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Protective and therapeutic effects of two novel strains of Lactobacilli on diabetes-associated disorders induced by a high level of fructose

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Abstract

Diabetes is a metabolic disorder described as insufficient secretion of insulin in the pancreas or the inability of the existing insulin to function properly. It poses a greater risk on human health as it is considered the base of several diseases. Thus, this study was designed to evaluate two novel strains of Lactobacillus in handling pancreas disorders. 50 BALB/c male mice were divided into five groups; (a) feeding on normal diet only as control group, (b) given 21% fructose in drinking water as diabetes group, (c) feeding on Lactobacillus rhamnosus strain Pro2 (MT505335.1) plus 21% fructose as LR group, (d) feeding on Lactobacillus plantarum strain Pro1 (MT505334.1) plus 21% fructose as LP group and (e) mixture of two strains plus 21% fructose as Mix group. The serum content of glucose, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) was determined. Pancreases histopathology was examined. Expression of GH, IGF1, and GLP-1 genes was measured in the liver and pancreas by RT-qPCR. Serum content of glucose, ALT, and AST significantly increased in diabetes group, and significantly reduced in (LP) and (Mix) groups compared with control. Pathological changes occurred in the exocrine and endocrine components of the diabetes group pancreas. Besides, islet cells are almost entirely disturbed and acinar cells degenerated. However, in (LP) and (Mix) groups, the pathological changes significantly decreased and became related to the control group. Expression of GH, IGF1, and GLP-1 genes was significantly downregulated in the liver and pancreas of mice given fructose compared with control. Expression of these genes was either significantly upregulated in groups (LP and Mix) or identical to the control group. This study shows that the strain Pro1 (MT505334.1) or a combination of two strains is useful in reducing diabetic risk.

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Data availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Authors and Affiliations

  1. Department of Emergency Medical Services, College of Health Sciences in AlQunfudah, Umm Al-Qura University, Mekkah, Saudi Arabia

    Naif ALSuhaymi

  2. Cell Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Cairo, Egypt

    Ahmed M. Darwish

  3. Genetics and Cytology Department, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Cairo, Egypt

    Abd El-Nasser Khattab

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  1. Naif ALSuhaymi
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Contributions

This study was done in collaboration with all authors. A.K. and A.D. designed this study. N.A., A.D. and A.K. participated in the conduct of the study. N.A. and A.D. analyzed the data. A.D. and A.K. drafted the manuscript. A.D. and N.A. critically revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ahmed M. Darwish.

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The experimental protocol used in the study was approved by the Animal Care and Use Committee of National Research Centre, Egypt. The laboratory experiments and protocols were handled in accordance with the guidelines described by National Research Centre and AlQunfudah, Umm Al-Qura University.

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ALSuhaymi, N., Darwish, A.M. & Khattab, A.EN. Protective and therapeutic effects of two novel strains of Lactobacilli on diabetes-associated disorders induced by a high level of fructose. Mol Biol Rep 48, 4333–4340 (2021). https://doi.org/10.1007/s11033-021-06448-0

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  • DOI: https://doi.org/10.1007/s11033-021-06448-0

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