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Human parathyroid hormone-related protein and human parathyroid hormone receptor type 1 are expressed in human medulloblastomas and regulate cell proliferation and apoptosis in medulloblastoma-derived cell lines

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Abstract

Human parathyroid hormone-related protein (hPTHrP), identified in patients with paraneoplastic hypercalcemia and expressed by different cell types during development and adult life, plays important roles in many human neoplasms. Immunohistochemical and RT-PCR analyses of hPTHrP and human parathyroid hormone receptor type 1 (PTHR-1) in primary medulloblastoma confirmed their expression in both classic and desmoplastic variants at RNA and protein levels. To evaluate the functional role of hPTHrP, DAOY and D283 medulloblastoma and U87MG glioma cells, expressing high levels of hPTHrP and PTHR-1, were treated with anti-sense oligonucleotides for hPTHrP. Anti-sense treatment produced in all cell lines a decrease of cell proliferation and clonogenic activity and an increase of apoptosis, while addition of exogenous hPTHrP (1–37) prevented these effects. Anti-sense induced the increase of Caspase-3, Fas (CD95) mRNAs and Bax/Bcl-2 mRNA ratio after 12 h of cell treatment. Exogenous hPTHrP (1–37) increased intracellular Ca2+ concentration in DAOY cells as revealed by FURA. Anti-sense treated cells showed a significant decrease of steady-state levels of intracellular Ca2+, which was reverted by addition of exogenous hPTHrP (1–37). This study indicates that hPTHrP and PTHR-1 are expressed in medulloblastoma and could promote tumor growth, protecting cells from apoptosis.

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Acknowledgments

This work was supported in part by grant D.1. 2004 to F.O.R. from the Università Cattolica del S. Cuore within its program of promotion and diffusion of scientific research.

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Authors and Affiliations

  1. Department of Pathology, Catholic University, Rome, Italy

    Marco Gessi, Paola Lanza & Libero Lauriola

  2. Division of Neuropathology, National Neurological Institute “C.Besta”, Milan, Italy

    Marco Gessi

  3. Institute of Human Anatomy and Cellular Biology, Catholic University, Rome, Italy

    Giovanni Monego

  4. Institute of General Pathology, Catholic University, Rome, Italy

    Gabriella Calviello

  5. Department of Experimental Medicine and Pathology, University of Rome “La Sapienza”, Rome, Italy

    Felice Giangaspero

  6. Institute of Biochemistry, Catholic University, Rome, Italy

    Andrea Silvestrini

  7. Institute of Histology, Catholic University, Largo F.Vito 1, 00168, Rome, Italy

    Franco O. Ranelletti

  8. IRCCS Pozzilli, (IS), Italy

    Felice Giangaspero

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  1. Marco Gessi
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Correspondence to Franco O. Ranelletti.

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M. Gessi and G. Monego contributed equally to this work.

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Gessi, M., Monego, G., Calviello, G. et al. Human parathyroid hormone-related protein and human parathyroid hormone receptor type 1 are expressed in human medulloblastomas and regulate cell proliferation and apoptosis in medulloblastoma-derived cell lines. Acta Neuropathol 114, 135–145 (2007). https://doi.org/10.1007/s00401-007-0212-y

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  • DOI: https://doi.org/10.1007/s00401-007-0212-y

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