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Transcriptome profiling and insilico analysis of Gynostemma pentaphyllum using a next generation sequencer

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Abstract

Gynosaponins (Gypenosides) are major phyto-chemicals in Gynostemma pentaphyllum (Thunb.), with similarities to the ginsenosides present in Panax ginseng. Gynosaponins are classified as terpenoid compounds. In G. pentaphyllum, 25% of the total gynosaponins are similar to ginsenosides. In this study, we analyzed the transcriptional levels of the G. pentaphyllum genome to identify secondary metabolite genes. The complete transcriptomes for the roots and leaves were obtained using a GS-FLX pyro-sequencer. In total, we obtained 265,340 and all reads were well annotated according to biological databases. Using insilico analysis, 84% of sequence were well annotated and we obtained most of the secondary metabolite genes that represent mono-, di-, tri- and sesquiterpenoids. From our EST, most of the terpenoid genes were noted, among those few similar genes were studied in P. ginseng and these transcripts will help to characterize more triterpenoid genes in G. pentaphyllum. Also help to compare P. ginseng and G. pentaphyllum at transcriptome level.

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Acknowledgements

This research was supported by the MKE (Ministry of Knowledge Economy), Korea, under the ITRC (Information Technology Research Center) support program supervised by the NIPA (National IT Industry Promotion Agency)” (NIPA-2011-(C1090-1121-0003)).

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Authors and Affiliations

  1. Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University, Yongin, 449-701, South Korea

    Sathiyamoorthy Subramaniyam, Ramya Mathiyalagan, In Jun Gyo, Lee Bum-Soo & Yang Deok Chun

  2. Department of Computer Engineering, Kyung Hee University, Yongin, 449-701, South Korea

    Lee Sungyoung

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  1. Sathiyamoorthy Subramaniyam
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  2. Ramya Mathiyalagan
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  3. In Jun Gyo
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  4. Lee Bum-Soo
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  5. Lee Sungyoung
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  6. Yang Deok Chun
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Correspondence to Yang Deok Chun.

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Communicated by J. R. Liu.

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Subramaniyam, S., Mathiyalagan, R., Jun Gyo, I. et al. Transcriptome profiling and insilico analysis of Gynostemma pentaphyllum using a next generation sequencer. Plant Cell Rep 30, 2075–2083 (2011). https://doi.org/10.1007/s00299-011-1114-y

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