Abstract
Cellulase genes have been reported not only from fungi, bacteria and plant, but also from some invertebrate animals. Here, two cellulase (endo-β-1,4-glucanase, EC 3.2.1.4) genes, eg27I and eg27II, were cloned from the freshwater snail Ampullaria crossean cDNA using degenerate primers. The nucleotide sequences of the two genes shared 94.5% identity. The open reading frames of both genes consisted of 588 bp, encoding 195 amino acids. Both EG27I and EG27II belong to the glycoside hydrolase family 45, and each lacks a carbohydrate-binding module. The presence of introns demonstrated a eukaryotic origin of the EG27 gene, and, in addition, successful cloning of EG27 cDNA supported endogenous production of EG27 cellulase by Ampullaria crossean. Investigation of the EG27 cDNA from A. crossean will provide further information on GHF45 cellulases.
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Abbreviations
- CBM:
-
Carbohydrate-binding module
- GHF:
-
Glycoside hydrolase family
- CMC:
-
Carboxymethyl cellulose sodium salt
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Acknowledgments
This work was supported by the grants from the National Natural Science Foundation of China (No.30370336), the Major State Basic Research Development Program of China (No.2003CB716006 and No.2004CB719702) and the Creation Foundation from Shanghai Institutes for Life Sciences.
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Communicated by I.D. Hume.
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Guo, R., Ding, M., Zhang, SL. et al. Molecular cloning and characterization of two novel cellulase genes from the mollusc Ampullaria crossean . J Comp Physiol B 178, 209–215 (2008). https://doi.org/10.1007/s00360-007-0214-z
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DOI: https://doi.org/10.1007/s00360-007-0214-z