Skip to main content
SpringerLink
Log in

Preliminary assessment of population structure in the mackerel icefish (Champsocephalus gunnari)

  • Original Paper
  • Published:
Polar Biology Aims and scope Submit manuscript

Abstract

The mackerel icefish (Champsocephalus gunnari Lönnberg E (1905) The Fishes of the Swedish South Polar Expedition. Wiss. Ergebnisse Schwedische Südpol- Exped. 1901–1903, vol 5, p 37 is widely distributed south of the Antarctic convergence and over shelf areas surrounding sub-Antarctic Islands. In order to evaluate global population structure in this species, we examined DNA sequence variation in four mitochondrial regions and four nuclear genes in icefish from four locations in the Atlantic Ocean sector and one location in the Indian Ocean. Despite small sample sizes, mitochondrial and nuclear gene data indicated the existence of at least three genetically distinct stocks: Heard Island, South Shetland Islands, and the remaining Atlantic populations (Shag Rocks, South Georgia, and Bouvet Island). The mitochondrial and nuclear SNP markers developed here will be useful for more extensive analyses of population structure in this species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Appleyard SA, Ward RD, Williams R (2002) Population structure of the Patagonian toothfish around Heard, McDonald and Macquarie Islands. Antarct Sci 14:364–373

    Article  Google Scholar 

  • Appleyard SA, Williams R, Ward RD (2004) Population genetic structure of Patagonian toothfish in the West Indian Ocean sector of the Southern Ocean. CCAMLR Sci 11:21–32

    Google Scholar 

  • Bandelt H-J, Forster P, Roehl A (1999) Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol 16:37–48

    PubMed  CAS  Google Scholar 

  • Bensch S, Åkesson S, Irwin D (2002) The use of AFLP to find an informative SNP: genetic differences across a migratory divide in willow warblers. Mol Ecol 11:2359–2366

    Article  PubMed  CAS  Google Scholar 

  • Brookes AJ (1999) Review: the essence of SNPs. Gene 234:177–186

    Article  PubMed  CAS  Google Scholar 

  • Brumfield RT, Beerli P, Nickerson DA, Edwards SV (2003) The utility of single nucleotide polymorphisms in inferences of population history. Trends Ecol Evol 18:249–256

    Article  Google Scholar 

  • Chow S (1998) Universal PCR primer for calmodulin gene intron in fish. Fish Sci 64:999–1000

    CAS  Google Scholar 

  • Chow S, Hazama K (1998) Universal PCR primers for S7 ribosomal protein gene introns in fish. Mol Ecol 7:1255–1256

    PubMed  CAS  Google Scholar 

  • Díez B, Massana R, Estrada M, Pedrós-Alió C (2004) Distribution of eukaryotic picoplankton assemblages across hydrographic fronts in the Southern Ocean, studied by denaturing gradient gel electrophoresis. Limnol Oceanogr 49:1022–1034

    Article  Google Scholar 

  • Duhamel G, Ozouf-Costaz C, Cattaneo-Berrebi G, Berrebi P (1995) Interpopulation relationships in two species of Antarctic fish Notothenia rossii and Champsocephalus gunnari from the Kerguelen Islands: an allozyme study. Antarct Sci 7:351–356

    Google Scholar 

  • Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinfo Online 1:47–50

    CAS  Google Scholar 

  • Fu YX (1997) Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics 147:915–925

    PubMed  CAS  Google Scholar 

  • Fu Y-X, Li W-H (1993) Statistical tests of neutrality of mutations. Genetics 133:693–709

    PubMed  CAS  Google Scholar 

  • Gubsch G (1982) Zur Verbreitung und zur Biologie der Eisfische (Chaenichthyidae) in atlantischen Sektor der Antarktis. Fischerei Forsch 20:39–47

    Google Scholar 

  • Guo S, Thompson E (1992) Performing the exact test of Hardy–Weinberg proportions for multiple alleles. Biometrics 48:361–372

    Article  PubMed  CAS  Google Scholar 

  • Hare MP, Palumbi SR (1999) The accuracy of heterozygous base calling from diploid sequence and resolution of haplotypes using allele-specific sequencing. Mol Ecol 8:1750–1752

    Article  PubMed  CAS  Google Scholar 

  • Iwami T, Kock K-H (1990) Channichthyidae. In: Gon O, Heemstra PC (eds) Fishes of the Southern Ocean. J.L.B. Smith Institute of Ichthyology, Grahamstown, South Africa, pp 381–399

