Abstract
Two lambda phage and 66 cosmids containing informative porcine microsatellites were assigned to 17 of 18 porcine autosomes and the X Chromosome (Chr) by fluorescence in situ hybridization (FISH). These assignments provide additional physically anchored markers to integrate the porcine physical and genetic maps.
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Alexander, L.J., Rohrer, G.A., Stone, R.T., Beattie, C.W. (1995a). Porcine SINE associated microsatellite markers: evidence for new artiodactyl SINEs. Mamm. Genome 6, 464–468.
Alexander, L.J., Rohrer, G.A., Beattie, C.W. (1996). Cloning and characterization of 414 polymorphic porcine microsatellites. Anim. Gent, in press.
Archibald, A.L., Haley, C.S., Brown, J.F., Couperwhite, S., McQueen, H.A., Nicholson, D., Coppieters, W., Van de Weghe, A., Stratil, A., Winterø, A.K., Fredholm, M., Larsen, N.J., Nielsen, V.H., Milan, D., Woloszyn, N., Robic, A., Dalens, M., Riquet, J., Gellin, J., Caritez, J.-C., Burgaud, G., Olliver, L., Bidanel, J.-P., Vaiman, M., Renard, C., Geldermann, H., Davoli, R., Ruyter, D., Verstege, E.J.M., Groenen, M.A.M., Davies, W., Hoyheim, B., Keiserud, A., Andersson, L., Ellegren, H., Johannson, M., Marklund, L., Miller, J.R., Anderson Dear, D.V., Signer, E., Jeffreys, A.J., Moran, C., Le Tissier, P., Muladno, Rothschild, M.F., Tuggle, C.K., Vaske, D., Helm, J., Liu, H.-C., Rahman, A., Yu, T.-P., Larson, R.G., Schmitz, C.B. (1995). The PiGMaP consortium linkage map of the pig (Sus scrofa). Mamm. Genome 6, 157–175.
Beattie, C.W. (1994). Livestock genome maps. Trends Genet. 10, 334–338.
Beckmann, J.S., Weber, J.L. (1992). Survey of human and rat microsatellites. Genomics 12, 627–631.
Browne, D.L., Litt, M. (1992). Characterization of (CA)n microsatellites with degenerate sequencing primers. Nucleic Acids Res. 20, 141.
Cohen, D., Chumakov, I., Weissenbach, J. (1993). A first generation physical map of the human genome. Nature 336, 698–701.
Ellegren, H., Chowdhary, B.P., Johansson, M., Marklund, L., Fredholm, M., Gustavsson, I., Andersson, L. (1994a). A primary linkage map of the porcine genome reveals a low rate of genetic recombination. Genetics 137, 1089–1100.
Ellegren, H., Chowdhary, B.P., Johansson, M., Andersson, L. (1994b). Integrating the porcine physical and linkage map using cosmid-derived markers. Anim. Genet. 25, 155–164.
Green, P., Falls, K., Crooks, S. (1990). Documentation for CRI-MAP, version 2.4. (St. Louis, Mo.: Washington University School of Medicine).
Gustavsson, I. (1988). Standard karyotype of the domestic pig. Committee for the Standardized Karotype of the Domestic Pig. Hereditas 109, 151–157.
Hawkins, G.A., Solinas-Toldo, S., Bishop, M.D., Kappes, S.M., Fries, R., Beattie, C.W. (1995). Physical and linkage mapping of the bovine genome with cosmids. Mamm. Genome 6, 249–254.
Israel, D.I. (1993). A PCR-based method for high stringency screening of DNA libraries. Nucleic Acids Res. 21, 2627–2631.
Lemieux, N., Dutrillaux, B., Viegas-Pequignot, E. (1992). A simple method for simultaneous R- or G-banding and fluorescence in situ hybridization of small single-copy genes. Cytogenet. Cell Genet. 59, 311–312.
Lichter, P., Cremer, T., Bordon, J., Manuelidis, L., Ward, D.C. (1988). Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Human Genet. 80, 224–234.
Litt, M., Luty, J.A. (1989). A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat with the cardiac muscle actin gene. Am. J. Hum. Genet. 44, 397–401.
