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Tränenabfluss

Bedeutung von Muzinen und TFF-Peptiden

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Zusammenfassung

Im Epithel von Tränensack und Tränennasengang kommen außer Basalzellen und hochprismatischen Epithelzellen in großer Menge Becherzellen vor. Ein Hauptsektretionsprodukt der Epithelzellen sind Muzine und TFF-Peptide. Untersuchungen zeigen im Epithel von Tränensack und Tränennasengang, dass die Muzine MUC2, MUC5AC und MUC5B zusammen mit TFF1 von Becherzellen und intraepithelialen Drüsen gebildet werden. Das Muzin MUC7 wird zusammen mit TFF3 von hochprismatischen Epithelzellen gebildet. Bei Patienten mit funktioneller Dakryostenose kommt es zu einer Herabregulation sekretorischer Muzine. Der Nachweis eines breiten Muzinspektrums im Epithel der ableitenden Tränenwege und die Herabregulation sekretorischer Muzine bei der funktionellen Dakryostenose, die mit Epiphora einhergeht, stützt die Annahme, dass Muzine einen wichtigen Faktor für den Tränentransport darstellen. Störungen im Gleichgewicht einzelner Muzine können für die Ausbildung von Dakryostenosen, Dakryozystitiden und Dakryolithen von Bedeutung sein. Die Ergebnisse eröffnen bisher bestehenden Therapiekonzepten bei Erkrankungen des Tränenableitungssystems neue Perspektiven.

Abstract

The epithelial lining of the lacrimal sac and the nasolacrimal duct consists of pseudo-stratified, columnar epithelia rich in goblet cells. Major secretory products of the epithelial cells are mucins together with TFF peptides. Expression and distribution of several mucins and TFF peptides in the human efferent tear ducts was investigated by means of reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. mRNAs for all the mucins investigated, MUC1, MUC2, MUC4, MUC5AC, MUC5B and MUC7, were detected in healthy human lacrimal sacs and nasolacrimal ducts. Both MUC5AC and MUC5B were detected in goblet cells forming intraepithelial mucous glands. MUC7 together with TFF3 occurred only in columnar epithelial cells of the efferent tear duct system. The mucin diversity of the efferent tear ducts could enhance tear transport and antimicrobial defense. The absence of some mucins in non-functioning although patent segments of the lacrimal passage, suggests that mucins ease tear flow through the efferent tear ducts because these conditions are associated with epiphora. Disorders in the balance of single mucins could be of importance with regard to dacryostenosis, dacryocystitis and dacryolith formation.

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Literatur

  1. Arlt v F (1855) Über den Thränenschlauch. Arch Ophthalmol I:135–160

    Google Scholar 

  2. Baughan LW, Robertello FJ, Sarrett DC et al. (2000) Salivary mucin as related to oral Streptococcus mutans in elderly people. Oral Microbiol Immunol 15:10–14

    Article  CAS  Google Scholar 

  3. Becker BB (1992) Tricompartment model of the lacrimal pump mechanism. Ophthalmology 99:7

    PubMed  Google Scholar 

  4. Brockhausen I (1999) Pathways of O-glycan biosynthesis in cancer cells. Biochem Biophys Acta 1473:67–95

    Article  CAS  Google Scholar 

  5. Bumm P, Schauer R (1982) Zur Bedeutung menschlicher Speichel- und Schleimhautmuzine im Bereich der oberen Luft- und Speisewege. HNO 30:414–419

    CAS  Google Scholar 

  6. Chavis RM, Welham RAN, Maisey MN (1978) Quantitative lacrimal scintillography. Arch Ophthalmol 96:2066–2068

    CAS  Google Scholar 

  7. Corfield AP, Carrington SD, Hicks SJ et al. (1997) Ocular mucins: purification, metabolism and functions. Prog Retin Eye Res 16:627–656

