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Microarray analysis of differentially expressed genes between Brassica napus strains with high- and low-oleic acid contents

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Abstract

An increase in oleic acid (C18:1) content is a desirable trait. Despite the critical roles of the two desaturases, FAD2 and FAD3, in the control of fatty acid desaturation, a dispute remains over whether inactivation of their genes alone is sufficient enough to generate the high-oleic trait. To address this question, we employed microarray technology to investigate the difference in gene expression profile between two different Brassica napus strains with high-C18:1 (71.71%) and low-C18:1 (55.6%) contents, respectively. Our study revealed 562 differentially expressed genes, of which 194 genes were up-regulated and 368 down-regulated. Based on the Gene Ontology classification, these genes were classified into 23 functional categories. Three of the up-regulated genes represent B. napus homologs of Arabidopsis genes encoding a cytosolic isoform of pyruvate kinase (AT3G55810), Δ9 acyl-lipid desaturase (AT1G06080, ADS1) and fatty acyl-ACP thioesterase B (AT1G08510), respectively. Conversely, the homologs of two Arabidopsis sequences encoding Δ9 acyl-lipid desaturase (AT2G31360, ADS2) and FAD3 desaturase (AT2G29980) were down-regulated in the high-oleic acid strain. Furthermore, 60 differentially expressed genes were classified as associated with relevant Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Collectively, our results suggest that expressing the high-oleic acid trait may require a coordinated regulation of diverse regulatory and metabolic gene networks in addition to inactivation of the FAD2 and FAD3 genes in the oilseed. A set of the differentially expressed genes identified in this study will facilitate our efforts to tap the germplasms with the potential to express the high-oleic acid trait.

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References

  • Andre C, Froehlich JE, Moll MR, Benning C (2007) A heteromeric plastidic pyruvate kinase complex involved in seed oil biosynthesis in Arabidopsis. Plant Cell 19:2006–2022

    Article  PubMed  CAS  Google Scholar 

  • Bonaventure G, Salas JJ, Pollard MR, Ohlrogge JB (2003) Disruption of the FATB gene in Arabidopsis demonstrates an essential role of saturated fatty acids in plant growth. Plant Cell 15:1020–1033

    Article  PubMed  CAS  Google Scholar 

  • Del Río-Celestino M, Font R, Haro-Bailóna De (2007) Inheritance of high oleic acid content in the seed oil of mutant Ethiopian mustard lines and its relationship with erucic acid content. J Agric Sci 145:353–365

    Article  Google Scholar 

  • DeRisi JL, Lyer VR, Brown PO (1997) Exploring the metabolic and genetic control of gene expression on a genomic scale. Science 278:680–686

    Article  PubMed  CAS  Google Scholar 

  • Draghici S (2002) Statistical intelligence: effective analysis of high density microarray data. Drug Discov Today 7:S55–S66

    Article  PubMed  CAS  Google Scholar 

  • Fu S, Cheng H, Qi C (2009) Microarray analysis of gene expression in seeds of Brassica napus planted in Nanjing (altitude: 8.9 m), Xining (altitude: 2261.2 m) and Lhasa (altitude: 3658 m) with different oil content. Mol Biol Rep 36:2375–2386

    Article  PubMed  CAS  Google Scholar 

  • Fukuchi-Mizutani M, Tasaka Y, Tanaka Y, Ashikari T, Kusumi T, Murata N (1998) Characterization of delta 9 acyl-lipid desaturase homologues from Arabidopsis thaliana. Plant Cell Physiol 39:247

    PubMed  CAS  Google Scholar 

  • Giegé P, Heazlewood JL, Roessner-Tunali U, Millar AH, Fernie AR, Leaver CJ, Sweetlove LJ (2003) Enzymes of glycolysis are functionally associated with the mitochondrion in Arabidopsis cells. Plant Cell 15:2140–2151

    Article  PubMed  Google Scholar 

  • Girke T, Todd J, Ruuska S, White J, Benning C, Ohlrogge J (2000) Microarray analysis of developing Arabidopsis seeds. Plant Physiol 124:1570–1581

