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Comparative assessment of genetic and epigenetic variation among regenerants of potato (Solanum tuberosum) derived from long-term nodal tissue-culture and cell selection

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Abstract

Three long-term nodal tissued cultured Russet Burbank potato clones and nine thaxtomin A-treated regenerant lines, derived from the nodal lines, were assessed for genetic and epigenetic (in the form of DNA methylation) differences by AFLP and MSAP. The treated regenerant lines were originally selected for superior resistance to common scab disease and acceptable tuber yield in pot and field trials. The long-term, tissue culture clone lines exhibited genetic (8.75–15.63% polymorphisms) and epigenetic (12.56–26.13% polymorphisms) differences between them and may represent a stress response induced by normal plant growth disruption. The thaxtomin A-treated regenerant lines exhibited much higher significant (p < 0.05) genetic (2–29.38%) and epigenetic (45.22–51.76%) polymorphisms than the nodal cultured parent clones. Methylation-sensitive mutations accumulated within the regenerant lines are significantly correlated (p < 0.05) to disease resistance. However, linking phenotypic differences that could be of benefit to potato growers, to single gene sequence polymorphisms in a tetraploid plant such as the potato would be extremely difficult since it is assumed many desirable traits are under polygenic control.

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Acknowledgments

The authors would like to thank Annabel Wilson for tissue culture and maintenance of all the potato lines, Dr. Robert Tegg for data and experimental information on field and pot trials, Peter Molesworth for help with statistical analysis and Adam Smolenski of the University of Tasmania, Central Science Laboratory for organising samples to be processed on the Beckman Coulter CEQ8000. This project had partial funding from an internal University of Tasmania grant.

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  1. Tasmanian Institute of Agricultural Research (TIAR), University of Tasmania, New Town Research Laboratories, 13 St Johns Ave, New Town, TAS, 7008, Australia

    Alison L. Dann & Calum R. Wilson

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  1. Alison L. Dann
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  2. Calum R. Wilson
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Correspondence to Calum R. Wilson.

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Communicated by H. Judelson.

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Dann, A.L., Wilson, C.R. Comparative assessment of genetic and epigenetic variation among regenerants of potato (Solanum tuberosum) derived from long-term nodal tissue-culture and cell selection. Plant Cell Rep 30, 631–639 (2011). https://doi.org/10.1007/s00299-010-0983-9

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  • DOI: https://doi.org/10.1007/s00299-010-0983-9

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