Abstract
Acidic oligosaccharide sugar chain (AOSC), a D-mannuronic acid oligosaccharide, derived from brown algae polysaccharide, has been completed Phase I clinical trial in China as an anti-Alzheimer’s Disease (AD) drug candidate. The identification of AOSC-binding protein(s) in neurons is very important for understanding its action mechanism. To determine the binding protein(s) of AOSC in neurons mediating its anti-AD activities, confocal microscopy, affinity chromatography, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis were used. Confocal microscopy analysis shows that AOSC binds to SH-SY5Y cells in concentration-, time-, and temperature-dependent fashions. The AOSC binding proteins were purified by affinity chromatography and identified by LC-MS/MS analysis. The results showed that there are 349 proteins binding AOSC, including clathrin, adaptor protein-2 (AP-2) and amyloid precursor protein (APP). These results suggest that the binding/entrance of AOSC to neurons is probably responsible for anti-AD activities.
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Supported by National Basic Research Program of China (973 Program) (No. 2003CB716400); Natural Science Foundation of China for Distinguished Young Scholars (No. 30725046); Natural Science Foundation of China (No. 30472029, No. 30772612) and Chinese High technology Research and Development Program of China(863 Program) (No. 2006AA090501)
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Liu, M., Nie, Q., Xin, X. et al. Identification of AOSC-binding proteins in neurons. Chin. J. Ocean. Limnol. 26, 394–399 (2008). https://doi.org/10.1007/s00343-008-0394-8
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DOI: https://doi.org/10.1007/s00343-008-0394-8