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Differential accumulation of dehydrin-like proteins by abiotic stresses in Deschampsia antarctica Desv.

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Abstract

Dehydrins are proteins that accumulate during environmental stresses leading to cell dehydration. Deschampsia antarctica is one of the two vascular plants that have colonized the Maritime Antarctic. This plant is usually exposed to cold, salt and desiccating winds in the field. We proposed that among the factors that allow D. antarctica to survive the harsh environmental conditions is the presence of dehydrins. We studied the accumulation of dehydrins by abscisic acid (ABA), dehydration, NaCl and low osmotic potential. Western blots using an anti-dehydrin antibody revealed a complex pattern of dehydrin-like proteins (DLPs) accumulation in the different treatments. DLPs with apparent molecular weight of 58, 57, 55, 53, 48, 42, 32, 30, 28 and 25 kDa were detected in the different treatments. DLPs accumulation was associated with a decrease in the relative water content (RWC) of the plants. These results suggest that DLPs accumulation could contribute to explain how D. antarctica can survive under adverse Antarctic conditions.

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Acknowledgements

The financial support of FONDECYT 2000144 and fellowship from CONICYT are gratefully acknowledged. Instituto Antártico Chileno provided the logistics and the permit for collecting plants in an ASPA. We thank Dr. Timothy Close for providing the anti-dehydrin antibody.

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Authors and Affiliations

  1. Departamento de Botánica, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Casilla 160-C, Concepción, Chile

    Nélida Olave-Concha, León A. Bravo & Luis J. Corcuera

  2. Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Casilla 747, Talca, Chile

    Simón Ruiz-Lara

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  1. Nélida Olave-Concha
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  2. León A. Bravo
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  3. Simón Ruiz-Lara
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  4. Luis J. Corcuera
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Correspondence to Luis J. Corcuera.

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Olave-Concha, N., Bravo, L.A., Ruiz-Lara, S. et al. Differential accumulation of dehydrin-like proteins by abiotic stresses in Deschampsia antarctica Desv.. Polar Biol 28, 506–513 (2005). https://doi.org/10.1007/s00300-005-0718-5

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