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Differentiation-regulated expression of Toll-like receptors 2 and 4 in HaCaT keratinocytes

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Abstract

Toll-like receptors (TLRs) play an important role in the recognition of pathogens in keratinocytes. In this study, we investigated whether the differentiation state of HaCaT keratinocytes correlates with the expression of TLR2 and TLR4 genes. The expression levels of TLR2 and TLR4 in a HaCaT differentiation model system were determined using quantitative real-time RT-PCR (Q-RT-PCR) and flow cytometry. The progression of keratinocyte differentiation was monitored by determining the level of involucrin gene expression using Q-RT-PCR. The expression levels of TLR2 and TLR4 increased with the stage of differentiation and there were strong correlations between the expression level of the involucrin gene and those of the TLR2 gene (r=0.809, P<0.0001) and the TLR4 gene (r=0.568, P<0.02). Increased cell surface expression of TLR2 and TLR4 was also found in differentiated HaCaT keratinocytes by flow cytometric analysis. Our findings suggest that upregulation of TLR expression during differentiation in keratinocytes could be a part of the differentiation process of keratinocytes and could have biological significance in protecting skin against microbes.

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Fig. 1A–C
Fig. 2A, B

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Acknowledgements

This work was supported by the OTKA grants T042738, T032498, EU5 QLK4-CT-2001-00366 and FKFP 0222/2000. Márta Széll was supported by the Bolyai Foundation of the Hungarian Academy of Sciences.

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Authors and Affiliations

  1. Department of Dermatology and Allergology, University of Szeged, Korányi fasor 6., H-6701, Szeged, Hungary

    Andor Pivarcsi, Andrea Koreck, Laszlo Bodai, Csilla Szeg, Nóra Belső, Anna Kenderessy-Szabó, Zsuzsanna Bata-Csörgő, Attila Dobozy & Lajos Kemény

  2. Dermatological Research Group of the Hungarian Academy of Sciences, Szeged, Hungary

    Andor Pivarcsi, Márta Széll, Zsuzsanna Bata-Csörgő, Attila Dobozy & Lajos Kemény

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  1. Andor Pivarcsi
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  2. Andrea Koreck
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  3. Laszlo Bodai
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  4. Márta Széll
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  5. Csilla Szeg
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  6. Nóra Belső
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  7. Anna Kenderessy-Szabó
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  8. Zsuzsanna Bata-Csörgő
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  9. Attila Dobozy
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  10. Lajos Kemény
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Correspondence to Andor Pivarcsi.

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Pivarcsi, A., Koreck, A., Bodai, L. et al. Differentiation-regulated expression of Toll-like receptors 2 and 4 in HaCaT keratinocytes. Arch Dermatol Res 296, 120–124 (2004). https://doi.org/10.1007/s00403-004-0475-2

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  • DOI: https://doi.org/10.1007/s00403-004-0475-2

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