Abstract
To identify physical and genetic anchor loci on bovine chromosomes, 13 cosmids, obtained after the screening of partial bovine cosmid libraries with the (CA)n microsatellite motif, were mapped by fluorescence in situ hybridization (FISH). Eleven cosmid probes yielded a specific signal on one of the bovine chromosomes and identified the following loci: D5S2, D5S3, D6S3, D8S1, D11S5, D13S1, D16S5, D17S2, D19S2, D19S3, D21S8. Two cosmids produced centromeric signals on many chromosomes. The microsatellite-containing regions were subcloned and sequenced. The sequence information revealed that the two centromeric cosmids were derived from bovine satellites 1.723 and 1.709, respectively. A cosmid located in the subtelomeric region of Chromosome (Chr) 17 (D17S2) had features of a chromosome-specific satellite. Primers were designed for eight of the nonsatellite cosmids, and seven of these microsatellites were polymorphic with between three and eight alleles on a set of outbred reference families. The polymorphic and chromosomally mapped loci can now be used to physically anchor other bovine polymorphic markers by linkage analysis. The microsatellite primers were also applied to DNA samples of a previously characterized panel of somatic hybrid cell lines, allowing the assignment of seven microsatellite loci to defined syntenic groups. These assignments confirmed earlier mapping results, revealed a probable case of false synteny, and placed two formerly unassigned syntenic groups on specific chromosomes.
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Barendse, W., Armitage, S.M., Ryan, A.M., Day, D., Moore, S.S., Clayton, D., Georges, M., Womack, J.E., Hetzel, J. (1993). A genetic map of DNA loci on bovine chromosome 1. Genomics, in press.
Boyle, A.L., Ward, D.C. (1992). Isolation and initial characterization of a large repeat sequence element specific to mouse chromosome 8. Genomics 12, 517–525.
Chapman, V.M., Nadeau, J.H. (1992). The mouse genome: an overview. Curr. Opin. Genet. Dev. 2, 406–411.
Chevalet, C., Corpet, F. (1986). Statistical decision rules concerning synteny or independence between markers. Cytogenet. Cell Gent. 43, 132–139.
Evans, G.A., Lewis, K., Rothenberg, B.E. (1989). High efficiency vectors for cosmid microcloning and genomic analysis. Gene 79, 9–20.
Farr, C.J., Goodfellow, P.N. (1992). Hidden messages in genetic maps. Science 258, 49.
Fries, R. (1993). Mapping the bovine genome: methodological aspects and strategy. Anim. Genet. 24, 111–116.
Fries, R., Threadgill, D.W., Hediger, R., Gunawardana, A., Blessing, M., Jorcano, J.L., Stranzinger, G., Womack, J.E. (1991). Mapping of bovine cytokeratin sequences to four different sites on three chromosomes. Cytogenet. Cell Genet 57, 135–141.
Fries, R., Eggen, A., Womack, J.E. (1993). The bovine genome map. Mammalian Genome 4, 405–428.
Hayes, H., Petit, E. (1993). Mapping of the beta-lactoglobulin gene and of an immunoglobulin M heavy chain-like sequence to homologous cattle, sheep, and goat chromosomes. Mammalian Genome 4, 207–210.
Hearne, C.M., Ghosh, S., Todd, J.A. (1992). Microsatellites for linkage analysis of genetic traits. Trends Genet. 8, 288–294.
Heery, D.M., Gannon, F., Powell, R. (1990). A simple method for subcloning DNA fragments from gel slices. Trends Genet. 6, 173.
ISCNDA (1989). International system for cytogenetic nomenclature of domestic animals. Cytogenet. Cell Genet. 53, 65–79.
Johnson, G.E., Davidson, R.S., McNamee, K.C., Russell, G., Goodwin, R.D., Holborow, E.J. (1982). Fading of immunofluorescence during microscopy: a study of the phenomenon and its remedy. J. Immunol. Methods 55, 231–242.
Johnson, S.E., Barendse, W., Hetzel, D.J.S. (1993). The gammafibrinogen gene (FGG) maps to bovine chromosome 17 in both cattle and sheep. Cytogenet. Cell Genet. 62, 176–180.
