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Healing of colonic anastomoses after immediate postoperative intraperitoneal administration of oxaliplatin

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Abstract

Aim

The aim of this experimental study was to investigate the effect of intraperitoneal administration of oxaliplatin on the healing of colonic anastomoses when injected immediately after colon resection.

Materials and methods

Thirty male Wistar rats were used. During the operation, the rats were randomized to two groups of 15 rats each. Immediately after colonic anastomoses were performed, the rats were injected intraperitoneally with either 3 ml of 0.9% NaCl solution or oxaliplatin (2.4 mg/kg body weight) depending on their group. All rats were killed on the eighth postoperative day. The anastomoses were examined macroscopically. The anastomotic bursting pressures were recorded, the anastomoses graded histologically, and the hydroxyproline tissue contents determined.

Results

Anastomotic leakage was noted in four rats (26.7%) of the oxaliplatin group, whereas no anastomotic dehiscence was detected among rats of the control group (p = 0.016). The adhesion formation at the anastomotic sites and the inflammatory cell infiltration were significantly higher in the oxaliplatin group than in the control group (p = 0.001). The bursting pressures (p = 0.001), the hydroxyproline tissue content (p = 0.001), the neoangiogenesis (p = 0.033), the fibroblast activity (p = 0.001), and the collagen deposition (p = 0.001) were significantly lower in the oxaliplatin group in comparison to the control group.

Conclusion

The immediate postoperative intraperitoneal administration of oxaliplatin seems to impair healing of colonic anastomoses in rats.

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Authors and Affiliations

  1. 4th Surgical Department, Aristotle University of Thessaloniki, Macedonia, Greece

    Dimitrios Kanellos, Manousos-Georgios Pramateftakis, Haralampos Demetriades, Emmanouil Zacharakis, Stamatios Angelopoulos, Ioannis Mantzoros, Ioannis Kanellos, Kalliopi Despoudi & Haralampos Lazaridis

  2. Histopathology Department, Aristotle University of Thessaloniki, Macedonia, Greece

    Thomas Zaraboukas

  3. Biochemical Laboratory, Aristotle University of Thessaloniki, Macedonia, Greece

    Georgios Koliakos

  4. Microbiological Laboratory, TEI of Thessaloniki, Macedonia, Greece

    Kalliopi Galovatsea

  5. 1 Antheon str, Panorama 55236, Thessaloniki, Macedonia, Greece

    Ioannis Kanellos

Authors
  1. Dimitrios Kanellos
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  2. Manousos-Georgios Pramateftakis
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  3. Haralampos Demetriades
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  4. Emmanouil Zacharakis
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  6. Ioannis Mantzoros
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  8. Kalliopi Despoudi
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  9. Thomas Zaraboukas
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  12. Haralampos Lazaridis
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Correspondence to Ioannis Kanellos.

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Kanellos, D., Pramateftakis, MG., Demetriades, H. et al. Healing of colonic anastomoses after immediate postoperative intraperitoneal administration of oxaliplatin. Int J Colorectal Dis 23, 1185–1191 (2008). https://doi.org/10.1007/s00384-008-0538-5

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  • DOI: https://doi.org/10.1007/s00384-008-0538-5

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