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Theophylline induces changes in the electro-olfactogram of the mouse

  • Rhinology
  • Published:
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Abstract

Options for the treatment of hyposmia are limited; available therapies do not provide a long-lasting effect. A recent study suggests that an unspecific phosphodiesterase inhibitor (PDE-I) increases olfactory sensitivity due to interaction with the signal transduction in the olfactory epithelium. The aim of the present study was to investigate whether theophylline, an unspecific PDE-I, evokes changes in the electro-olfactogram (EOG) which would support the hypothesis of a drug-related impact on signal transduction. In addition, the uptake of topically administered theophylline in the olfactory epithelium should be investigated. EOG was obtained in 29 samples of supravital mouse olfactory epithelia. Olfactory stimulation (phenylethyl alcohol, PEA and hydrogen sulfide, H2S) was performed using an air-dilution olfactometer. Theophylline concentration in the olfactory epithelium of five samples was measured by means of high pressure liquid chromatography. Administration of theophylline resulted in a tendency towards smaller EOG amplitudes (p = 0.055), being reduced by 13 and 25% in response to stimulation with PEA or H2S, respectively. In comparison to the application of Ringer’s solution, theophylline resulted in a significant (p = 0.031) decrease of the EOG amplitude. Latency was not significantly (p = 0.10) influenced by drug administration. The theophylline concentration in the olfactory epithelium ranged from 0.21 to 1.53 μg/mg. Theophylline seems to be taken up into the olfactory epithelium of supravital mice and to interact with the olfactory signal transduction.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Smell and Taste Clinic, Department of Otorhinolaryngology, University of Dresden Medical School, Fetscherstr. 74, 01307, Dresden, Germany

    Volker Gudziol, Martin Witt & Thomas Hummel

  2. Institute of Legal Medicine, University of Dresden Medical School, Dresden, Germany

    Jörg Pietsch

  3. Department of Anatomy, University of Rostock Medical School, Rostock, Germany

    Martin Witt

Authors
  1. Volker Gudziol
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  2. Jörg Pietsch
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  3. Martin Witt
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  4. Thomas Hummel
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Correspondence to Volker Gudziol.

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Gudziol, V., Pietsch, J., Witt, M. et al. Theophylline induces changes in the electro-olfactogram of the mouse. Eur Arch Otorhinolaryngol 267, 239–243 (2010). https://doi.org/10.1007/s00405-009-1076-7

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  • DOI: https://doi.org/10.1007/s00405-009-1076-7

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