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Fertile introgression products generated via somatic hybridization between wheat and Thinopyrum intermedium

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Abstract

Key message

Fertile hybrids were produced with genetic material transferred from Th. intermedium into a wheat background and supply a source of genetic variation to wheat improvement.

Abstract

Both symmetric and asymmetric somatic hybrids have been obtained from the combination of wheatgrass (Thinopyrum intermedium) and bread wheat (Triticum aestivum). Two wheat protoplast populations, one derived from embryogenic calli and the other from a non-regenerable, rapidly dividing cell line, were fused with Th. intermedium protoplasts which had been (or not been) pre-irradiated with UV. Among the 124 regenerated calli, 64 could be categorized as being of hybrid origin on the basis of plant morphology, peroxidase isozyme, RAPD DNA profiling and karyological analysis. Numerous green plantlets were regenerated from 13 calli recovered from either the symmetric hybrid (no UV pre-treatment) or the asymmetric one (30 s UV irradiation). One of these hybrid plants proved to be vigorous and self-fertile. The regenerants were all closer in phenotype to wheat than to Th. intermedium. Genomic in situ hybridization analysis showed that the chromosomes in the hybrids were largely intact wheat ones, although a few Th. intermedium chromosome fragments had been incorporated within them.

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Acknowledgments

This work was supported by the Natural Science Foundation of China (No. 31270385).

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Authors and Affiliations

  1. The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Jinan, 250100, People’s Republic of China

    Cuiling Li, Aixia Cheng, Minqin Wang & Guangmin Xia

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  1. Cuiling Li
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  2. Aixia Cheng
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  3. Minqin Wang
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  4. Guangmin Xia
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Corresponding author

Correspondence to Guangmin Xia.

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Communicated by K. Chong.

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Li, C., Cheng, A., Wang, M. et al. Fertile introgression products generated via somatic hybridization between wheat and Thinopyrum intermedium . Plant Cell Rep 33, 633–641 (2014). https://doi.org/10.1007/s00299-013-1553-8

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  • DOI: https://doi.org/10.1007/s00299-013-1553-8

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