Abstract
Five intergeneric hybrids between the chrysanthemum cultivar ‘Zhongshanjingui’ (as female) and Ajania przewalskii (as male) were obtained with the help of embryo culture. While ‘Zhongshanjingui’ bears a standard anemone type flower and A. przewalskii a non-anemone type one, the inflorescence type of the hybrids varied. The diameter of the hybrids’ flowers was intermediate between those of the parents. The chromosome number of the hybrids was 2n = 45, of which GISH analysis was able to establish that 27 were inherited from ‘Zhongshanjingui’ and the other 18 from A. przewalskii. A combination of various assays was used to show that the cold tolerance of the hybrids was equivalent to that of the highly tolerant A. przewalskii parent. Enhanced cold tolerance was correlated with an increase in free proline and a decrease in malondialdehyde content.
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Abbreviations
- MA:
-
Mid-anemone type flower
- SA:
-
Standard anemone type flower
- NA:
-
Non-anemone type flower
- NER:
-
The number of emerged rhizomes
- GISH:
-
Genomic in situ hybridization
- EC:
-
Electrical conductivity
- REC:
-
The relative EC
- LT50 :
-
Semi-lethal low temperature
- Pro:
-
Proline
- MDA:
-
Malondialdehyde
- SR:
-
Survival rate
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Acknowledgments
This work was supported by the Basic Infrastructure Construction Plan of Science and Technology of Jiangsu Province (Grant No. BM2008008), the Shanghai Key Science and Technology for Agriculture Promotion Program (2009 No.3-3), the Fundamental Research Funds for the Central Universities (KYJ200907), Fund for Independent Innovation of Agricultural Sciences in Jiangsu Province (CX(10)114).
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Deng, Y., Chen, S., Chen, F. et al. The embryo rescue derived intergeneric hybrid between chrysanthemum and Ajania przewalskii shows enhanced cold tolerance. Plant Cell Rep 30, 2177–2186 (2011). https://doi.org/10.1007/s00299-011-1123-x
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DOI: https://doi.org/10.1007/s00299-011-1123-x