Abstract
Key message
Highly variable regions of chloroplast genome were found to be useful in the detection of plant genetic diversity at micro-evolution level. Our methodology will improve understanding and conservation of plant diversity.
Abstract
Tree peonies are famous flowers with about 2,000 cultivars in the world, belonging to Paeonia sect. Moutan of the Paeoniaceae. They are traditionally classified based on flower forms and colors. Due to the limited number of DNA and morphological markers, and the existence of synonyms and homonyms, evaluation on genetic diversity of so many cultivars remains a challenge. In most cases, it is difficult and even impossible to discriminate tree peony cultivars when they are not in flower. In this study, single nucleotide polymorphism detected from the hyper-variable regions of chloroplast genome was employed to separate tree peony cultivars into different maternal lineages which can be expressed briefly by a nucleotide molecular formula. Our approach enabled a much higher resolution of cultivar identification and classification that has not been obtained before. The newly developed hyper-variable chloroplast markers, as an independent source of taxonomic characteristics, provided novel evidences and higher resolution ability that are helpful in building an effective classification system for evaluation, conservation, and utilization of the tree peony germplasm resources at cultivar level.
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Acknowledgments
This study was financially supported by the National Natural Science Foundation of China (Grant 30972412), by the Program of “Guidelines for the Techniques of Molecular Tests of New Plant Varieties” (No. 2007011) of the Science and Technology Development Centre of the State Forestry Administration of China and by the State Forestry Administration of China (Grant 2008-09). The authors thank the following experts for advice and help: Yuping Zou, Shiliang Zhou and Wenpan Dong in molecular experiments, Zhiming Zhang, Xiaoqing Zhao, Wenhai Sun, Jingyu Sun, Jinglian Sun, Baoli Sun, Huijin Zhang, Dezhong Chen, Fuhui Chen, Fufei Chen, Zhipeng Huo and Shulin Zhang in material collection.
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The authors declare that they have no conflict of interest.
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Communicated by J. R. Liu.
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Suo, Z., Zhang, C., Zheng, Y. et al. Revealing genetic diversity of tree peonies at micro-evolution level with hyper-variable chloroplast markers and floral traits. Plant Cell Rep 31, 2199–2213 (2012). https://doi.org/10.1007/s00299-012-1330-0
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DOI: https://doi.org/10.1007/s00299-012-1330-0