Abstract
Genetic transformation is often associated with different rearrangements of the plant genome at the site of insertion. Therefore the question remains weather these T-DNA insertion sites are more prone to genotoxic stresses. Here, we studied the impact of propagation through generations, the influence of gene stacking and of photo oxidative stress caused by high light intensity on the stability of the transgene flanking regions in the model plant Arabidopsis thaliana. Conformational Sensitive Capillary Electrophoresis (CSCE), RFLP and sequencing were deployed in this analysis in order to study the proximal 100 bp and the long-range T-DNA flanking sequences. By screening seven transgenic lines no evidence for occurrence of mutation events were found, implying that the nucleotide sequence of the T-DNA flanking regions of the studied events is unlikely to be unstable.
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Acknowledgments
This work is performed in the frame of project “Development of methods for the detection, identification and quantification of genetically modified organisms”, contact No. 18687-2001-11 F1ED ISP BE, funded by European Commission. We are grateful to Evelien Berghman, Kristien Janssens and Cindy Merckaert for their excellent contribution.
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Communicated by P. Puigdomenech.
N. Papazova and R. Ghedira have equally contributed to the paper.
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Papazova, N., Ghedira, R., Van Glabeke, S. et al. Stability of the T-DNA flanking regions in transgenic Arabidopsis thaliana plants under influence of abiotic stress and cultivation practices. Plant Cell Rep 27, 749–757 (2008). https://doi.org/10.1007/s00299-007-0495-4
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DOI: https://doi.org/10.1007/s00299-007-0495-4