Abstract
Examining the patients with a new variant of endemic pemphigus foliaceus (EPF) in El Bagre, Colombia, (El Bagre-EPF), we noted several polymorphic clinical lesions around their axillary areas. Based on our clinical findings and on previous histopathological studies on the skin of these patients that showed abnormalities in their sweat glands, and the presence of mercuric selenides and iodines by autometallography assay, we decided to investigate immunoreactivity to the sweat glands in these patients. We tested for autoreactivity utilizing direct and indirect immunofluorescence (DIF, IIF). To be able to distinguish between non-specific immune deposits and real autoimmune response, and knowing that sweat glands have some intrinsic autofluorescence for the presence of lipofuscin granules (that naturally fluoresce under the UV light microscope), as well as by the presence of secretory IgA, we used simultaneously immunohistochemistry (IHC). We tested ten El Bagre-EPF patients, ten healthy controls from the endemic area and ten healthy controls from the United States. We were able to visualize a specific autoreactivity to sweat glands in 8/10 cases of El Bagre-EPF by DIF, IIF and by IHC. In addition when using anti-human monoclonal antibodies to CD3, CD68, and CD20, we confirmed the presence of several specific immune responses in situ, an around the sweat glands. No healthy control cases yielded positive findings. In some chronic cases, decrease and sometimes a complete absence of sweat glands and other skin appendices was found. In addition to this, sclerodermoid changes or early sclerodermatous changes sometimes extending into the adipose tissue as a membranous lipodystrophy were observed. Autoreactivity to the neurovascular components around the sweat glands were also observed. Our data demonstrate for the first time that there is immunoreactivity toward sweat glands in El Bagre-EPF patients that seems to destroy some of these structures.
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Abréu-Vélez AM, Abréu-Vélez CE, Patiño PJ et al (2003) The tryptic cleavage product of the mature form of the bovine desmoglein 1 ectodomain is one of the antigen moieties immunoprecipitated by all sera from symptomatic patients affected by a new variant of endemic pemphigus. Eur J Dermatol 13:359–366
Abréu-Vélez AM, Beutner E, Montoya F et al (2003) Analyses of autoantigens in a new form of endemic pemphigus foliaceus in Colombia. J Am Acad Dermatol 49:609–614
Abréu-Vélez AM, Hashimoto T, Tobón S et al (2003) A unique form of endemic pemphigus in Northern Colombia. J Am Acad Dermatol 4:580–599
Abréu-Vélez AM, Warfvinge G, Leon-Herrera W et al (2003) Detection of mercury and other undetermined materials in skin biopsies of endemic pemphigus foliaceus. Am J Dermatopathol 25:384–391
Abréu-Vélez AM, Yepes MM, Patiño PJ et al (2004) A cost-effective, sensitive and specific enzyme-linked immunosorbent assay useful for detecting a heterogeneous antibody population in sera from people suffering a new variant of endemic pemphigus. Arch Dermatol Res 295:434–441
Barnadas MA, CurelL R, Alomar A et al (2009) Paraneoplastic pemphigus with negative direct immunofluorescence in epidermis or mucosa but positive findings in adnexal structures. J Cutan Pathol 36:34–38
Beer GM, Baumüller S, Zech N et al (2007) Immunohistochemical differentiation and localization analysis of sweat glands in the adult human axilla. Plast Reconstr Surg 119:1124–1125
Beutner EH, Prigenzi LS, Hale W et al (1968) Immunofluorescence studies of autoantibodies to intercellular areas of epithelia in Brazilian pemphigus foliaceus. Proc Soc Exp Biol Med 127:81–86
Dantzig P (1973) Circulating antibodies in cicatricial pemphigoid. Arch Dermatol 108:264–266
Hans-Filho G, Dos Santos V, Katayama JH et al (1996) An active focus of high prevalence of fogo selvagem on an Amerindian reservation in Brazil. J Invest Dermatol 107:68–75
Hashimoto K, Moiin A, Chang MW et al (1996) Sudoriferous acrosyringeal acantholytic disease. A subset of Grover’s disease. J Cutan Pathol 23:151–164
Hashimoto K (1978) Ultrastructure of human apocrine glands In: Jarrett A (ed) Pathology and pathophysiology of the skin, vol 5, Academic Press, London, pp 1575–1965
Hudson PM, Black MM (1975) Cicatricial pemphigoid: immunofluorescent findings. Proc R Soc Med 68:757–758
Johansson EA, Kanerva L, Niemi K et al (1982) Generalized argyria with low ceruloplasmin and copper levels in the serum. A case report with clinical and microscopical findings and a trial of penicillamine treatment. Clin Exp Dermatol 7:169–176
Jones CJ, Quinton PM (1989) Dye-coupling compartments in the human eccrine sweat gland. Am J Physiol 256(3 Pt 1):C478–C485
Levy DM, Rowley DA, Abraham RR (1991) Changes in cholinergic sweat gland activation in diabetic neuropathy identified by computerized sweat spot analysis. Diabetologia 34:807–812
Lienesch DW, Mutasim DF, Singh RR (2003) Neutrophilic eccrine hidradenitis mimicking cutaneous vasculitis in a lupus patient: a complication of cyclophosphamide. Lupus 12:707–709
Montagna W, Parakkal PF (1974) In: The structure and function of skin, 3rd edn. Academic Press, London
Nomura K, Hashimoto I (1997) Eccrine syringofibroadenomatosis in two patients with bullous pemphigoid. Dermatology 195:309–310
Pickles RJ, Brayden DJ, Cuthbert AW (1991) Synchronous transporting activity in epithelial cells in relation to intracellular calcium concentration. Proc Biol Sci 245:53–58
Shu S, Beutner EH (1973) Isolation and characterization of antigens reactive with pemphigus antibodies. J Invest Dermatol 61:270–276
Valenzuela R, Bergfeld WF, Deodhar SD (1946) In: Interpretation of immunofluorescent patterns in skin diseases. American Society of Clinical Pathologist Press, Chicago, pp 43–45
Ylva Y, Grams YY, Whitehead L et al (2005) On-line diffusion profile of a lipophilic model dye in different depths of a hair follicle in human scalp skin. J Invest Dermatol 125:775–782
Acknowledgments
Georgia Dermatopathology Associates (MSH), Atlanta, GA, USA. The El Bagre-EPF samples were collected from previous grants from the Embassy of Japan in Colombia, DSSA, U. de A. and Mineros de Antioquia SA, (AMAV), Medellin, Colombia, South America.
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Abreu-Velez, A.M., Howard, M.S., Hashimoto, K. et al. Autoantibodies to sweat glands detected by different methods in serum and in tissue from patients affected by a new variant of endemic pemphigus foliaceus. Arch Dermatol Res 301, 711–718 (2009). https://doi.org/10.1007/s00403-009-0972-4
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DOI: https://doi.org/10.1007/s00403-009-0972-4