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Cytokine/chemokine transcript profiles reflect mucosal inflammation in Crohn’s disease

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International Journal of Colorectal Disease Aims and scope Submit manuscript

Abstract

Background and aims

Immunoregulatory properties of cytokines may contribute to pathological immune reactions in inflammatory bowel disease. There is an urgent need for a simple and dependable means for quantitating inflammatory activity in mucosal biopsies and assessing relapse risk particularly in patients with active Crohn’s disease (CD).

Patients and methods

Cytokine and chemokine transcripts were quantified using real-time PCR in mucosal biopsy specimens from 70 patients with active inflammatory bowel disease (CD, n=45; ulcerative colitis n=25) and 16 patients with specific colitis (ischemic colitis, infectious colitis). Controls were 12 patients with noninflammatory conditions. CD patients with steroid-induced remission (n=20) were followed for up to 12 months.

Results

Compared to not-inflamed mucosa the vast majority of active CD tissue samples expressed significantly elevated transcript levels of IL-1β, IL-8, IL-23, MRP-14, MIP2α, and MMP-1. Moreover, increased cytokine transcript levels were detected in both active ulcerative colitis and specific colitis. Importantly, TNF-α, IFN-γ, CD40L, and IL-23 transcripts increased in active CD only. Transcript levels (MRP-14, IL-8, MMP-1, MIP2α) were correlated with clinical disease activity (CDAI) and endoscopic scoring indices. Medical treatment induced stable remission in 14 of 20 patients which was paralleled by a reduction in increased transcript levels. All six patients without normalization of MIP2α, MRP-14, TNF-α, and IL-1β transcripts developed an early relapse (n=5) or chronic activity (n=1) during follow-up.

Conclusion

Elevated proinflammatory cytokine transcripts in active CD may underlie disease reactivation and chronicity. Real-time PCR quantification is a simple and objective method for grading inflammation of intestinal mucosa and may be useful in identifying patients who would benefit from anti-inflammatory remission maintenance.

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Acknowledgements

The authors thank the nursing staff of the Endoscopy Department for their help in specimen collection. This work was supported in part by a grant from the German Competence Network “Inflammatory Bowel Disease”, AG “Molekulare Marker.”

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Authors and Affiliations

  1. Department of Gastroenterology, Hepatology and Nutritional Medicine, Catholic Clinics Essen-Nord, 45329, Essen, Germany

    Andreas Stallmach

  2. Institute of Immunology, Ruprecht Karl University, 69120, Heidelberg, Germany

    Thomas Giese & Stefan C. Meuer

  3. Department of Internal Medicine II, Saarland University, 66421, Homburg, Germany

    Carsten Schmidt & Bianca Ludwig

  4. Department of Pathology, Saarland University, 66421, Homburg, Germany

    Ina Mueller-Molaian

Authors
  1. Andreas Stallmach
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  2. Thomas Giese
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  3. Carsten Schmidt
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  4. Bianca Ludwig
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  5. Ina Mueller-Molaian
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  6. Stefan C. Meuer
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Corresponding author

Correspondence to Andreas Stallmach.

Additional information

A. Stallmach and T. Giese contributed equally to this work

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Stallmach, A., Giese, T., Schmidt, C. et al. Cytokine/chemokine transcript profiles reflect mucosal inflammation in Crohn’s disease. Int J Colorectal Dis 19, 308–315 (2004). https://doi.org/10.1007/s00384-003-0554-4

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  • DOI: https://doi.org/10.1007/s00384-003-0554-4

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