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Genome-wide analysis of the CCCH zinc finger gene family in Medicago truncatula

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In this study, we identified 34 CCCH Znf genes in Medicago truncatula and the results of semi-quantitative RT-PCR revealed that the expression patterns of subfamily VI members were diverse.

Abstract

CCCH-type zinc finger (Znf) proteins are specific transcriptional factors with a typical motif consisting of three cysteine residues and one histidine residue. Increasing evidences have revealed that CCCH Znf proteins participated in the regulation of plant growth, developmental processes and environmental responses. Survey and characterization of CCCH Znf genes in leguminous species would facilitate a better understanding of the evolutionary processes and functions of this gene family. In this study, we performed a comprehensive analysis of CCCH Znf genes in M. truncatula by describing the phylogenetic relationships, chromosomal location and gene structure of each family member. A total of 34 CCCH Znf genes were identified in the latest M. truncatula genome sequence. The 34 predicted members were clustered into nine subfamilies based on their phylogenetic analysis and structure features. In addition, the 34 Medicago CCCH Znf genes were found to be unevenly distributed on eight chromosomes. Furthermore, the expression profiles of subfamily VI were investigated under different stress conditions (PEG-6000, NaCl and ABA) by using semi-quantitative RT-PCR. The data showed that these genes displayed different expression levels in response to various stress conditions. The results presented in this study provide basic information about Medicago CCCH Znf genes and form a fundamental clue for cloning genes with specific functions in further studies and applications.

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Acknowledgments

This work was supported by grants from the National Science Technology Support Program (No. 2009BADA6B06-1). We thank the members of the Laboratory of Modern Biotechnology for their assistance. We especially thank Guo Wei and Yuanhua Shao for their support and comments during the preparation of this manuscript.

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Authors and Affiliations

  1. Lab of Modern Biotechnology, School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei, 230036, China

    Cuiqin Zhang, Hongmei Zhang & Yan Xiang

  2. Key Lab of Biomass Improvement and Conversion, Anhui Agriculture University, Hefei, 230036, China

    Cuiqin Zhang, Yang Zhao, Haiyang Jiang, Suwen Zhu, Beijiu Cheng & Yan Xiang

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  1. Cuiqin Zhang
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  2. Hongmei Zhang
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  3. Yang Zhao
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  4. Haiyang Jiang
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  5. Suwen Zhu
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  6. Beijiu Cheng
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  7. Yan Xiang
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Correspondence to Yan Xiang.

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Communicated by Q. Zhao.

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Zhang, C., Zhang, H., Zhao, Y. et al. Genome-wide analysis of the CCCH zinc finger gene family in Medicago truncatula . Plant Cell Rep 32, 1543–1555 (2013). https://doi.org/10.1007/s00299-013-1466-6

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  • DOI: https://doi.org/10.1007/s00299-013-1466-6

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