Abstract
We have isolated a clone from a C57BL/6 genomic library that contains both part of the Y Chromosome-specific 8.7 kbp MuRVY genome (Hutchinson and Eicher, J. Virol. 63, 4043, 1989) and a full-length 8.3 kbp Intracistemal A Particle genome (IAPE-Y), in a tail-to-tail organization. Although IAPs are encoded by a disperse multigene family (1000 copies per haploid genome), we present evidence that a significant proportion of the IAP-related sequences are present on the Y Chromosome (Chr) and that a >25 kbp genomic sequence, which contains the two proviral genomes, has been amplified on the Y Chr. Two discrete amplified families of MuRVY retroviral genomes distinguishable by a polymorphic restriction site were detected, suggestive that amplification occurred in incremental stages. The presence of MuRVY-related DNA sequences, but absence of IAPE-Y-related DNA sequences in Mus spretus suggests that the IAPE-Y retro-transposition event occurred after the evolutionary divergence of the lineages leading to Mus musculus and Mus spretus, and that the amplification of MuRVY occurred independently in the two lineages.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Algate, P.A., McCubrey, JA. (1993). Automne transformation of hemopoietic cells resulting from cytokine message stabilization after in-tracisternal A particle transposition. Oncogene 8, 1221–1232.
Bishop, C.E., Hatat, D. (1987). Molecular cloning and sequence analysis of a mouse Y chromosome RNA transcript expressed in the testis. Nucleic Acids Res. 15, 2959–2969.
Blankenstein, T., Qin, Z., Li, W., Diamanstein, T. (1990). DNA rearrangement and constitutive expression of the interleukin 6 gene in a mouse plasmacytoma. J. Exp. Med. 171, 965–970.
Blatt, C., Aberdam, D., Schwartz, R., Sachs, L. (1988). DNA rearrangement of a homeobox gene in myeloid leukaemic cells. EMBO J. 7, 4283–4290.
Burt, D.W., Reith, A.D., Brammar, WJ. (1984). A retroviral provirus closely associated with the Ren-2 gene of DBA/2 mice. Nucleic Acids Res. 12, 8579–8593.
Canaani, E., Dreazen, O., Klar, A., Rechavi, G., Ram, D., Cohen, J.B., Givol, D. (1983). Activation of the c-mos oncogene in a mouse plasmacytoma by insertion of an endogenous intracisternal A-particle genome. Proc. Natl. Acad. Sci. USA 80, 7118–7122.
Delassus, S., Sonigo, P. Wain-Hobson, S. (1989). Genetic organization of Gibbon ape leukemia virus. Virology 173, 205–213.
Duhrsen, U., Stahl, J., Gough, N.M. (1990). In vivo transformation of factor-dependent hemopoietic cells: role of intracisternal A-particle transposition for growth factor gene activation. EMBO J. 9, 1087–1096.
Eicher, E.M., Hutchinson, K.W., Phillips, S.J., Tucker, P.K., Lee, B.K. (1989). A repeated segment on the mouse Y chromosome is composed of retroviral-related, Y-enriched and Y-specific sequences. Genetics 122, 181–192.
Eicher, E.M., Lee, B.K., Washburn, L.L., Hale, D.W., King, T.R. (1992). Telomere-related markers for the pseudoautosomal region of the mouse genome. Proc. Natl. Acad. Sci. USA 89, 2160–2164.
Fennelly, J., Crabtree, G., MacDonald, D., Lorimore, S., Laval, S., Proffitt, J., Boyd, Y., Wright, E., Plumb, M. (1995). Complex Y chromosome aberrations are a recurrent secondary event in radiation-induced murine acute myeloid leukaemia. Leukemia 9, 506–512.
Graves, J.A.M. (1995). The origin and function of the mammalian Y chromosome and Y-borne genes—an evolving understanding. BioEssays 17, 311–320.
Graves, J.A.M., Watson, J.M. (1991). Mammalian sex chromosomes: evolution of organization and function. Chromosoma 101, 63–68.
