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Characterization of cytoplasmic male sterility of rice with Lead Rice cytoplasm in comparison with that with Chinsurah Boro II cytoplasm

  • Genetics and Genomics
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Abstract

Rice with LD-type cytoplasmic male sterility (CMS) possesses the cytoplasm of ‘Lead Rice’ and its fertility is recovered by a nuclear fertility restorer gene Rf1. Rf1 promotes processing of a CMS-associated mitochondrial RNA of atp6–orf79, which consists of atp6 and orf79, in BT-CMS with the cytoplasm of ‘Chinsurah Boro II’. In this study, we found that LD-cytoplasm contained a sequence variant of orf79 downstream of atp6. Northern blot analysis showed that atp6–orf79 RNA of LD-cytoplasm was co-transcribed and was processed in the presence of Rf1 in the same manner as in BT-cytoplasm. Western blot analysis showed that the ORF79 peptide did not accumulate in an LD-CMS line, while ORF79 accumulated in a BT-CMS line and was diminished by Rf1. These results suggest that accumulation of ORF79 is not the cause of CMS in LD-cytoplasm and the mechanism of male-sterility induction/fertility restoration in LD-CMS is different from that in BT-CMS.

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Abbreviations

CMS:

Cytoplasmic male sterility

PPR:

Pentatricopeptide repeat

Rf :

Fertility restorer gene

T65:

Taichung 65

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Acknowledgments

This study was partially supported by a Grant-in-Aid for Special Research on Priority Areas (No. 18075002) from the Ministry of Education, Science, Sports and Culture, Japan, and by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Green Technology Project QT-2007). We are grateful to Dr. Kiyoyuki Miura at National Agricultural Research Center and Dr. Hirokazu Handa at National Institute of Agrobiological Sciences for providing seeds of LD-CMS lines and anti-COX2 antiserum, respectively. E. I. is the recipient of Research Fellowships of the Japan Society for the promotion of Science for Young Scientists.

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Authors and Affiliations

  1. Laboratory of Environmental Biotechnology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, 981-8555, Japan

    Etsuko Itabashi, Tomohiko Kazama & Kinya Toriyama

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  1. Etsuko Itabashi
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  2. Tomohiko Kazama
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  3. Kinya Toriyama
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Correspondence to Kinya Toriyama.

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Communicated by F. Sato.

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Itabashi, E., Kazama, T. & Toriyama, K. Characterization of cytoplasmic male sterility of rice with Lead Rice cytoplasm in comparison with that with Chinsurah Boro II cytoplasm. Plant Cell Rep 28, 233–239 (2009). https://doi.org/10.1007/s00299-008-0625-7

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  • DOI: https://doi.org/10.1007/s00299-008-0625-7

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