Matise, T.C., Perlin, M., Chakravarti, A. (1994). Automated construction of genetic linkage maps using an expert system (MultiMap): a human genome linkage map. Nature Genet. 6, 384–390.
Murray, J.C., Buetow, K.H., Hudson, T., Weber, J.L., Scherpbier-Heddema, T. (1992). A comprehensive genetic linkage map of the human genome. Science 258, 67–86.
O’Brien, S.J., Womack, J.E., Lyons, L.A., Moore, K.J., Jenkins, N.A., Copeland, N.G. (1993). Anchor reference loci for comparative genome mapping in mammals. Nature Genet. 3, 103–112.
Rettenberger, G., Klett, C, Zenchner, U., Kuntz, J., Vogel, W., Hameister, H. (1995a). Visualization of the conservation of synteny between humans and pigs by heterologous chromosomal painting. Genomics 26, 372–378.
Rettenberger, G., Bruch, J., Beattie, C.W., Moran, C., Fries, R., Hameister, H. (1995b). Chromosomal assignments of seventeen porcine microsatellites and genes by use of a somatic cell hybrid mapping panel. Anim. Genet. 26, 269–273.
Rohrer, G.A., Alexander, L.J., Keele, J.W., Smith, T.P., Beattie, C.W. (1994). A microsatellite linkage map of the porcine genome. Genetics 136, 231–245.
Sambrook, J., Fritsch, E.F., Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd ed. (Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press).
Singer, D.S., Parent, L.J., Ehrlich, R. (1987). Identification and DNA sequence of an interdispersed repetitive DNA element in the genome of a miniature swine. Nucleic Acids Res. 15, 2780.
Smith, T.L.P., Rohrer, G.A., Alexander, L.J., Troyer, D.L., Kirby-Dobbels, K.R., Jancen, M.A., Cornwell, D.L., Louis, CF., Schook, L.B., Beattie, C.W. (1995). Directed integration of the physical and genetic linkage maps of swine chromosome 7 reveals the SLA spans the centromere. Genome Res. 5, 259–276.
Solinas-Tolda, S., Fries, R., Steffen, P., Neibergs, H.L., Barendse, W., Womack, J.E., Hetzel, D.J.S., Stranzinger, G. (1993). Physically mapped, cosmid-derived microsatellite markers as anchor loci on bovine chromosomes. Mamm. Genome 4, 720–727.
Thomsen, P.D., Hoyheim, B. (1994). S0306-a molecular marker at pig chromosome 18q 24. Anim. Genet. 25, 377.
Troyer, D.L., Goad, D.W., Xie, H., Rohrer, G.A., Alexander, L.J., Beattie, C.W. (1994). Use of direct in situ single copy (DISC)PCR to physically map five porcine microsatellites. Cytogenet. Cell Genet. 67, 199–204.
Weber, J.L., May, P.E. (1989). Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am. J. Hum. Genet. 44, 388–396.
Yerle, M., Lahbib-Mansais, Y., Mellink, C, Goureau, A., Pinton, P., Echard, G., Gellin, J., Zijlstra, C., De Hann, N., Bosma, A.A., Chowdhary, B., Gu, F., Gustavsson, I., Thomsen, P.D., Christensen, K., Rettenberger, G., Hameister, H., Schmitz, A., Chaput, B., Freiet, G. (1995). The PiGMaP consortium cytogenetic map of the domestic pig (Sus scrofa domestica). Mamm. Genome 6, 176–186.
Zahn, L.M., Kwiatkowski, D.J. (1995). A 37-marker PCR-based genetic linkage map of human chromosome 9: observations on mutations and positive interference. Genomics 28, 140–146.
Zhang, Q., Wrana, J.L., Sodek, J. (1992). Characterization of the promoter region of the porcine OPN (osteopontin secreted phosphoprotein 1) gene: identification of positive and negative regulatory elements and a ‘silent’ second promoter. Eur. J. Biochem. 207, 649–659.
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Alexander, L.J., Troyer, D.L., Rohrer, G.A. et al. Physical assignments of 68 porcine cosmid and lambda clones containing polymorphic microsatellites. Mammalian Genome 7, 368–372 (1996). https://doi.org/10.1007/s003359900106
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DOI: https://doi.org/10.1007/s003359900106