Abstract
Key message
Numbers of critical genes and pathways were found from the levels of transcriptome and metabolome, which were useful information for understanding of kenaf CMS mechanism.
Abstract
Cytoplasmic male sterility (CMS) is a maternally inherited trait in higher plants that leads to the inability to produce or release functional pollen. However, there is lack of comprehensive studies to reveal the molecular basis of CMS occurrence in kenaf. Herein, we performed transcriptome and UPLC-MS-based metabolome analyses in the anthers of a CMS (UG93A) and its maintainer (UG93B) to sort out essential genes and metabolites responding to CMS in kenaf. Transcriptome characterized 7769 differentially expressed genes (DEGs) between these two materials, and pathway enrichment analysis indicated that these DEGs were involved mainly in pentose and glucuronate interconversions, starch and sucrose metabolism, taurine and hypotaurine metabolism. In the metabolome assay, a total of 116 significantly different metabolites (SDMs) were identified between the CMS and its maintainer line, and these SDMs were involved in eight KEGG pathways, including flavone and flavonol biosynthesis, glycerophospholipid metabolism, flavonoid biosynthesis, glycosylphosphatidylinositol-anchor biosynthesi. Integrated analyses of transcriptome and metabolome showed that 50 genes had strong correlation coefficient values (R2 > 0.9) with ten metabolites enriched in six pathways; notably, most genes and metabolites of flavonoid biosynthesis pathways and flavone and flavonol biosynthesis pathways involved in flavonoids biosynthetic pathways were downregulated in CMS compared to those in maintainer. Taken together, the decreased accumulation of flavonoids resulted from the compromised biosynthesis pathways coupled with energy deficiency in the anthers may contribute largely to CMS in UG93A of kenaf.
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Acknowledgements
We are thankful to Professor Ruiyang Zhou for providing seeds of kenaf cultivar UG93A and UG93B. This research work was supported by the National Natural Science Foundation of China (Grant nos. 31560421 and 31960368) and the earmarked fund for Modern Agro-industry Technology Research System (CARS-16-E14).
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PC conceived and designed the research. MT, ZL, DL and FW analyzed data and wrote the manuscript. MHK and HL revised the manuscript. LH, JY, ZH, WT and RL assist during the experiments. All authors read and approved the manuscript.
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Tang, M., Li, Z., Luo, D. et al. A comprehensive integrated transcriptome and metabolome analyses to reveal key genes and essential metabolic pathways involved in CMS in kenaf. Plant Cell Rep 40, 223–236 (2021). https://doi.org/10.1007/s00299-020-02628-7
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DOI: https://doi.org/10.1007/s00299-020-02628-7