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Validation of IC-VIEW fluorescence videography in a rabbit model of mesenteric ischaemia and reperfusion

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International Journal of Colorectal Disease Aims and scope Submit manuscript

Abstract

Background/Aims

Mortality in mesenteric ischaemia can be reduced by an optimised extent of resection. Up to now, no technique supplementing a surgeon's experience has achieved clinical acceptance. Besides a qualitative interpretation, the new technique of computer-assisted laser-fluorescence videography affords quantification of staining intensities. The aim of this study was to investigate the scientific value of this technique in mesenteric ischaemia in a rabbit model of controlled mesenteric ischaemia and reperfusion.

Methods

We used an established rabbit model of mesenteric ischaemia (group I, n=6) and reperfusion (group II, n=6). In each animal, three loops (each of 10 cm) of the small intestine were clamped (group I, 40 min; group II, 60 and 20 min reperfusion). For further evaluation, all loops were divided into five segments of 2×2 cm (total number of investigated areas, n=180). Measurement of vascular patency was performed by laser-fluorescence videography (pixel intensity per second). As standard, we used radioactive microspheres (impulse per minute per gram). In addition, the extent of ischaemic tissue damage was identified by histological examination. Statistical data were analysed by using regression analysis to define the regression coefficient r.

Results/Findings

Laser-fluorescence videography and the microsphere technique demonstrated a close and linear correlation: ischaemic segments, r=0.90±0.07; reperfusion segments, r=0.85±0.02; overall, r=0.92±0.07. There was no reproducible correlation to cellular damage in histology.

Interpretation/Conclusion

Computer-assisted laser-fluorescence videography is a feasible, reliable, and valid experimental method for the detection of mesenteric blood supply and intestinal microcirculation. Clinical application is conceivable in mesenteric ischaemia and infarction as well as the operative transposition of intestine. As limiting values to identify the irreversible necrosis are not yet defined, further studies have to analyse the clinical impact more precisely.

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Authors and Affiliations

  1. Department of Surgery, Marienhospital, Düsseldorf, Germany

    C. Toens & V. Fernandez

  2. Department of Surgery, Rhenish-Westfalian Technical University, Pauwelsstrasse 30, 52074, Aachen, Germany

    C. J. Krones, J. Grommes, F. Hoelzl, M. Stumpf, U. Klinge & V. Schumpelick

  3. Department of Nuclear Medicine, Rhenish-Westfalian Technical University, Aachen, Germany

    U. Blum

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  1. C. Toens
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  2. C. J. Krones
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  3. U. Blum
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  4. V. Fernandez
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  5. J. Grommes
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  6. F. Hoelzl
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  7. M. Stumpf
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  8. U. Klinge
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Corresponding author

Correspondence to C. J. Krones.

Additional information

C. Toens and C.J. Krones contributed equally to this work.

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Toens, C., Krones, C.J., Blum, U. et al. Validation of IC-VIEW fluorescence videography in a rabbit model of mesenteric ischaemia and reperfusion. Int J Colorectal Dis 21, 332–338 (2006). https://doi.org/10.1007/s00384-005-0017-1

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  • DOI: https://doi.org/10.1007/s00384-005-0017-1

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