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Diversity of native rhizobia isolated in south Brazil and their growth promotion effect on white clover (Trifolium repens) and rice (Oryza sativa) plants

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Abstract

In this study, rhizobia strains isolated from white clover (Trifolium repens) root nodules were evaluated in an effort to identify an efficient nitrogen-fixing rhizobia strain that can also improve the growth of rice plants (Oryza sativa). White clover plants were collected from seven sites in south Brazil, and 78 native rhizobia isolates were obtained. The genetic diversity analysis of those isolates was carried out by BOX-polymerase chain reaction. Overall, the native rhizobia isolated showed a high genetic diversity, but when the bacterial isolates from the same site were compared, the diversity was lower. One native rhizobia, POA3 (isolated from the Porto Alegre locality), was able to promote the growth of both plants and is therefore a good candidate for new inoculant formulation. Finally, we can conclude that the community of native rhizobia symbiont of white clover plants in southern Brazil is highly diverse and the growth promotion effect of rhizobia inoculation on rice plants was more pronounced in a poor nutrient substrate condition than in a rich nutrient substrate condition.

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Acknowledgments

This research was supported by grants and fellowships from the International Foundation for Science (IFS, Sweden), Fundação Estadual de Amparo à Pesquisa (FAPERGS, Brazil), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil) and INCT da Fixação Biológica do Nitrogênio (Brazil).

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  1. Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Caixa Postal 15.053, 91501-970, Porto Alegre, RS, Brazil

    Camille E. Granada & Luciane M. P. Passaglia

  2. Fundação Estadual de Pesquisa Agropecuária (FEPAGRO), Rua Gonçalves Dias 570, 90130-060, Porto Alegre, RS, Brazil

    Letícia Arruda, Bruno Brito Lisboa & Luciano K. Vargas

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  1. Camille E. Granada
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  2. Letícia Arruda
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  3. Bruno Brito Lisboa
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  4. Luciane M. P. Passaglia
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  5. Luciano K. Vargas
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Correspondence to Luciane M. P. Passaglia.

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Granada, C.E., Arruda, L., Lisboa, B.B. et al. Diversity of native rhizobia isolated in south Brazil and their growth promotion effect on white clover (Trifolium repens) and rice (Oryza sativa) plants. Biol Fertil Soils 50, 123–132 (2014). https://doi.org/10.1007/s00374-013-0840-4

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