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The preparation and antioxidant activity of glucosamine sulfate

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Chinese Journal of Oceanology and Limnology Aims and scope Submit manuscript

Abstract

Glucosamine sulfate was prepared from glucosamine hydrochloride that was produced by acidic hydrolysis of chitin by ion-exchange method. Optical rotation and elemental analysis characterized the degree of its purity. In addition, the antioxidant potency of chitosan derivative-glucosamine sulfate was investigated in various established in vitro systems, such as superoxide (O −2 )/hydroxyl (·OH) radicals scavenging, reducing power, iron ion chelating. The following results are obtained: first, glucosamine sulfate had pronounced scavenging effect on superoxide radical. For example the O −2 scavenging activity of glucosamine sulfate was 92.11% at 0.8 mg/mL. Second, the ·OH scavenging activity of glucosamine sulfate was also strong, and was about 50% at 3.2 mg/mL. Third, the reducing power of glucosamine sulfate was more pronounced. The reducing power of glucosamine sulfate was 0.643 at 0.75 mg/mL. However, its potency for ferrous ion chelating was weak. Furthermore, except for ferrous ion chelating potency, the scavenging rate of radical and reducing power of glucosamine sulfate were concentration-dependent and increased with their increasing concentrations, but its ferrous ion chelating potency decreased with the increasing concentration. The multiple antioxidant activities of glucosamine sulfate were evidents of reducing power and superoxide/hydroxyl radicals scavenging ability. These in vitro results suggest the possibility that glucosamine sulfate could be used effectively as an ingredient in health or functional food, to alleviate oxidative stress.

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Authors and Affiliations

  1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Ronge Xing  (邢荣娥), Song Liu  (刘松), Lin Wang  (王琳), Shengbao Cai  (蔡圣宝), Huahua Yu  (于华华), Jinhua Feng  (冯金华) & Pengcheng Li  (李鹏程)

  2. Graduate School of Chinese Academy of Sciences, Beijing, 100039, China

    Lin Wang  (王琳), Shengbao Cai  (蔡圣宝) & Jinhua Feng  (冯金华)

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  1. Ronge Xing  (邢荣娥)
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  2. Song Liu  (刘松)
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  3. Lin Wang  (王琳)
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  4. Shengbao Cai  (蔡圣宝)
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  5. Huahua Yu  (于华华)
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  6. Jinhua Feng  (冯金华)
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  7. Pengcheng Li  (李鹏程)
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Correspondence to Ronge Xing  (邢荣娥) or Pengcheng Li  (李鹏程).

Additional information

Supported by the Talent Program of Chinese Academy of Sciences (Dongbei Zhi Chun) and Award Foundation of Scientific Research for Excellent Young and Middle-Age Scientist of Shandong Province (No. 2007BS07002)

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Xing, R., Liu, S., Wang, L. et al. The preparation and antioxidant activity of glucosamine sulfate. Chin. J. Ocean. Limnol. 27, 283–287 (2009). https://doi.org/10.1007/s00343-009-9135-x

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  • DOI: https://doi.org/10.1007/s00343-009-9135-x

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