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The effect of photobleaching on PLIF

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Abstract

Organic dyes commonly used for planar laser-induced fluorescence (PLIF) are susceptible to photobleaching under laser excitation. Previous work has shown that photobleaching can induce significant errors in laser-induced fluorescence measurements made with single-point techniques. This paper presents an analytical model and experimental results that quantify the extent of photobleaching in the more common planar configuration of the technique. Experimental results for two common dyes, fluorescein and Rhodamine 6G, agree well with analytical predictions for typical PLIF parameters. Results indicate that even dyes such as fluorescein that have large photobleaching constants can be used with little error in many PLIF experiments.

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Acknowledgments

This work is supported by the National Science Foundation under CAREER grant No. 0348855 to JPC and a Fannie and John May Hertz Foundation Fellowship to LGL. The authors thank two anonymous reviewers whose suggestions improved this work.

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  1. The University of Colorado, 428 UCB, Boulder, CO, 80309-0428, USA

    L. G. Larsen & J. P. Crimaldi

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  1. L. G. Larsen
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  2. J. P. Crimaldi
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Correspondence to J. P. Crimaldi.

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Larsen, L.G., Crimaldi, J.P. The effect of photobleaching on PLIF. Exp Fluids 41, 803–812 (2006). https://doi.org/10.1007/s00348-006-0205-y

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  • DOI: https://doi.org/10.1007/s00348-006-0205-y

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