    Article  CAS  Google Scholar 

  8. Corfield T (1992) Bacterial sialidases—roles in pathogenicity and nutrition. Glycobiology 2:509–521

    CAS  PubMed  Google Scholar 

  9. Dekker J, Rossen JWA, Büller HA, Einerhand AWC (2002) The MUC familiy: an obituary. Trends Biochem Sci 27:126–131

    Article  CAS  Google Scholar 

  10. Doane MG (1981) Blinking and the mechanics of the lacrimal drainage system. Ophthalmology 88:844–851

    Google Scholar 

  11. Frieberg T (1917) Über die Mechanik der Tränenableitung. Z Augenheilkd 37:42–66, 211–243, 324–366

    Google Scholar 

  12. Hicks SJ, Theodoropoulos G, Carrington SD, Corfield AP (2000) The role of mucins in host-parasite interactions. Part I—protozoan parasites 16:476–481

    Google Scholar 

  13. Hill JC, Bethell W, Smirmaul HJ (1974) Lacrimal drainage—a dynamic evaluation. Can J Ophthalmol 9:411–416

    CAS  Google Scholar 

  14. Hoffmann W, Jagla W (2002) Cell type specific expression of secretory TFF-peptides: colocalization with mucins and synthesis in the brain. Internat Rev Cytol 213:147–181

    CAS  Google Scholar 

  15. Hoffmann W, Jagla W, Wiede A (2001) Molecular medicine of TFF-peptides: from gut to brain. Histol Histopathol 16:319–334

    CAS  PubMed  Google Scholar 

  16. Hurwitz JJ (1996) The nasolacrimal apparatus. In: Hurwitz JJ (ed) The lacrimal system, chapter 4. Lippincott-Raven, Philadelphia, pp 23–29

  17. Jagla W, Wiede A, Hinz M et al. (1999) Secretion of TFF-peptides by human salivary glands. Cell Tissue Res 298:161–166

    Article  CAS  Google Scholar 

  18. Jones LT (1957) Epiphora II. Its relation of the anatomic structures and surgery of the medial cantal region. Am J Ophthalmol 43:203–212

    Google Scholar 

  19. Jones LT (1958) Practical fundamental anatomy and physiology of the eye. Trans Am Acad Ophthalmol Otolaryngol 62:669–678

    CAS  Google Scholar 

  20. Kim YD, Jeon JY, Woo HJ et al. (2002) Interleukin-1beta induces MUC2 gene expression and mucin secretion via activation of PKC-MEK/ERK, and PI3 K in human airway epithelial cells. J Korean Med Sci 17:765–771

    CAS  Google Scholar 

  21. Knop E, Knop N (2001) Lacrimal drainage-associated lymphoid tissue (LDALT): a part of the human mucosal immune system. Invest Ophthalmol Vis Sci 42:566–574

    CAS  PubMed  Google Scholar 

  22. Langer G, Jagla W, Behrens-Baumann W et al. (1999) Secretory peptides TFF1 and TFF3 synthesized in human conjunctival goblet cells. Invest Ophthalmol Vis Sci 40:2220–2224

    CAS  Google Scholar 

  23. Langer G, Walter S, Behrens-Baumann W, Hoffmann W (2001) TFF-Peptide: neue Mukus-assoziierte Sekretionsprodukte der Konjunktiva. Ophthalmologe 98:976–979

    Article  CAS  Google Scholar 

  24. Lee HW, Ahn DH, Crawley SC et al. (2002) Phorbol 12-myristate 13-acetate up-regulates the transcription of MUC2 intestinal mucin via Ras, ERK, and NF-kappa B. J Biol Chem 277:32624–32631

    Article  CAS  Google Scholar 

  25. Lin J, Tsuprun V, Kawano H et al. (2001) Characterization of mucins in human middle ear and Eustachian tube. Am J Physiol Lung Cell Mol Physiol 280:L1157–L1167

    CAS  Google Scholar 

  26. Liu B, Rayment SA, Gyurko C et al. (2000) The recombinant N-terminal region of human salivary mucin MG2 (MUC7) contains a binding domain for oral Streptococci and exhibits candidacidal activity. Biochem J 345:557–564