    Article  PubMed  CAS  Google Scholar 

  • Guan C (1985) Quality improvement and analysis method in rapeseed. Hunan Science and Technology Press, Hunan, pp 213–216

    Google Scholar 

  • Guan M (2004) High oleic acid oilseed rape breeding in Germany. Life Sci Res 8:28–32

    Google Scholar 

  • Guan C (2006) The research progress of genetic breeding in high oleic acid rapeseed. Crop Res 1:1–8

    Google Scholar 

  • Guan M, Li X (2009) Study on inheritance law of oleic acid character on Brassica napus. Life Sci Res 13:152–157

    CAS  Google Scholar 

  • Guan C, Liu C, Chen S, Peng Q, Li X, Guan M (2006) High oleic acid content materials of rapeseed (Brassica napus) produced by radiation breeding. Acta Agron Sin 32:1625–1629

    CAS  Google Scholar 

  • Horvath DP, Schaffer R, West M, Wisman E (2003) Arabidopsis microarrays identify conserved and differentially expressed genes involved in shoot growth and development from distantly related plant species. Plant J 34:125–134

    Article  PubMed  CAS  Google Scholar 

  • Hu X, Sullivan-Gilbert M, Gupta M, Thompson SA (2006) Mapping of the loci controlling oleic and linolenic acid contents and development of fad2 and fad3 allele-specific markers in canola (Brassica napus L.). Theor Appl Genet 113:497–507

    Article  PubMed  CAS  Google Scholar 

  • James DW, Dooner HK (1991a) Novel seed lipid phenotypes in combinations of mutants altered in fatty acid biosynthesis in Arabidopsis. Theor Appl Genet 82:409–412

    Article  CAS  Google Scholar 

  • James DW, Dooner HK (1991b) Isolation of EMS-induced mutants in Arabidopsis altered in seed fatty acid composition. Theor Appl Genet 80:241–245

    Google Scholar 

  • Javidfar F, Ripley VL, Roslinsky V, Zeinali H, Abdmishani C (2006) Identification of molecular markers associated with oleic and linolenic acid in spring oilseed rape (Brassica napus). Plant Breed 125:65–71

    Article  CAS  Google Scholar 

  • Jiang Y, Deyholos MK (2010) Transcriptome analysis of secondary-wall-enriched seed coat tissues of canola (Brassica napus L.). Plant Cell Rep 29:327–342

    Article  PubMed  CAS  Google Scholar 

  • Jones A, Davies HM, Voelker TA (1995) Palmitoyl-acyl carrier protein (ACP) thioesterase and the evolutionary origin of plant acyl-ACP thioesterases. Plant Cell 7:359–371

    Article  PubMed  CAS  Google Scholar 

  • Kachroo A, Shanklin J, Whittle E, Lapchyk L, Hildebrand D, Kachroo P (2007) The Arabidopsis stearoyl-acyl carrier protein-desaturase family and the contribution of leaf isoforms to oleic acid synthesis. Plant Mol Biol 63:257–271

    Article  PubMed  CAS  Google Scholar 

  • Kang J, Zhang G, Bonnema G, Fang Z, Wang X (2008) Global analysis of gene expression in flower buds of Ms-cd1 Brassica oleracea conferring male sterility by using an Arabidopsis microarray. Plant Mol Biol 66:177–192

    Article  PubMed  CAS  Google Scholar 

  • Li Y (2006) Gene chip data analysis and processing. Chemical Industry Press, Beijing, pp 120–121

    Google Scholar 

  • Liu R, Zhao J, Xiao Y, Meng J (2005) Identification of prior candidate genes for sclerotinia local resistance in Brassica napus using Arabidopsis cDNA microarray and Brassica–Arabidopsis comparative mapping. Sci China Ser C Life Sci 48:460–470

    Article  CAS  Google Scholar 

  • Peng Q, Hu Y, Wei R, Zhang Y, Guan C, Ruan Y, Liu C (2010) Simultaneous silencing of FAD2 and FAE1 genes affects both oleic acid and erucic acid contents in Brassica napus seeds. Plant Cell Rep 29:317–325

