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Overexpression of lipid transfer protein (LTP) genes enhances resistance to plant pathogens and LTP functions in long-distance systemic signaling in tobacco

  • Genetic Transformation and Hybridization
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Abstract

The lipid signal is essential for the activation of plant defense responses, but downstream components of the signaling pathway are still poorly defined. To investigate the biological functions of pepper lipid transfer protein (LTP), we carried out virus-induced gene silencing (VIGS) in pepper, constitutive expression of CALTPs and grafting experiments in the tobacco plant. Suppression of endogenous CALTPI and CALTPII by VIGS, respectively, resulted in enhanced susceptibility to Xanthomonas campestris pv. vescatoria and pepper mosaic mottle virus in pepper. On the other hand, the constitutive expression of CALTPI and CALTPII genes in tobacco plants showed enhanced resistance to oomycete pathogen, Phytophthora nicotianae and bacterial pathogen, Pseudomonas syringae pv. tabaci. Enhanced resistance is found to be associated with the enhanced CALTP transcript levels in the independent transgenic CALTPI or II tobacco lines. Induced resistance responses in grafted scion leaves revealed that LTP plays a role in long-distance systemic signaling in plants.

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Acknowledgments

We thank Dr. S.P. Dinesh-Kumar (Yale University) for the pTRV1 and pTRV2 vectors. This study was supported by the special grant from the Agricultural R&D Promotion Center funded by the Ministry of Agriculture and Forestry, by a grant (code CG3112) from the Crop Functional Genomics Center of the 21st Century Frontier Research Program and by a grant from the SIGNET (R11-2003-008-04006) and SIGNET (R11-2003-008-02006) funded by the Ministry of Science and Technology (MOST), Republic of Korea.

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  1. Sujon Sarowar

    Present address: Department of Crop Science, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA

Authors and Affiliations

  1. School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, South Korea

    Sujon Sarowar, Young Jin Kim, Ki Deok Kim, Byung Kook Hwang, Sung Han Ok & Jeong Sheop Shin

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  1. Sujon Sarowar
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  2. Young Jin Kim
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Corresponding author

Correspondence to Jeong Sheop Shin.

Additional information

Communicated by J. R. Liu.

Sujon Sarowar and Young Jin Kim contributed equally to this study.

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Sarowar, S., Kim, Y.J., Kim, K.D. et al. Overexpression of lipid transfer protein (LTP) genes enhances resistance to plant pathogens and LTP functions in long-distance systemic signaling in tobacco. Plant Cell Rep 28, 419–427 (2009). https://doi.org/10.1007/s00299-008-0653-3

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  • DOI: https://doi.org/10.1007/s00299-008-0653-3

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