    Article  CAS  Google Scholar 

  27. Murube del Castillo J (1978) On gravity as one of the impelling forces of lacrimal flow. Asahi Evening News 20th January, pp 51–59

  28. Nagashima K, Araki K (1963) On the lacrimal part of the orbicularis oculi muscle with special reference to the sac dilators. Jap J Ophthalmol 7:220–225

    Google Scholar 

  29. Nielsen PA, Mandel U, Therkildsen MH, Clausen H (1996) Differential expression of human high-molecular-weight salivary mucin (MG1) and low-molecular-weight salivary mucin (MG2). J Dent Res 75:1820–1826

    CAS  Google Scholar 

  30. Nielsen PA, Bennett EP, Wandall HH et al. (1997) Identification of a major human high molecular weight salivary mucin (MG1) as tracheobronchial mucin MUC5B. Glycobiology 7:413–419

    CAS  Google Scholar 

  31. Nik NA, Hurwitz JJ, Chin Sang H (1984) The mechanism of tear flow after DCR and Jones' tube surgery. Arch Ophthalmol 102:1643–1649

    CAS  Google Scholar 

  32. Paulsen F (2002) Schleim—Mucus. Muzine auf dem Gebiet der Hals-, Nasen-, Ohren-Heilkunde. HNO 50:209–219

    CAS  PubMed  Google Scholar 

  33. Paulsen F, Corfield A, Hinz M et al. (2003) Characterization of mucins in human lacrimal sac and nasolacrimal duct. Invest Ophthalmol Vis Sci 44:1807–1813

    Google Scholar 

  34. Paulsen F, Föge M, Thale A et al. (2002) Absorption of lipophilic substances from tear fluid by the epithelium of the nasolacrimal ducts. Invest Ophthalmol Vis Sci 43:3137–3143

    PubMed  Google Scholar 

  35. Paulsen F, Hallmann U, Paulsen J, Thale A (2000) Innervation of the cavernous body of the human efferent tear ducts and function in tear outflow mechanism. J Anat 197:373–381

    Article  PubMed  Google Scholar 

  36. Paulsen F, Hinz M, Schaudig U et al. (2002) TFF-Peptides in the human efferent tear ducts. Invest Ophthalmol Vis Sci 43:3359–3364

    PubMed  Google Scholar 

  37. Paulsen F, Paulsen J, Thale A, Tillmann B (2000) Mucosa-associated lymphoid tissue (MALT) in the human efferent tear ducts. Virch Arch 437:185–189

    Article  CAS  Google Scholar 

  38. Paulsen F, Pufe T, Schaudig U et al. (2001) Detection of natural peptide antibiotics in human nasolacrimal ducts. Invest Ophthalmol Vis Sci 42:2157–2163

    CAS  PubMed  Google Scholar 

  39. Paulsen F, Thale A, Hallmann U et al. (2000) The cavernous body of the human efferent tear ducts—function in tear outflow mechanism. Invest Ophthalmol Vis Sci 41:965–970

    CAS  Google Scholar 

  40. Paulsen F, Thale A, Kohla G et al. (1998) Functional anatomy of human duct epithelium. Anat Embryol 198:1–12

    Article  CAS  Google Scholar 

  41. Paulsen F, Thale A, Maune S, Tillmann B (2001) Primary acquired dacryostenosis—histopathology and pathophysiology. Ophthalmology 108:2329–2336

    Article  CAS  Google Scholar 

  42. Paulsen F, Thale A, Schaudig U (2002) Ableitende Tränenwege und Trockenes Auge. Ophthalmologe 99:566–574

    CAS  Google Scholar 

  43. Perra MT, Serra A, Sirigu P, Turno F (1995) A histochemical and immunohistochemical study of certain defense mechanisms in the human lacrimal sac epithelium. Arch Histol Cytol 58:517–522

