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Ani s 1 and Ani s 7 recombinant allergens are able to differentiate distinct Anisakis simplex-associated allergic clinical disorders

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Abstract

Diagnosis in gastro-allergic anisakiasis (GAA) is straightforward, when clinical history is combined with further allergological evaluation of specific IgE by means of skin prick test and serum specific IgE. In Anisakis simplex sensitisation associated chronic urticaria (CU+), clinical evaluation of possible previous parasitism is difficult, and positive serum specific IgE could be due to cross-reactivity or other unknown factors. In this study, we evaluated the association between IgE seropositivity to the recombinant allergens Ani s 1 and Ani s 7 and several A. simplex-associated allergic disorders. Twenty-eight patients with GAA and 40 patients with CU+ were studied and their IgE responses were compared with a control group composed of patients with chronic urticaria not sensitized to A. simplex (CU−) according to the skin prick test, as well as a group of 15 healthy subjects not referring urticaria or currently A. simplex associated symptoms. 82.1% of GAA patients and 42.5% of CU+ patients were positive for Ani s 1 (P < 0.001), while the Ani s 7 allergen was recognized by 92.9 and 92.5% of sera from patients with GAA and CU+, respectively. The combined positivity obtained for both allergens reached 100% in GAA, and 95% in CU+. IgE determinations to Ani s 1 and Ani s 7 allergens are useful to diagnose the Anisakis infections and to differentiate among several A. simplex-associated allergic disorders. The IgE responses to Ani s 1 are mainly associated with GAA, while this molecule cannot be considered a major allergen in CU+ patients.

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Abbreviations

GAA:

Gastro-allergic anisakiasis

CU+:

Anisakis simplex sensitisation associated chronic urticaria

CU−:

Chronic urticaria without associated sensitisation against Anisakis simplex

SPT:

Skin prick test

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Acknowledgments

The study was funded by grants from Fundación Sociedad Española de Alergología e Inmunología Clínica (SEAIC) 2009 and Fundación Mutua Madrileña 2009. We thank Laura Fernández Gámez for technical support.

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Authors and Affiliations

  1. Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense, Madrid, Spain

    C. Cuéllar, V. Fernández-Fígares & M. Rodero

  2. Servicio de Alergia, Instituto de Investigación Sanitaria, Hospital Universitario de La Princesa, C/Diego de León, 62, 28006, Madrid, Spain

    A. Daschner, A. Valls & C. De Frutos

  3. Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, Santiago de Compostela, Spain

    A. M. Anadón & F. M. Ubeira

  4. Servicio de Parasitología, Centro Nacional de Microbiología, Majadahonda, Spain

    E. Rodríguez & T. Gárate

Authors
  1. C. Cuéllar
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  2. A. Daschner
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  3. A. Valls
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  4. C. De Frutos
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  5. V. Fernández-Fígares
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  6. A. M. Anadón
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  7. E. Rodríguez
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  8. T. Gárate
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  9. M. Rodero
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  10. F. M. Ubeira
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Corresponding author

Correspondence to A. Daschner.

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Cuéllar, C., Daschner, A., Valls, A. et al. Ani s 1 and Ani s 7 recombinant allergens are able to differentiate distinct Anisakis simplex-associated allergic clinical disorders. Arch Dermatol Res 304, 283–288 (2012). https://doi.org/10.1007/s00403-012-1206-8

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  • DOI: https://doi.org/10.1007/s00403-012-1206-8

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