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Gut hormones in relation to body mass and torpor pattern changes during food restriction and re-feeding in the gray mouse lemur

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Abstract

Potential implications of gut hormones in body mass and torpor and behavioral pattern changes induced by an incremental (40 and 80%) calorie restriction (CR) in long-days (LD, summer) and short-days (SD, winter) were investigated in gray mouse lemurs. Only 80% food-deprived LD and SD animals showed a continuous mass loss resulting in a 10 and 15% mass reduction, respectively. Ghrelin levels of all food-deprived groups increased by 2.6-fold on average and remained high after re-feeding while peptide YY (PYY) levels increased by 3.8-fold only in LD animals under 80% CR. In the re-fed SD group, body mass was positively associated with ghrelin and negatively associated with PYY, while no correlations were noted in the re-fed LD animals. Plasma glucagon-like peptide-1 (GLP-1) increased by 2.9-fold only in LD food-restricted mouse lemurs and was negatively associated with the minimal body temperature. No significant correlations were reported in food-deprived SD animals. These results suggest that ghrelin, PYY and GLP-1 may be related to pre-wintering fattening mechanisms and to the modulation of torpor expression, respectively. Such observation clearly warrants further investigations, but it opens an interesting area of research in torpor regulation.

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Abbreviations

AUC:

Area under curve

CR:

Calorie restriction

CRi:

Calorie restriction intensity

GIP:

Glucose-dependant insulinotropic polypeptide

GLP-1:

Glucagon-like peptide 1

LD:

Long-days

LD40:

Animals exposed to long-days under 40% calorie restriction

LD80:

Animals exposed to long-days under 80% calorie restriction

NPY:

Neuropeptide Y

PP:

Peptide P

PYY:

Peptide YY

SD:

Short-days

SD40:

Animals exposed to short-days under 40% calorie restriction

SD80:

Animals exposed to short-days under 80% calorie restriction

Tb:

Body temperature

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Acknowledgments

S. Giroud is supported by an MNRT fellowship. The study was supported by an ATIP from the CNRS (S. Blanc), the Bettencourt Schueller Fondation (Y. Le Maho), the GIS Longévité (S. Blanc) and the ANR Alimentation & Nutrition Humaine (M Perret, S Blanc). This protocol received all ethic authorizations and was conducted under the authorization number 67-223 (CNRS).

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Authors and Affiliations

  1. Mécanismes Adaptatifs et Evolution, UMR 7179 CNRS, Muséum National d’Histoire Naturelle, 4 Avenue du Petit Château, 91800, Brunoy, France

    Sylvain Giroud & Martine Perret

  2. Département d’Ecologie, Physiologie, Ethologie, Institut Pluridisciplinaire Hubert Curien, UMR 7178 CNRS, Université Louis Pasteur, 23 rue Becquerel, 67087, Strasbourg, France

    Sylvain Giroud, Yvon Le Maho, Iman Momken, Caroline Gilbert & Stéphane Blanc

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  1. Sylvain Giroud
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  2. Martine Perret
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Correspondence to Stéphane Blanc.

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Communicated by G. Heldmaier.

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Giroud, S., Perret, M., Le Maho, Y. et al. Gut hormones in relation to body mass and torpor pattern changes during food restriction and re-feeding in the gray mouse lemur. J Comp Physiol B 179, 99–111 (2009). https://doi.org/10.1007/s00360-008-0294-4

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  • DOI: https://doi.org/10.1007/s00360-008-0294-4

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