    CAS  Google Scholar 

  44. Rohen JW (1953) Morphologische Studien zur Funktion des Lidapparates beim Menschen. Morph Jahrbuch 93:42–97

    Google Scholar 

  45. Rosengren B (1928) Studien über die Tränenableitung beim Menschen und einigen Säugetieren. Svenska Läkare Sällskapets Handlingar 54:41–117

    Google Scholar 

  46. Schauer R (1997) Enzyme als Werkzeuge für die Analyse der Struktur und Funktion von sialylierten Glykokonjugaten. BIO-forum 20:541–544

    Google Scholar 

  47. Schirmer O (1903) Studien zur Physiologie und Pathologie der Tränenabsonderung und Tränenabfuhr. Arch Ophthalmol 56:197–212

    Google Scholar 

  48. Sirigu P, Maxia C, Puxeddu R et al. (2000) The presence of a local immune system in the upper blind and lower part of the human nasolacrimal duct. Arch Histol Cytol 63:431–439

    CAS  Google Scholar 

  49. Thale A, Paulsen F, Rochels R, Tillmann B (1998) Functional anatomy of human efferent tear ducts—a new theory of tear outflow. Graefes Arch Clin Exp Ophthalmol 236:674–678

    Article  CAS  Google Scholar 

  50. Theodoropoulos G, Hicks SJ, Corfield AP et al. (2001) The role of mucins in host-parasite interactions: part II—helminth parasites. Trends Parasitol 17:130–135

    Article  CAS  Google Scholar 

  51. Thim L, Madsen F, Poulsen SS (2002) Effect of trefoil factors on the viscoelastic properties of mucus gels. Eur J Clin Invest 32:519–527

    Article  CAS  Google Scholar 

  52. Tomasetto C, Masson R, Linaires JL et al. (2000) pS2/TFF1 interacts directly with the VWFC cysteine-rich domains of mucins. Gastroenterology 118:70–80

    CAS  Google Scholar 

  53. Van Klinken BJW, Dekker J, Büller HA, Einerhand AWC (1995) Mucin gene structure and expression: protection vs. adhesion. Am J Physiol 269:G613–627

    PubMed  Google Scholar 

  54. Wilson G, Merril R (1976) The lacrimal drainage system: Pressure changes in the canaliculus. Am J Optom Physiol Optics 53:55–59

    CAS  Google Scholar 

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Danksagung

Die Arbeiten wurden von der Deutschen Forschungsgemeinschaft (Pa738/1–2 und 1–3 an F.P.), dem National Eye Research Center (NERC grant 2000/013 an M.B.), dem Fond der Chemischen Industrie (0163615 sowie 0500058 an W.H.) und der AG Trockenes Auge im BVA unterstützt.

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Authors and Affiliations

  1. Anatomisches Institut, Christian-Albrechts-Universität, Kiel

    F. Paulsen

  2. Medicine Laboratories, University of Bristol

    A. Corfield

  3. Institut für Medizinische Chemie, Otto-von-Guericke-Universität Magdeburg

    M. Hinz & W. Hoffmann

  4. Klinik für Ophthalmologie, Universitätskrankenhaus Hamburg-Eppendorf

    U. Schaudig

  5. Klinik für Opthalmologie, Christian-Albrechts-Universität, Kiel

    A. Thale

  6. Division of Ophthalmology, University of Bristol

    M. Berry

  7. Anatomisches Institut der Christian-Albrechts-Universität, Olshausenstraße 40, 24098 , Kiel

    F. Paulsen

Authors
  1. F. Paulsen
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  2. A. Corfield
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  3. M. Hinz
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  4. W. Hoffmann
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  5. U. Schaudig
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  6. A. Thale
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  7. M. Berry
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Correspondence to F. Paulsen.

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Teile der Untersuchungsergebnisse wurden als Kurzvortrag auf der 100. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft 2002 vorgestellt.

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Paulsen, F., Corfield, A., Hinz, M. et al. Tränenabfluss. Ophthalmologe 101, 19–24 (2004). https://doi.org/10.1007/s00347-003-0842-y

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