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Organization and expression of bovine TSPY

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Abstract

We have isolated genomic sequences as well as transcripts from the bovine homolog of the human testis-specific protein, Y-encoded, TSPY which—in both species—is located on the Y Chromosome (Chr), organized as a gene family with a variable number of members, and expressed exclusively in the testis. 1266 bp of bovine TSPY specific sequence have been isolated from a testis cDNA library, by RT-PCR analyses and by Rapid Amplification of cDNA Ends (RACE). A bovine TSPY gene 4 is organized in seven exons, and transcripts are polyadenylated at various 3′ ends. Consensus polyadenylation signals AA UUAAA are missing. Microheterogeneous sequence variation is found between TSPY family members. In addition, homologies to other Y-located repeated sequence families, BRY, have been discovered; these sequences are presumably derived from ancient members of the TSPY cluster, now forming a separate, probably nonfunctional subfamily. Bovine TSPY is subject to differential splicing. In the adult, it is expressed in early germ-cell stages, and expression could also be detected in fetal testis. Comparison with the human homolog shows the highest degree of similarity in the coding regions of exons 2, 3, and 4, which are also precisely conserved regarding their length.

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Author notes

  1. Frank Schnieders

    Present address: Max Delbrück-Center für molekulare Medizin, Max-Planck-Gesellschaft und Humboldt Universität, D-13125, Berlin, Germany

Authors and Affiliations

  1. Institut für Humangenetik, Medizinische Hochschule Hannover, D-30623, Hannover, Germany

    Tanja Vogel, Frank Dechend, Christian Jung, Jörg Schmidtke & Frank Schnieders

  2. Tierklinik für Fortpflanzung, Freie Universität Berlin, D-14163, Berlin, Germany

    Eberhard Manz

  3. Institut für Humangenetik, Otto-von-Guericke-Universität, D-39104, Magdeburg, Germany

    Sybille Jakubiczka

  4. Zentrum für molekulare Neurobiologie Hamburg, Universität Hamburg, D-20246, Hamburg, Germany

    Susanne Fehr

Authors
  1. Tanja Vogel
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  2. Frank Dechend
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  3. Eberhard Manz
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  4. Christian Jung
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  5. Sybille Jakubiczka
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  6. Susanne Fehr
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  7. Jörg Schmidtke
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  8. Frank Schnieders
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Additional information

The nucleotide sequence data reported in this paper have been submitted to GenBank and have been assigned the accession numbers U75888, U75889, U75890, U75891, U75892, U75893, U75894, U75895, U75896.

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Vogel, T., Dechend, F., Manz, E. et al. Organization and expression of bovine TSPY . Mammalian Genome 8, 491–496 (1997). https://doi.org/10.1007/s003359900482

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  • DOI: https://doi.org/10.1007/s003359900482

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