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Probiotic Lactobacillus casei Zhang ameliorates high-fructose-induced impaired glucose tolerance in hyperinsulinemia rats

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European Journal of Nutrition Aims and scope Submit manuscript

Abstract

Aim

To evaluate the preventive and therapeutic effects of Lactobacillus casei Zhang on impaired glucose tolerance (IGT) by using fructose-induced hyperinsulinemia rats.

Methods

Rats were fed 25 % fructose solution for hyperinsulinemia with L. casei Zhang for prevention or therapy. Serum levels of insulin, glucagon-like peptide-2 (GLP-2), osteocalcin, malondialdehyde (MDA), total intestinal bile acids and hepatic glycogen contents were determined by assay kits. The major bacteria from feces and liver expression of adiponectin receptor 2 (AdipoR2), liver X receptor-α (LXR-α), peroxisome proliferator-activated receptor gamma (PPAR-γ) and vitamin K epoxide reductase complex subunit 1 mRNA were assessed by RT-PCR. Pancreas injury was evaluated by histological analysis.

Results

Lactobacillus casei Zhang significantly increased numbers of Lactobacillus and Bifidobacterium and decreased Clostridium in the intestine (p < 0.01). Meanwhile, liver glycogen contents were significantly decreased (p < 0.05). In preventive group, accompanied by significantly lower insulin and GLP-2 levels (p < 0.05), L. casei Zhang prevented rats from an increase in oral glucose tolerance area under curve (AUC) which was significant in hyperinsulinemia group (p < 0.05). In therapeutic group, L. casei Zhang administration possessed improved glucose tolerance (p < 0.05), which were associated with increased osteocalcin level (p < 0.01), improved intestinal bile acids secretion (p = 0.060), decreased serum MDA levels (p < 0.05) and upregulation of LXR-α, PPAR-γ and AdipoR2 gene expression, as well as an increase in Bacteroides fragilis (p < 0.05).

Conclusions

Lactobacillus casei Zhang administration exert both preventive and ameliorative effect on oral glucose tolerance AUC in IGT rats but may be via different mechanisms. L. casei Zhang could prevent rats from increased AUC through GLP-2 lowering, while the ameliorative effect in high-fructose-fed post-adolescent rats may be via B. fragilis enriched vitamin K2-dependent osteocalcin mechanism in which AdipoR2, LXR-α and PPAR-γ signaling were involved.

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Abbreviations

AdipoR2:

Adiponectin receptor 2

AUC:

Area under curve

GLP-2:

Glucagon-like peptide-2

HFS:

High fructose syrup

IGT:

Impaired glucose tolerance

IR:

Insulin resistance

LXR-α:

Liver X receptor-α

MDA:

Malondialdehyde

OGTT:

Oral glucose tolerance test

PPAR-γ:

Peroxisome proliferator-activated receptor gamma

SD:

Sprague Dawley

VKORC1:

Vitamin K epoxide reductase complex subunit 1

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Acknowledgments

We thank Dr. Dongmin Liu from Fralin Life Science Institute of Virgina Tech for revising this article. This research was supported by National Natural Science Foundation of China (Grant No. 31025019), the Innovation Team Development of the Ministry of Education of China (Grant No. IRT0967), National Basic Research Program of China (973 Program) (2012CB720802), Hi-Tech Research and Development Program of China (863 Planning, Grant No. 2011AA100901, 2011AA100902) and the Earmarked Fund for Modern Agro-industry Technology Research System (Grant No. nycytx-0501).

Conflict of interest

The authors declare that they have no conflict of interest.

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Author notes

  1. Heping Zhang

    Present address: Key Laboratory of Dairy Biotechnology and Engineering, Education Ministry of P. R. China, Department of Food Science and Engineering, Inner Mongolia Agricultural University, 306 Zhaowudalu Road, Hohhot, 010018, People’s Republic of China

Authors and Affiliations

  1. Key Laboratory of Dairy Biotechnology and Engineering, Education Ministry of P. R. China, Department of Food Science and Engineering, Inner Mongolia Agricultural University, 306 Zhaowudalu Road, Hohhot, 010018, People’s Republic of China

    Yong Zhang, Lifeng Wang, Jiachao Zhang, He Li, Xiao Guo & Jianlin Guo

  2. Department of Biological Science and Engineering, Inner Mongolia Agricultural University, Hohhot, People’s Republic of China

    Yunxu Li

  3. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, People’s Republic of China

    Qiuwen He

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Zhang, Y., Wang, L., Zhang, J. et al. Probiotic Lactobacillus casei Zhang ameliorates high-fructose-induced impaired glucose tolerance in hyperinsulinemia rats. Eur J Nutr 53, 221–232 (2014). https://doi.org/10.1007/s00394-013-0519-5

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