Skip to main content
SpringerLink
Log in

CaCl2 improves post-drought recovery potential in Camellia sinensis (L) O. Kuntze

  • Original Paper
  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Drought stress affects the growth and productivity of the tea plant. However, the damage caused is not permanent. The present investigation studies the effect of CaCl2 on antioxidative responses of tea during post-drought recovery. Increase in dry mass, proline and phenolic content of leaf with decrease in H2O2 and lipid peroxidation and increased activities of enzymes such as SOD, CAT, POX and GR in response to increased foliar CaCl2 concentration are indications for the recovery of stress-induced oxidative damage and thus improving post-stress recovery potential of Camellia sinensis genotypes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Asada K, Takahashi M (1987) Production and Scavenging of active oxygen in photosynthesis. In: Kyle DJ, Osmond CB, Arntzen CJ (eds), Photoinhibition. pp 227–287. Elsevier, Amsterdam; New York, Oxford

  • Bars HD, Weatherly PE (1962) A re-examinations of the relative turgidity technique for estimating water deficit in leaves. Aus J Biol Sci 15:413–428

    Google Scholar 

  • Basu S, Roychoudhury A, Saha PP, Sengupta DN (2010) Differential antioxidative responses of indica rice cultivars to drought stress. Plant Growth Regul 60:51–59

    Article  CAS  Google Scholar 

  • Bates LS, Waldern RP, Teare I K (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–208

    Google Scholar 

  • Bian S, Jiang Y (2009) Reactive oxygen species, antioxidant enzyme activities and gene expression patterns in leaves and roots of Kentucky bluegrass in response to drought stress and recovery. Sci Hort 120(2):264–270

    Article  CAS  Google Scholar 

  • Bouché N, Yellin A, Snedden WA, Fromm H (2005) Plant specific calmodulin-binding proteins. Ann Rev Plant Biol 56:435–466

    Article  Google Scholar 

  • Cabrera C, Artacho R, Gimenez R (2006) J Am Coll Nitr. Beneficial effect of green tea–a review 25(2):79–99

    CAS  Google Scholar 

  • Cousson A (2009) Involvement of phospholipase C-independent calcium-mediated abscisic acid signaling during Arabidopsis response to drought. Biol Plant 53(1):53–62

    Article  CAS  Google Scholar 

  • Evans NH, McAinsh MR, Hetherington AM, Knight MR (2005) ROS perception in Arabidopsis thaliana: the ozone induced calcium response. Plant J 41:615–626

    Article  PubMed  CAS  Google Scholar 

  • Farooqui AHA, Kumar R, Fatima S, Sharma S (2000) Responses of different genotypes of lemon grass (Cymbopogon flexuousus and C. Pendulus) to water stress. J Plant Biol 27:277–282

    Google Scholar 

  • Foyer CH, Lopez-Delgado H, Dat JF, Scott IM (1997) Hydrogen peroxide and glutathione-associated mechanisms of acclimatory stress tolerance and signaling. Physiol Plant 100:241–254

    Article  CAS  Google Scholar 

  • Giannopolitis CN, Ries SK (1997) Superoxide dismutase. I. occurrence in higher plants. Plant Physiol 59:309–314

    Article  Google Scholar 

  • Grattan SR, Grieve CM (1999) Salinity-mineral nutrient relations in horticultural crops. Sci Hortic 78(1–4):127–157

    CAS  Google Scholar 

  • Griffith OW (1980) Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Ann Biochem 106:207–211

    Article  CAS  Google Scholar 

  • Handique AC, Manivel L (1990) Selection criteria for drought tolerance in tea. Assam Rev Tea News 79:18–21

    Google Scholar 

  • Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198

    Article  PubMed  CAS  Google Scholar 

  • Herrig V, Ferrarese MLL, Suzuki LS, Rodrigues JD, Ferrarese-Filho O (2002) Peroxidase and phenylalanine ammonia-lyase activities, phenolic acid contents, and allelochemicals-inhibited root growth of soybean. Biol Res 35:59–66

    Google Scholar 

  • Jaleel CA, Manivannan P, Sankar B, Kishorekumar A, Gopi R, Somasundaram R, Panneerselvam R (2007) Water deficit stress mitigation by calcium chloride in Catharanthus roseus: effects on oxidative stress, proline metabolism and indole alkaloid accumulation. Colloids Surf B Biointerfaces 60:110–116

    Article  PubMed  CAS  Google Scholar 

  • Jeyaramraya PR, Roy RK, Pius PK, Thomas J (2003) Photoassimilatory and photorespiratory behaviour of certain drought tolerant and susceptible tea genotypes. Photosynthetica 41(4):579–582

