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Bifidobacterium pseudocatenulatum CECT7765 promotes a TLR2-dependent anti-inflammatory response in intestinal lymphocytes from mice with cirrhosis

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Abstract

Background

Intestinal homeostasis plays an important role in bacteria-derived complications in cirrhosis. Intestinal lymphocytes are responsible for immune effector functions and can be modulated by certain probiotics. We evaluate the interaction between Bifidobacterium pseudocatenulatum CECT7765 and intestinal lymphocytes in mice with cirrhosis.

Animals and methods

Cirrhosis was induced by intragastrical administration of carbon tetrachloride in Balb/C mice. One week prior to laparotomy, animals received B. pseudocatenulatum CECT7765 (107, 109 or 1010 cfu/daily) or placebo. Chemokine receptor and cytokine expression were evaluated in intestinal lymphocytes. Gut permeability was studied by FITC-LPS recovery in vivo. Luminal antigens, inflammation and functional markers were evaluated in liver samples.

Results

Bifidobacterium pseudocatenulatum CECT7765 decreased the expression of pro-inflammatory chemokine receptors CCR6, CCR9, CXCR3 and CXCR6 in intestinal lymphocytes from cirrhotic mice in a concentration-dependent manner. The bifidobacterial strain induced a shift towards an anti-inflammatory cytokine profile in this cell subset. B. pseudocatenulatum CECT7765-induced inflammatory modulation was TLR2-mediated, as in vitro TLR2 blockade inhibited the reduction of TNF-alpha and its receptors and the increase of IL-10 and IL-10 receptor secretion. The recovery rate of administered fluorescence-labelled endotoxin was significantly and dose-dependently lowered with the bifidobacterial strain. The reduced intestinal permeability was associated with a decreased burden of bacterial antigens in the liver of mice treated with B. pseudocatenulatum CECT7765. Liver function and inflammation were improved with the use of the bifidobacterial strain at the highest dose tested (1010 cfu).

Conclusion

Bifidobacterium pseudocatenulatum CECT7765 improves gut homeostasis and prevents gut-derived complications in experimental chronic liver disease.

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Acknowledegments

This work has been supported by grants PI13/1443 from the Instituto de Salud Carlos III, Madrid, Spain, and AGL2011-25169 from MINECO, Spain; Moratalla A. is recipient of a grant ACIF-2012/015, Consellería d’Educació, Generalitat Valenciana, Spain. A. Moya is recipient of a FPU scholarship from MINECO, Spain.

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

Author information

Authors and Affiliations

  1. CIBERehd, Instituto de Salud Carlos III, Madrid, Spain

    Alba Moratalla, Isabel Gómez-Hurtado, Pedro Zapater, José M. González-Navajas, José Such & Rubén Francés

  2. FISABIO, Hospital General Universitario de Alicante, Alicante, Spain

    Alba Moratalla, José M. González-Navajas & Rubén Francés

  3. Grupo Ecología Microbiana, Nutrición y Salud, IATA-CSIC, Valencia, Spain

    Ángela Moya-Pérez, Eva Maria Gómez Del Pulgar & Yolanda Sanz

  4. Servicio de Farmacología Clínica, Hospital General Universitario de Alicante, Alicante, Spain

    Pedro Zapater

  5. Servicio de Anatomía Patológica, Hospital General Universitario de Alicante, Alicante, Spain

    Gloria Peiró

  6. Departamento de Medicina Clínica, Universidad Miguel Hernández, San Juan, Spain

    José Such & Rubén Francés

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  1. Alba Moratalla
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  2. Isabel Gómez-Hurtado
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Correspondence to Rubén Francés.

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Moratalla, A., Gómez-Hurtado, I., Moya-Pérez, Á. et al. Bifidobacterium pseudocatenulatum CECT7765 promotes a TLR2-dependent anti-inflammatory response in intestinal lymphocytes from mice with cirrhosis. Eur J Nutr 55, 197–206 (2016). https://doi.org/10.1007/s00394-015-0837-x

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  • DOI: https://doi.org/10.1007/s00394-015-0837-x

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