Abstract
Honeybee workers, Apis mellifera, can reproduce in queenless colonies. The production of queen-like pheromones may be associated with their reproductive activity and induce nestmates to respond by feeding them. Such frequent trophallaxis could supply their protein needs for oogenesis, constituting a social pathway to worker reproduction. However, some individuals can develop ovaries without producing queen pheromones. The consumption of protein-rich pollen could be an alternative solitary pathway for them to satisfy this dietary requirement. In order to investigate the way in which workers obtain proteins for oogenesis, we created orphaned worker groups and determined ovarian and pheromonal development in relation to pollen consumption of selected workers. Individuals that did not consume pollen had significantly more developed ovaries and produced significantly more queen mandibular pheromone than workers that fed directly on pollen. Our results suggest that workers producing queen-like secretions are fed trophallactically. However, reproductive workers that lacked queen pheromones had consumed little or no pollen, suggesting that they also obtained trophallaxis. Although pollen consumption might contribute to sustaining oogenesis, it does not appear to be sufficient. Trophallaxis as a means of obtaining proteins seems to be necessary to attain reproductive status in queenless honeybee colonies.
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Acknowledgments
Appreciation is addressed to Jürgen Liebig for support in the beginning of this study and to Thomas Mürrle for his technical assistance. We thank two anonymous referees for their constructive comments. Financial support was granted by a DAAD fellowship (MS), an Emmy Noether fellowship of the DFG (PN), the Volkswagen Foundation, the National Research Foundation of South Africa and the University of Pretoria (CP, VD and RC). The experiments comply with the “Principles of animal care”, publication No. 86–23, revised 1985 of the National Institute of Health, and with the current laws of South Africa where the experiments were performed.
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Schäfer, M.O., Dietemann, V., Pirk, C.W.W. et al. Individual versus social pathway to honeybee worker reproduction (Apis mellifera): pollen or jelly as protein source for oogenesis?. J Comp Physiol A 192, 761–768 (2006). https://doi.org/10.1007/s00359-006-0112-y
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DOI: https://doi.org/10.1007/s00359-006-0112-y