    Article  PubMed  CAS  Google Scholar 

  • Schena M, Shalon D, Heller R, Chai A, Brown PO, Davis RW (1996) Parallel human genome analysis: microarray-based expression monitoring of 1000 genes. Proc Natl Acad Sci 93:10614–10619

    Article  PubMed  CAS  Google Scholar 

  • Schierholt A, Becker HC, Ecke W (2000) Mapping a high oleic acid mutation in winter oilseed rape (Brassica napus L.). Theor Appl Genet 101:897–901

    Article  CAS  Google Scholar 

  • Schierholt A, Rücker B, Becker HC (2001) Inheritance of high oleic acid mutations in winter oilseed rape (Brassica napus L.). Crop Sci 41:1444–1449

    Article  CAS  Google Scholar 

  • Sivaraman I, Arumugam N, Sodhi YS, Gupta V, Mukhopadhyay A, Pradhan AK, Burma PK, Pental D (2004) Development of high oleic and low linoleic acid transgenics in a zero erucic acid Brassica juncea L. (Indian mustard) line by antisense suppression of the fad2 gene. Mol Breed 13:365–375

    Article  CAS  Google Scholar 

  • Stefan L, Paul C (2003) Analysing gene expression: a handbook of methods: possibilities and pitfalls. Wiley-VCH, Berlin, pp 287–370

    Google Scholar 

  • Stoutjesdijk PA, Hurlestone C, Singh SP, Green AG (2000) High-oleic acid Australian Brassica napus and B. juncea varieties produced by co-suppression of endogenous Δ12-desaturases. Biochem Soc Trans 28:938–940

    Article  PubMed  CAS  Google Scholar 

  • Stoutjesdijk PA, Singh SP, Liu Q, Hurlstone CJ, Waterhouse PA, Green AG (2002) hpRNA-mediated targeting of the Arabidopsis FAD2 gene gives highly efficient and stable silencing. Plant Physiol 129:1723–1731

    Article  PubMed  CAS  Google Scholar 

  • Tanhuanpää P, Vilkki J, Vihinen M (1998) Mapping and cloning of FAD2 gene to develop allele-specific PCR for oleic acid in spring turnip rape (Brassica rapa ssp. Oleifera). Mol Breed 4:543–550

    Article  Google Scholar 

  • Velasco L, Fernández-Martínez JM, de Haro A (2003) Inheritance of increased oleic acid concentration in high erucic acid Ethiopian mustard. Crop Sci 43:106–109

    Article  CAS  Google Scholar 

  • Xiao G, Zhang H, Peng Q, Guan C (2008) Screening and analysis of multiple copy of oleate desaturase gene (fad2) in Brassica napus. ACTA Agronomica Sinica 34:1563–1568

    Article  CAS  Google Scholar 

  • Buchanan BB, Gruissem W, Jones RL (2000) Biochemistry and molecular biology of plants. American Society of Plant Physiology, Rockville, pp 369–588

    Google Scholar 

  • Zhang H, Xiao G, Tan T, Li X, Guan C (2008) High oleate material of rapeseed (Brassica napus) produced by EMS treatment. Scientia Agricultura Sinica 41:4016–4022

    CAS  Google Scholar 

Download references

Acknowledgments

This research was financially supported by National Basic Research Program (2006CB101604), National High-tech R&D Program (2006AA101A113) from Ministry of Science and Technology of China.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. The Oilseed Crop Research Institute, National Oilseed Crop Improvement Center, Hunan Agricultural University, Changsha, 410128, China

    Mei Guan, Xun Li & Chunyun Guan

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  1. Mei Guan
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  2. Xun Li
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  3. Chunyun Guan
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Correspondence to Chunyun Guan.

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Communicated by J. Zou.

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Guan, M., Li, X. & Guan, C. Microarray analysis of differentially expressed genes between Brassica napus strains with high- and low-oleic acid contents. Plant Cell Rep 31, 929–943 (2012). https://doi.org/10.1007/s00299-011-1213-9

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  • DOI: https://doi.org/10.1007/s00299-011-1213-9

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