    Article  Google Scholar 

  • Kar M, Mishra D (1976) Catalase, peroxidase and polyphenol oxidase activities during rice leaf senescence. Plant Physiol 57:315–319

    Article  PubMed  CAS  Google Scholar 

  • Kathju S, Vyas SP, Garg BK, Lahiri AN (1988) Fertility induced improvements in performance and metabolism of wheat under different intensities of water stress. In: Proceedings of the International Congress of Plant Physiology, New Delhi, pp 854–858

  • Kaya C, Higgs D (2002) Calcium nitrate as a remedy for salt-stressed cucumber plants. J Plant Nutr 25(4):861–871

    Article  CAS  Google Scholar 

  • Kaya C, Kirnak H, Higgs D (2001) Enhancement of growth and normal growth parameters by foliar application of potassium and phosphorus in tomato cultivars grown at high (NaCl) salinity. J Plant Nutr 24(2):357–367

    Article  CAS  Google Scholar 

  • Khan MN, Siddiqui MH, Mohammad F, Naeem M, Khan MMA (2010) Calcium chloride and gibberellic acid protect linseed (Linum usitatissimum L.) from NaCl stress by inducing antioxidative defence system and osmoprotectant accumulation. Acta Physiol Plant 32:121–132

    Google Scholar 

  • Kolupaev YE, Akinina GE, Mokrousov AV (2005) Induction of heat tolerance in wheat coleoptiles by calcium ions and its relation to oxidative stress. Russ J Plant Physiol 52:199–204

    Article  CAS  Google Scholar 

  • Levitt J (1980) Responses of plants to environmental stress, vol I. Academic Press, London

  • Lu S, Su W, Li H, Guo Z (2009) Abscisic acid improves drought tolerance of triploid bermudagrass and involves H2O2- and NO-induced antioxidant enzyme activities. Plant Physiol Biochem 47(2):132–138

    Article  PubMed  CAS  Google Scholar 

  • Luo Y, Zhao X, Zhou R, Zuo X, Zhang J, Li Y (2010) Physiological acclimation of two psammophytes to repeated soil drought and rewatering. Acta Physiol Plant. doi:10.1007/s11738-010-0519-5

  • Matkowski A, Wolniak D (2005) Plant phenolic metabolites as the free radical scavenger and mutagenesis inhibitors. BMC Plant Biol 5:S23

    Article  Google Scholar 

  • Matysik J, Ali BB, Mohanty P (2002) Molecular mechanism of quenching of reactive oxygen species by proline under water stress in plants. Curr Sci 82:525–532

    CAS  Google Scholar 

  • Nayyar H, Kaushal SK (2002) Alleviation of negative effects of water stress in two contrasting wheat genotypes by calcium and abscisic acid. Biol Plant 45(1):65–70

    Article  CAS  Google Scholar 

  • Noctor G (2006) Metabolic signalling in defence and stress: the central roles of soluble redox couples. Plant Cell Envt 29:409–425

    Article  CAS  Google Scholar 

  • Oser BL (1979) Hawks physiological chemistry. Mc Graw Hill, NewYork, pp 702–705

  • Rentel MC, Knight MR (2004) Oxidative stress-induced calcium signaling in Arabidopsis. Plant Physiol 135:1471–1479

    Article  PubMed  CAS  Google Scholar 

  • Ruiz JM, Rivero RM, Lo′ pez-Cantarero I, Romero L (2003) Role of Ca2+ in the metabolism of phenolic compounds in tobacco leaves (Nicotiana tabacum L.). Plant Growth Reg 41:73–177

    Google Scholar 

  • Sagisaka S (1976) The occurrence of peroxide in a perennial plant Populas gelrica. Plant Physiol 57:308–309

    Article  PubMed  CAS  Google Scholar 

  • Sanders D, Pelloux J, Brownlee C, Harper JF (2002) Calcium at the crossroads of signaling. Plant Cell 14:S401–S417

    PubMed  CAS  Google Scholar 

  • Sang S, Yang SC, Chi-Tang Ho (2004) Peroxidase mediated oxidation of catechins. Phytochem Rev 3:229–241

    Article  CAS  Google Scholar 

  • Shao HB, Song WY, Chu LY (2008) Advances of calcium signals involved in plant anti-drought. C R Biologies 331:587–596

    Article  PubMed  CAS  Google Scholar 

  • Siddiqui MH, Al-Whaibi MB, Basalah MO (2010) Interactive effect of calcium and gibberellin on nickel tolerance in relation to antioxidant systems in Triticum aestivum L. Protoplasma. doi:10.1007/s00709-010-0197-6

  • Smith IK, Vierheller TL, Thorne CA (1988) Assay of glutathione reductase in crude tissue homogenates using 5, 5′-dithiobis(2-nitrobenzoic acid). Anal Biochem 175:408–413

