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Metabolomics Analysis Reveals Major Differential Metabolites and Metabolic Alterations in Tea Plant Leaves (Camellia sinensis L.) Under Different Fluorine Conditions

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Abstract

Tea plant (Camellia sinensis L.) is capable of accumulating a large amount of fluorine (F) in leaves without showing toxicity symptoms and thus offers a good model for exploring F tolerance mechanisms. Here, gas chromatography time-of-flight mass spectrometry (GC-TOF–MS) was used to investigate metabolic changes in leaves of tea seedlings under control (0 mM), low F (0.2 mM) and high F (0.8 mM) conditions. Differentially changed metabolites such as galacturonic acid, lactose, fructose, malic acid, alanine were identified by the comparison among the three F treatment groups. A pathway map depicted based on the KEGG database reflected the involvement of pectin biosynthesis metabolism in F stress response. The gene expression and enzyme activity of key enzymes involved in pectin biosynthesis pathway and the content of pectic polysaccharides were increased by exogenous F treatments, indicating the promotion effect of F on the pectin biosynthesis. Pectin was also immunochemically stained in vivo using monoclonal antibody (2F4), which confirmed the increment. The increased pectin might contribute to combining the exogenous F in tea leaves. This research provided some novel insights into further research on F detoxification of plants.

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Acknowledgements

This work was financially supported by grant from National Natural Science Foundation of China (No. 31470691).

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Authors and Affiliations

  1. Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China

    Jinlei Luo, Kai Hu, Fengfeng Qu, Dejiang Ni, Haojie Zhang, Siyi Liu & Yuqiong Chen

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  1. Jinlei Luo
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  2. Kai Hu
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  3. Fengfeng Qu
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Correspondence to Yuqiong Chen.

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Luo, J., Hu, K., Qu, F. et al. Metabolomics Analysis Reveals Major Differential Metabolites and Metabolic Alterations in Tea Plant Leaves (Camellia sinensis L.) Under Different Fluorine Conditions. J Plant Growth Regul 40, 798–810 (2021). https://doi.org/10.1007/s00344-020-10141-0

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  • DOI: https://doi.org/10.1007/s00344-020-10141-0

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