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S locus-linked F-box genes expressed in anthers of Hordeum bulbosum

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Abstract

Diploid Hordeum bulbosum (a wild relative of cultivated barley) exhibits a two-locus self-incompatibility (SI) system gametophytically controlled by the unlinked multiallelic loci S and Z. This unique SI system is observed in the grasses (Poaceae) including the tribe Triticeae. This paper describes the identification and characterization of two F-box genes cosegregating with the S locus in H. bulbosum, named Hordeum S locus-linked F-box 1 (HSLF1) and HSLF2, which were derived from an S 3 haplotype-specific clone (HAS175) obtained by previous AMF (AFLP-based mRNA fingerprinting) analysis. Sequence analysis showed that both genes encode similar F-box proteins with a C-terminal leucine-rich repeat (LRR) domain, which are distinct from S locus (or S haplotype-specific) F-box protein (SLF/SFB), a class of F-box proteins identified as the pollen S determinant in S-RNase-based gametophytic SI systems. A number of homologous F-box genes with an LRR domain were found in the rice genome, although the functions of the gene family are unknown. One allele of the HSLF1 gene (HSLF1-S 3) was expressed specifically in mature anthers, whereas no expression was detected from the other two alleles examined. Although the degree of sequence polymorphism among the three HSLF1 alleles was low, a frameshift mutation was found in one of the unexpressed alleles. The HSLF2 gene showed a low level of expression with no tissue specificity as well as little sequence polymorphism among the three alleles. The multiplicity of S locus-linked F-box genes is discussed in comparison with those found in the S-RNase-based SI system.

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Acknowledgments

I thank M. Konoura, M. Kawai, K. Ohgishi and Y. Ishihama for technical assistance. This work was supported by Grant-in-Aids for Scientific Research C (Nos. 15580006 and 21580006) from the Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT).

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Authors and Affiliations

  1. Graduate School of Bioresources, Mie University, Tsu, 514-8507, Japan

    Katsuyuki Kakeda

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  1. Katsuyuki Kakeda
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Correspondence to Katsuyuki Kakeda.

Additional information

Communicated by R. Schmidt.

Nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession numbers AB511822–AB511825 and AB511859–AB511862.

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Kakeda, K. S locus-linked F-box genes expressed in anthers of Hordeum bulbosum. Plant Cell Rep 28, 1453–1460 (2009). https://doi.org/10.1007/s00299-009-0745-8

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  • DOI: https://doi.org/10.1007/s00299-009-0745-8

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