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Erythropoietin producing cells for potential cell therapy

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Abstract

Introduction

Anemia is an inevitable outcome of chronic renal failure due to the kidney’s decreased ability to produce erythropoietin (EPO). We examined the feasibility of isolating and expanding EPO-producing cells for cell-based therapy.

Materials and methods

Renal cells from 7- to 10-day-old mice were culture-expanded. The cells at each subculture stage were characterized for EPO expression, using immunocytochemistry, FACS, and Western Blot analysis, with EPO-specific antibodies. To assess the levels of EPO expression, cells incubated under normoxic and hypoxic conditions were analyzed.

Results

Immunocytochemical analysis of the cultured renal cells expressed EPO at each subculture stage (P1–P3). Western Blot analysis of the detergent-solubilized cell extracts detected EPO (34 kDa) protein in the kidney cells of all passages tested.

Conclusion

These results demonstrate that EPO-producing renal cells can be grown and expanded in culture. The cells stably expressed EPO at multiple subculture stages and they are able to form tissue in vivo. This study shows that EPO-producing cells may be used as a potential treatment option for anemia caused by chronic renal failure.

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Acknowledgments

This work was supported by Tengion, Inc., through a Sponsored Research Agreement. The authors would like to thank Dr. Jennifer Olson for editorial assistance.

Conflict of interest statement

James Yoo and Anthony Atala serve as consultants for Tengion, Inc.

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Authors and Affiliations

  1. Wake Forest Institute for Regenerative Medicine and Department of Urology, Wake Forest University Health Sciences, Medical Center Boulevard, Winston-Salem, NC, 27157, USA

    Tamer Aboushwareb, Fernanda Egydio, Lauren Straker, Kenneth Gyabaah, Anthony Atala & James J. Yoo

Authors
  1. Tamer Aboushwareb
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  2. Fernanda Egydio
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  3. Lauren Straker
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  4. Kenneth Gyabaah
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  5. Anthony Atala
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  6. James J. Yoo
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Corresponding author

Correspondence to James J. Yoo.

Additional information

Tamer Aboushwareb and Fernanda Egydio contributed equally to this article.

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Aboushwareb, T., Egydio, F., Straker, L. et al. Erythropoietin producing cells for potential cell therapy. World J Urol 26, 295–300 (2008). https://doi.org/10.1007/s00345-008-0301-0

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  • DOI: https://doi.org/10.1007/s00345-008-0301-0

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