Kretz, K.A., Carson, G.S., O'Brien, J.S. (1989). Direct sequencing rom low-melt agarose with Sequenase®. Nucleic Acids Res. 17, 5864.
Lichter, P., Tang, C.-J., Call, K., Hermanson, G., Evans, G.A., Housman, D., Ward, D.C. (1990). High resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science 247, 64–69
Litt, M., Luty, J.A. (1989). A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. Am. J. Hum. Genet. 44, 397–401.
Macaya, G., Cortadas, J., Bernardi, G. (1978). An analysis of the bovine genome by density-gradient centrifugation. Eur. J. Biochem. 84, 179–188.
O'Brien, S.J. (1991). Mammalian genome mapping: lessons and prospects. Curr. Opin. Genet. Dev. 1, 105–111.
O'Brien, S.J., Womack, J.E., Lyons, L.A., Moore, K.J., Jenkins, N.A., Copeland, N.G. (1993). Anchored reference loci for comparative genome mapping in mammals. Nature Genet. 3, 103–112.
Olson, M., Hood, L., Cantor, C., Botstein, D. (1989). A common language for physical mapping of the human genome. Science 245, 1434–1435.
Plucienniczak, G., Skowronski, J., Plucienniczak, A., Jaworski, J. (1985). Nucleotide sequence of bovine 1.723 satellite DNA. Z. Naturforsch. 40, 242–246.
Saiki, R.K., Gelfand, D.H., Stoffel, S., Scharf, S.J., Higuchi, R., Horn, G.T., Mullis, K.B., Erlich, H.A. (1988). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487–491.
Saris, P.E.J., Paulin, L.G., Uhlén, M. (1990). Direct amplification of DNA from colonies of Bacillus subtilis and Escherichia coli by the polymerase chain reaction. J. Microbiol. Methods 11, 121–126.
Skowronski, J., Plucienniczak, A., Bednarek, A., Jaworski, J. (1984). Bovine 1.709 satellite: recombination hotspots and dispersed repeated sequences. J. Mol. Biol. 177, 399–416.
Stallings, R.L., Ford, A.F., Nelson, D., Torney, D.C., Hildebrand, C.E., Moyzis, R.K. (1991). Evolution and distribution of (GT)n repetitive sequences in mammalian genomes. Genomics 10, 807–815.
Steffen, P., Eggen, A., Fries, R. (1991). Method for isolating microsatellites by the consequent use of PCR. Cytogenet. Cell Genet. (abstr.) 58, 2153–2154.
Steffen, P., Eggen, A., Dietz, A.B., Womack, J.E., Stranzinger, G., Fries, R. (1993). Isolation and mapping of polymorphic microsatellites in cattle. Anim. Genet. 24, 121–124.
Vaiman, D., Osta, D., Mercier, D., Grohs, C., Levéziel, H. (1992). Characterization of five new bovine dinucleotide repeats. Anim. Genet. 23, 537–541.
Weber, J.L. (1990). Informativeness of human (dC-dA)n · (dG-dT)n polymorphisms. Genomics 7, 524–530.
Weber, J.L., May, P.E. (1989). Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am. J. Hum. Genet. 44, 388–396.
Weissenbach, J., Gyapay, G., Dib, C., Vignal, A., Morissette, J., Millasseau, P., Vaysseix, G., Lathrop, M. (1992). A second-generation linkage map of the human genome. Nature 359, 794–801.
Womack, J.E. (1987). Genetic engineering in agriculture: animal genetics and development. Trends Genet. 3, 65–68.
Womack, J.E., Moll, Y.D. (1986). Gene map of the cow: conservation of linkage with mouse and man. J. Hered. 77, 2–7.
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Toldo, S.S., Fries, R., Steffen, P. et al. Physically mapped, cosmid-derived microsatellite markers as anchor loci on bovine chromosomes. Mammalian Genome 4, 720–727 (1993). https://doi.org/10.1007/BF00357796
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DOI: https://doi.org/10.1007/BF00357796