    Google Scholar 

  • Kock K-H (1981) Fischereibiologische Untersuchungen an drei Antarktischen Fischarten: Champsocephalus gunnari Lönnberg, 1905, Chaenocephalus aceratus (Lönnberg, 1906), and Pseudochaenichthys georgianus Norman, 1937 (Notothenioidei, Channichthyidae). Mitteilungen aus dem Institut für Seefischerei (Hamburg) 32, 226 pp

  • Kock K-H (1992) Antarctic fish and fisheries. Cambridge University Press, Cambridge, 359 pp

  • Kock K-H (2005a) Antarctic icefishes (Channichthyidae: a unique family of fishes). A review, Part I. Polar Biol 28:862–895

    Article  Google Scholar 

  • Kock K-H (2005b) Antarctic icefishes (Channichthyidae: a unique family of fishes). A review, Part II. Polar Biol 28:897–909

    Article  Google Scholar 

  • Kock K-H, Everson I (1997) Biology and ecology of the mackerel icefish, Champsocephalus gunnari: an Antarctic fish lacking hemoglobin. Comp Biochem Phys A 118:1067–1077

    Article  Google Scholar 

  • Landegren U, Nilsson M, Kwok P-Y (1998) Reading bits of genetic information: methods for single nucleotide polymorphism analysis. Genome Res 8:769–776

    PubMed  CAS  Google Scholar 

  • Lönnberg E (1905) The Fishes of the Swedish South Polar Expedition. Wiss. Ergebnisse Schwedische Südpol- Exped. 1901-1903, vol 5, p 37

  • Martin AP, Palumbi SR (1993) Body size, metabolic rate, generation time, and the molecular clock. Proc Natl Acad Sci USA 90:4087–4091

    Article  PubMed  CAS  Google Scholar 

  • Meyer A, Kocher TD, Basasibwaki P, Wilson AC (1990) Monophyletic origin of Lake Victoria cichlid fishes suggested by mitochondrial DNA sequences. Nature 347:550–553

    Article  PubMed  CAS  Google Scholar 

  • Meyer A, Morrissey JM, Schartl M (1994) Recurrent origin of a sexually selected trait in Xiphophorus fishes inferred from a molecular phylogeny. Nature 368:539–542

    Article  PubMed  CAS  Google Scholar 

  • Morin P, Luikart G, Wayne R, the SNP Workshop Group (2004) SNPs in ecology, evolution and conservation. Trends Ecol Evol 19:208–216

    Article  Google Scholar 

  • North AW (2005) Mackerel icefish size and age differences and long-term change at South Georgia and Shag Rocks. J Fish Biol 67:1666–1685

    Article  Google Scholar 

  • Novelli VM, Takita MA, Machado MA (2004) Identification and analysis of single nucleotide polymorphisms (SNPs) in citrus. Euphytica 138:227–237

    Google Scholar 

  • Palumbi SR, Baker CS (1994) Contrasting population structure from nuclear intron sequences and mtDNA of humpback whales. Mol Biol Evol 11:426–435

    PubMed  CAS  Google Scholar 

  • Palumbi S, Martin S, Romano S, McMillan WO, Stice L, Grabowski G (1991) The simple fool’s guide to PCR, 2nd edn. Department of Zoology, University of Hawaii

  • Park LK, Brainard MA, Dightman DA, Winans GA (1993) Low levels of intraspecific variation in the mitochondrial DNA of chum salmon (Oncorhynchus keta). Mol Mar Biol Biotech 2:362–370

    CAS  Google Scholar 

  • Patarnello T, Marcato S, Zane L, Varotto V, Bargelloni L (2003) Phylogeography of the Chionodraco genus (Perciformes, Channichthyidae) in the Southern Ocean. Mol Phylogenet Evol 28:420–429

    Article  PubMed  CAS  Google Scholar 

  • Petit JR, Jouzel J, Raynaud D, Barkov NI, Barnola J-M, Basile I, Bender M, Chappellaz J, Davis M, Delaygue G, Delmotte M, Kotlyakov VM, Legrand M, Lipenkov VY, Lorius C, Pépin L, Ritz C, Saltzman E, Stievenard M (1999) Climate and atmospheric history of the past 420,000 years from the Vostok ice core, Antarctica. Nature 399:429–436

