Abstract
In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid (IMI) to the gill cell line of flounder (FG) that collected in the gill of Paralichthys olivaceus, was examined by 3 widely used endpoint bioassays: NR (neutral red), MTT (3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) and TCP (total cell protein). The result shows that the IMI increased at concentrations ≥0.5 μg/ml. The IC50 value of NR. MTT, and TCP was 41.86, 38.46, and 39.08 μg/ml, respectively. The ultrastructural observation revealed that the mitochondria of the cells exposed to 60 μg/ml IMI for 48 h were severely damaged, swollen or disrupted, while their nuclei and rough endoplasmic reticulum (RER) remained normal. This would suggest that the mitochondria are probably the primary target of IMI.
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Supported by the Ministry of Science and Technology (MOST) of China (No. 2001AA649040) and the Scientific-Research Fund of Qingdao University of Science and Technology (No.0022168)
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Su, F., Zhang, S., Li, H. et al. In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid to gill cell line of flounder Paralichthy olivaceus. Chin. J. Ocean. Limnol. 25, 209–214 (2007). https://doi.org/10.1007/s00343-007-0209-3
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DOI: https://doi.org/10.1007/s00343-007-0209-3