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Structure and biological activities of lipochitooligosaccharide nodulation signals produced by Bradyrhizobium japonicum USDA 138 under saline and osmotic stress

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Abstract

The establishment of a symbiotic interaction involves a signal exchange between the host legume (flavonoids) and the nitrogen-fixing rhizobia (nodulation factors (NFs)). Likewise, abiotic stress conditions, such as salinity and drought, strongly reduce the nodulation process, possibly affecting also the signal exchange. In this work we characterized the structure and biological activity of NFs produced by Bradyrhizobium japonicum USDA 138 under control, salt, and osmotic stress conditions. This strain is the most widely used in Argentine soybean culture; under control conditions, it produces a mixture of four types of NFs (V(C16:0,MeFuc), V(C18:1,MeFuc), IV(C18:1), and V(C18:1,Ac,MeFuc)). Interestingly, under stress conditions, this strain produces new types of NFs, one common for both stress conditions (V(C16:1,MeFuc)) and another one only present under salt stress (IV(C18:1,MeFuc)). All mixtures of NFs, extracted from control, salt, and osmotic stress conditions, showed biological activity in soybean plants, such as root hair deformation, and the radical application of purified NFs induced systemic differences in dry matter accumulation. The inoculation of soybean with genistein-induced bacteria cultured under both control and stress conditions had a positive effect on the number of nodules formed and in some cases on dry matter accumulation. These responses are not related to changes in chlorophyll fluorescence or greenness index.

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Acknowledgments

This work was supported by grants from Red Iberoamericana de Fertilizantes Biológicos para la Agricultura y el Medio Ambiente (BIOFAG) and Instituto Nacional de Tecnología Agropecuaria (INTA). We thank Esaú Megías Saavedra, Laura Romero Cuadrado, Francisco Javier López Baena, and Francisco Javier Ollero Márquez for the technical assistance.

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Authors and Affiliations

  1. Instituto de Fisiología y Recursos Genéticos Vegetales (IFRGV), INTA, Camino a 60 Cuadras Km 5 ½, X5020 ICA, Córdoba, Argentina

    Nacira Muñoz & Ramiro Lascano

  2. Cátedra de Fisiología Vegetal, FCEFyN, Universidad Nacional de Córdoba, Avda Vélez Sarsfield 299, 5000, Córdoba, Argentina

    Nacira Muñoz & Ramiro Lascano

  3. Servicio de Espectrometría de Masas, Centro de Investigación, Tecnología e Innovación (CITIUS), Universidad de Sevilla, Seville, Spain

    Maria Eugenia Soria-Díaz

  4. Departamento de Química Orgánica, Universidad de Sevilla, Seville, Spain

    Maria Eugenia Soria-Díaz, Rocío Contreras Sánchez-Matamoros & Antonio Gil Serrano

  5. Departamento de Microbiología y Parasitología, Universidad de Sevilla, Seville, Spain

    Hamid Manyani & Manuel Megías

Authors
  1. Nacira Muñoz
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  2. Maria Eugenia Soria-Díaz
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  3. Hamid Manyani
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  4. Rocío Contreras Sánchez-Matamoros
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  5. Antonio Gil Serrano
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  6. Manuel Megías
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  7. Ramiro Lascano
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Corresponding authors

Correspondence to Manuel Megías or Ramiro Lascano.

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Muñoz, N., Soria-Díaz, M.E., Manyani, H. et al. Structure and biological activities of lipochitooligosaccharide nodulation signals produced by Bradyrhizobium japonicum USDA 138 under saline and osmotic stress. Biol Fertil Soils 50, 207–215 (2014). https://doi.org/10.1007/s00374-013-0843-1

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  • DOI: https://doi.org/10.1007/s00374-013-0843-1

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