Gubbay, J., Lovell-Badge, R. (1994). The mouse Y chromosome. In: Molecular Genetics of Sex Determination. (San Diego: Academic Press, Inc.), pp. 43–67.
Hutchinson, K.W., Eicher, E.M. (1989). An amplified endogenous retro-viral sequence on the murine Y chromosome related to murine leukemia viruses and viruslike 30S sequences. J. Virol. 63, 4043–4046.
Kuff, E.L. (1990). Intracisternal A particles in mouse neoplasia. Cancer Cells 2, 398–400.
Kuff, E.L., Lueders, K.K. (1988). The intracisternal A-particle gene family: structure and functional aspects. Adv. Cancer Res. 51, 184–276.
Lamb, B.T., Satyamoorthy, K., Solter, D., Basu, A., Xu, M.Q., Weinmann, R., Howe, C.C. (1992). A DNA element that regulates expression of an endogenous retrovirus during F9 cell differentiation is ElA dependent. Mol. Cell. Biol. 12, 4824–4833.
Lueders, K.K., Kuff, E.L. (1977). Sequences associated with intracisternal A particles are reiterated in the mouse genome. Cell 12, 963–972.
Lueders, K.K., Fewell, J.W., Morozov, V.E., Kuff, E.L. (1993a). Selective expression of intracisternal A-particle genes in established mouse plas-macytomas. Mol. Cell. Biol. 13, 7439–7446.
Lueders, K.K., Frankel, W.N., Mietz, J.A., Kuff, E.L. (1993b). Genomic mapping of intracisternal A-particle proviral elements. Mamm. Genome 4, 69–77.
Mietz, J.A., Kuff, E.L. (1992). Intracisternal A-particle-specific oligonu-cleotides provide multilocus probes for genetic linkage studies in the mouse. Mamm. Genome 3, 447–451.
Mietiz, J.A., Grossman, Z., Lueders, K K., Kuff, E.L. (1987). Nucleotide sequence of a complete mouse intracisternal A-particle genome: relationship to known aspects of particle assembly and function. J. Virol. 61, 3020–3029.
Mietz, J.A., Fewell, J.W., Kuff, E.L. (1992). Selective activation of a discrete family of endogenous proviral elements in normal BALB/c lymphocytes. Mol. Cell. Biol. 12, 220–228.
Nishioka, Y., Dolan, B.M., Zahed, L., Prado, V., Tyson, H. (1994). Molecular evolution of a Y-chromosomal repetitive sequences family in the genus mus. Mol. Biol. Evol. 11, 146–153.
Pinkel, D., Straume, T., Gray, J.W. (1986). Cytogenetic analysis using quantitative, high-sensitivity fluorescence hybridization. Proc. Natl. Acad. Sci. USA 82, 2934–2938.
Reuss, F.U., Schaller, H.C. (1991). cDNA sequence and genomic organisation of intracisternal A-particle retroviral elements containing an envelope gene. J. Virol. 65, 5702–5709.
Roubertoux, P.L., Carlier, M., Degrelle, H., Haas-Dupertuis, M-C., Phillips, J., Moutier, R. (1994). Co-segregation of internal aggression with the pseudoautosomal region of the Y chromosome in mice. Genetics 135, 225–230.
Satyamoorthy, K., Park, K., Atchison, M.L., Howe, C.C. (1993). The intracisternal A-particle upstream element interacts with transcription factor YY1 to activate transcription: pleiotropic effects of YY1 on distinct DNA promoter elements. Mol. Cell. Biol. 13, 6621–6628.
Tucker, P.K., Lee, B.K., Eicher, E.M. (1989). Y chromosome evolution in the subgenus Mus (genus mus). Genetics 122, 169–170.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fennelly, J., Harper, K., Laval, S. et al. Co-amplification of tail-to-tail copies of MuRVY and IAPE retroviral genomes on the Mus musculus Y Chromosome. Mammalian Genome 7, 31–36 (1996). https://doi.org/10.1007/s003359900008
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s003359900008