1016/j.humpath.2007.08.004

    Article  PubMed  Google Scholar 

  23. Vered M, Dayan D, Dobriyan A, Yahalom R, Shalmon B, Barshack I, Bedrin L, Talmi YP, Taicher S (2010) Oral tongue squamous cell carcinoma: recurrent disease is associated with histopathologic risk score and young age. J Cancer Res Clin Oncol 136:1039–1048. doi:10.1007/s00432-009-0749-3

    Article  PubMed  Google Scholar 

  24. Dumic J, Dabelic S, Flögel M (2006) Galectin-3: an open-ended story. Biochim Biophys Acta 1760:616–635. doi:10.1016/j.bbagen.2005.12.020

    Article  PubMed  CAS  Google Scholar 

  25. Kapucuoglu N, Basakb PY, Bircana S, Serta S, Akkayab VB (2009) Immunohistochemical galectin-3 expression in non-melanoma skin cancers. Pathol Res Pract 205:97–103. doi:10.1016/j.prp.2008.09.001

    Article  PubMed  Google Scholar 

  26. Delorge S, Saussez S, Pelc P, Devroede B, Marchant H, Burchert M, Danguy A, Salmon I, Gabius HJ, Kiss R, Hassid S (2000) Correlation of galectin-3/galectin-3-binding sites with low differentiation status in head and neck squamous cell carcinomas. Otolaryngol Head Neck Surg 122:834–841. doi:10.1016/S0194-5998(00)70010-6

    Article  PubMed  CAS  Google Scholar 

  27. Wang LP, Chen SW, Zhuang SM, Li H, Song M (2013) Galectin-3 accelerates the progression of oral tongue squamous cell carcinoma via a Wnt/β-catenin-dependent pathway. Pathol Oncol Res 19:461–474. doi:10.1007/s12253-013-9603-7

    Article  PubMed  Google Scholar 

  28. Zhu XI, Ding M, Yu M, Feng M, Tan L, Zhao F (2010) Identification of galectin-7 as a potential biomarker for esophageal squamous cell carcinoma by proteomic analysis. BMC Cancer 10:2–12. doi:10.1186/1471-2407-10-290

    Article  Google Scholar 

  29. Demers M, Magnaldo T, St-Pierre Y (2005) Novel function for galectin-7: promoting tumorigenesis by up regulating MMP-9 gene expression. Cancer Res 65:5205–5210. doi:10.1158/0008-5472.CAN-05-0134

    Article  PubMed  CAS  Google Scholar 

  30. Matsukawa S, Morita KI, Negishi A, Harada H, Nakajima Y, Shimamoto H, Hirofumi T, Tanaka K, Oho M, Yamada T, Omura K (2014) Galectin-7 as a potential predictive marker of radio-therapy resistance in oral squamous cell carcinoma. Cancer Med 3:349–361. doi:10.1002/cam4.195

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  31. Kuwabara I, Kuwabara Y, Yang RY, Schuler M, Green DR, Zuraw BL, hsu DK, Liu FT (2002) Galectin-7 (PIG1) exhibits pro-apoptotic function through JNK activation and mitochondrial cytochrome C release. JBC 277:3487–3497. doi:10.1074/jbc.M109360200

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank the National Council for Scientific and Technological Development-CNPq for financial support (n.485788/2013-6).

Conflict of interest

The authors declare that they have no conflict of interests.

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Authors and Affiliations

  1. Department of Dentistry, Oral Pathology, State University of Paraiba, Juvêncio Arruda, s/n- Bodocongó, Campina Grande, PB, CEP: 58429-600, Brazil

    Janaina Almeida Mesquita, Manuel Antônio Gordon-Nunez, Gustavo Pina Godoy, Cassiano Franscisco Weege Nonaka & Pollianna Muniz Alves

  2. Department of Dentistry, Oral Pathology, Federal University of Rio Grande do Norte, Natal, RN, Brazil

    Lélia Maria Guedes Queiroz & Éricka Janine Dantas Silveira

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  1. Janaina Almeida Mesquita
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  2. Lélia Maria Guedes Queiroz
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Correspondence to Pollianna Muniz Alves.

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Mesquita, J.A., Queiroz, L.M.G., Silveira, É.J.D. et al. Association of immunoexpression of the galectins-3 and -7 with histopathological and clinical parameters in oral squamous cell carcinoma in young patients. Eur Arch Otorhinolaryngol 273, 237–243 (2016). https://doi.org/10.1007/s00405-014-3439-y

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  • DOI: https://doi.org/10.1007/s00405-014-3439-y

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