    Article  PubMed  CAS  Google Scholar 

  • Srivastava RC, Husain MM, Hasan SK, Mohammad A (2000) Green tea polyphenols and tannic acid act as potent inhibitors of phorbol ester-induced nitric oxide generation in rat hepatocytes independent of their antioxidant properties. Cancer Lett 153:1–5

    Article  PubMed  CAS  Google Scholar 

  • Teixeira AF, de Bastos Andrade A, Ferrarese-Filho O, Lucio Ferrarese ML (2006) Role of calcium on phenolic compounds and enzymes related to lignification in soybean (Glycine max L.) root growth. Plant Growth Reg 49:69–76

    CAS  Google Scholar 

  • Upadhyaya H, Panda SK (2004) Responses of Camellia sinensis to drought and rehydration. Biol Plant 48(4):597–600

    Article  Google Scholar 

  • Upadhyaya H, Khan MH, Panda SK (2007) Hydrogen peroxide induces oxidative stress in detached leaves of Oryza sativa. Gen Apll Plant Physiol 33(1–2):83–95

    CAS  Google Scholar 

  • Upadhyaya H, Panda SK, Dutta BK (2008) Variation of physiological and antioxidative responses in tea cultivars subjected to elevated water stress followed by rehydration recovery. Acta Physiol Plant 30:457–468

    Article  CAS  Google Scholar 

  • Verbruggen N, Hermans C (2008) Proline accumulation in plants: a review. Amino Acids 35:753–759

    Article  PubMed  CAS  Google Scholar 

  • Vomáčka L, Pospíšilová J (2003) Rehydration of sugar beet plants after water stress: effect of cytokinins. Biol Plant 46(1):57–62

    Google Scholar 

  • Wang CQ, Song H (2009) Calcium protects Trifolium repens L. seedlings against cadmium stress. Plant Cell Rep. doi:10.1007/s00299-009-0734-y

  • Wang CQ, Wang BS (2007) Ca2+-Calmodulin is involved in betacyanin accumulation induced by darkness in C3 halophyte Suaeda salsa. J Integr Plant Biol 49:1378–1385

    Article  CAS  Google Scholar 

  • Wang ZY, Cheng SJ, Zhou ZC, Athar M, Khan WA, Bickers DR, Mukhtar H (1989) Antimutagenic activity of green tea polyphenols. Mutat Res 223:273–285

    Article  PubMed  CAS  Google Scholar 

  • Wang CQ, Zhang YF, Liu T (2005) Activity changes of calmodulin and Ca2+-ATPase during low temperature-induced anthocyanin accumulation in Alternanthera bettzickiana. Physiol Plant 124:260–266

    Article  CAS  Google Scholar 

  • Xu J, Yin HX, Li X (2009) Protective effects of proline against cadmium toxicity in micropropagated hyperaccumulator, Solanum nigrum L. Plant Cell Rep 28:325–333

    Article  PubMed  CAS  Google Scholar 

  • Yoshiba Y, Kiyosue T, Nakashima K, Yamaguchi SK, Shinozaki K (1997) Regulation of level of proline as an osmolyte in plant under water stress. Plant Cell Physiol 18:1095

    Google Scholar 

Download references

Acknowledgments

Financial support for the research from the National Tea Research Foundation (NTRF), Kolkatta, (NTRF: 107/07), is gratefully acknowledged. The authors also thank Mr. I. B Ubadhia, Manager, Rosekhandy Tea Estate for providing tea seedlings throughout the experimental work.

Author information

Author notes

  1. Hrishikesh Upadhyaya

    Present address: Department of Botany and Biotechnology, Karimganj College, Karimganj, Assam, India

Authors and Affiliations

  1. Plant Biochemistry and Molecular Biology Laboratory, School of Life Sciences, Assam (Central) University, Silchar, 788011, India

    Hrishikesh Upadhyaya & Sanjib Kumar Panda

  2. Microbial and Agricultural Ecology Laboratory, Department of Ecology and Environmental Sciences, Assam (Central) University, Silchar, 788011, India

    Biman Kumar Dutta

Authors
  1. Hrishikesh Upadhyaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sanjib Kumar Panda
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Biman Kumar Dutta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hrishikesh Upadhyaya.

Additional information

Communicated by P. Kumar.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Upadhyaya, H., Panda, S.K. & Dutta, B.K. CaCl2 improves post-drought recovery potential in Camellia sinensis (L) O. Kuntze. Plant Cell Rep 30, 495–503 (2011). https://doi.org/10.1007/s00299-010-0958-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00299-010-0958-x

Keywords

Search

Navigation