    Article  CAS  Google Scholar 

  • Rogers AR, Harpending H (1992) Population growth makes waves in the distribution of pairwise genetic differences. Mol Biol Evol 9:552–569

    PubMed  CAS  Google Scholar 

  • Rozas J, Sánchez-DelBarrio JC, Messeguer X, Rozas R (2003) DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics 19:2496–2497

    Article  PubMed  CAS  Google Scholar 

  • Schneider S, Excoffier L (1999) Estimation of past demographic parameters from the distribution of pairwise differences when the mutation rates vary among sites: application to human mitochondrial DNA. Genetics 152:1079–1089

    PubMed  CAS  Google Scholar 

  • Seddon JM, Parker HG, Ostrander EA, Ellegren H (2005) SNPs in ecological and conservation studies: a test in the Scandinavian wolf population. Mol Ecol 14:503–511

    Article  PubMed  CAS  Google Scholar 

  • Shaklee J, Bentzen P (1998) Genetic identification of stocks of marine fish and shellfish. Bull Mar Sci 62:589–621

    Google Scholar 

  • Shaw PW, Arkhipkin AI, Al-Khairulla H (2004) Genetic structuring of Patagonian toothfish populations in the Southwest Atlantic Ocean: the effect of the Antarctic Polar Front and deep-water troughs as barriers to genetic exchange. Mol Ecol 13:3293–3303

    Article  PubMed  CAS  Google Scholar 

  • Siegel V (1980) Parasite tags for some Antarctic channichthyid fish. Archiv für Fischereiwissenschaft 31:97–103

    Google Scholar 

  • Smith PJ, Gaffney PM, Purves M (2001) Genetic markers for identification of Patagonian toothfish and Antarctic toothfish. J Fish Biol 58:1190–1194

    Article  CAS  Google Scholar 

  • Smith CT, Baker J, Park L, Seeb W, Elfstrom C, Abe S, Seeb JE (2005) Characterization of 13 single nucleotide polymorphism markers for chum salmon. Mol Ecol Notes 5:259–262

    Article  CAS  Google Scholar 

  • Sosinski J (1985) Some data on taxonomy and biology of Antarctic fish, Champsocephalus gunnari Lönnberg, 1905. Acta Ichthyol Piscat 15:3–54

    Google Scholar 

  • Stephens M, Smith NJ, Donnelly P (2001) A new statistical method for haplotype reconstruction from population data. Am J Hum Genet 68:978–989

    Article  PubMed  CAS  Google Scholar 

  • Ward RD, Woodwark M, Skibinski D (1994) A comparison of genetic diversity levels in marine, freshwater and anadromous fish. J Fish Biol 44:213–232

    Article  Google Scholar 

  • Weir B, Cockerham C (1984) Estimating F-statistics for the analysis of population structure. Evolution 38:1358–1370

    Article  Google Scholar 

  • Williams R, Smolenski AJ, White RWG (1994) Mitochondrial DNA variation of Champsocephalus gunnari Lönnberg (Pisces: Channichthyidae) stocks on the Kerguelen Plateau, southern Indian Ocean. Antarct Sci 6:347–352

    Google Scholar 

  • Zane L, Ostellari L, Maccatrozzo L, Bargelloni L, Battaglia B, Patarnello T (1998) Molecular evidence for the genetic subdivision of Antarctic krill (Euphausia superba Dana) populations. Proc R Soc Lond B 265:2387–2391

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The ICEFISH cruise was supported by National Science Foundation grant OPP 01-32032 to H. William Detrich (Northeastern University). This is publication number 10 from the ICEFISH cruise of 2004 (H.W. Detrich, Chief Scientist). The authors would like to thank Timothy Targett, Guillaume Lecointre, Sharon Appleyard, and Robert Ward for tissue samples, and Sharon Appleyard, Karl-Hermann Kock, and one anonymous reviewer for their comments and suggestions.

Author information

Authors and Affiliations

  1. University of Delaware College of Marine Studies, 700 Pilottown Road, Lewes, DE, 19958, USA

    Kristen L. Kuhn & Patrick M. Gaffney

Authors
  1. Kristen L. Kuhn
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Patrick M. Gaffney
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kristen L. Kuhn.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kuhn, K.L., Gaffney, P.M. Preliminary assessment of population structure in the mackerel icefish (Champsocephalus gunnari). Polar Biol 29, 927–935 (2006). https://doi.org/10.1007/s00300-006-0134-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00300-006-0134-5

Keywords

Search

Navigation